FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7070260
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG78158311.054515675519712No Hit
CAACGGAATCCCAAAAGCAGCTG6658859.418111922333832No Hit
CCCCCACTGCTAAATTTGACTG2244233.174183127636042No Hit
AACATTCAACGCTGTCGGTGAGT2121323.0003422787846556No Hit
TTCAAGTAATCCAGGATAGGCT2067542.9242771835830648No Hit
TAGCAGCACGTAAATATTGGCG2006722.8382548873733073No Hit
AACATTCAACGCTGTCGGTGAG1544772.1848842899695344No Hit
AAGCTGCCAGTTGAAGAACTGT1473062.0834594484502693No Hit
TATTGCACTTGTCCCGGCCTGTAA1134731.604933906249558No Hit
TATTGCACTTGTCCCGGCCTGTAT1010881.429763544763559No Hit
TAGCTTATCAGACTGATGTTGAC925581.309117345048131No Hit
TAGCTTATCAGACTGATGTTGACT919351.300305787905961No Hit
TAGCTTATCAGACTGATGTTGA828201.1713854936027812No Hit
TGAGGTAGTAGGTTGTATAGTT759811.0746563775589584No Hit
GGAATACCGGGTGCTGTAGGCTTT645470.9129367236848432No Hit
TATTGCACTTGTCCCGGCCTGT617670.8736170947037307No Hit
GGAATACCGGGTGCTGTAGGCTT483130.6833270629368652No Hit
TGAGGTAGTAGATTGTATAGTT443430.6271763697516074No Hit
TATTGCACTTGTCCCGGCCTGTA430610.6090440804157131No Hit
TGTAAACATCCTTGACTGGAAGCT407130.5758345520532484No Hit
TTCAAGTAATTCAGGATAGGTT394700.5582538690232042No Hit
CAACGGAATCCCAAAAGCAGCTGT367190.5193444088336213No Hit
AGCAGCATTGTACAGGGCTATGA342080.4838294489877317No Hit
ACTGGACTTGGAGTCAGAAGGC340550.48166545501862734No Hit
CCCCCACTGCTAAATTTGACTGGCTT327850.46370289069991766No Hit
TGTAAACATCCCCGACTGGAAGCT322740.45647543371813765No Hit
CATAAAGTAGAAAGCACTACT320210.45289706460582785No Hit
TTCTCACTACTGCACTTGACTAG315200.44581104513836833No Hit
TGAATACCGGGTGCTGTAGGCTTT279170.39485110872867474No Hit
CTCCCACTGCTTCACTTGACTAG273630.38701547043531637No Hit
CTTCTCACTACTGCACTTGACTAG259730.36735565594476016No Hit
ACCCCCCACTGCTAAATTTGACTG231700.3277107206807105No Hit
CACCCGGCTGTGTGCACATGTGC226800.3207802824790036No Hit
TCGTACGACTCTTAGCGG222020.3140195692944814No Hit
TTAATGCTAATCGTGATAGGGGTT218800.3094652813333597No Hit
CAACGGAATCCCAAAAGCAGCT217750.307980187432994No Hit
TGAATACCGGGTGCTGTAGGCTT209100.29574584244426655No Hit
CATTGCACTTGTCTCGGTCTGA208940.29551954242135364No Hit
CCCCCCACTGCTAAATTTGACTGG203270.28750003535937857No Hit
CCCCCCACTGCTAAATTTGACTGGCT200100.2830164661554172No Hit
GCCTGTCTGAGCGTCGCT199440.2820829785609016No Hit
CCCATAAAGTAGAAAGCACTAC199080.28157380350934763No Hit
AACCGGGCGGAAACACCA189690.2682928209146481No Hit
TGAGGTAGTAGTTTGTACAGTT164540.23272128606303022No Hit
CAAAGAATTCTCCTTTTGGGCT160820.2274598105303058No Hit
CTCCCACTGCTTCACTTGACTAGCCT160760.22737494802171349No Hit
CCCATAAAGTAGAAAGCACT150040.21221284648655073No Hit
TAGCAGCACGTAAATATTGGC147570.20871933988283317No Hit
TAGCTTATCAGACTGATGTTGACA139100.1967395824198827No Hit
TATTGCACTTGTCCCGGCCTGTAG133440.18873421910933968No Hit
CAACACCAGTCGATGGGCTGT129270.18283627476217282No Hit
AACGGAATCCCAAAAGCAGCTG128240.18137946836467117No Hit
AACATTCAACGCTGTCGGTGAGTT127920.1809268683188454No Hit
TGATGTAGTAGGTTGTATAGTT127830.18079957455595694No Hit
GACCCGCGGGCGCTCTCCAGTCCT123340.17444903016296429No Hit
CACTAGATTGTGAGCTCCTGGA119040.16836721704718072No Hit
TGAGGTAGTAGTTTGTGCTGTT118200.16717914192688813No Hit
AGCTCGGTCTGAGGCCCCTCAGT117980.1668679793953829No Hit
TATTGCACTTGTCCCGGCCTGTT117330.16594863555229936No Hit
CAAAGAATTCTCCTTTTGGGCTT116340.1645484041605259No Hit
CACTAGATTGTGAGCTCCTGGAA107090.1514654340858752No Hit
TGTAAACATCCCCGACTGGAAGC103910.14696772113048176No Hit
GGAATACCGGGTGCTGTAGGCTTA102650.14518560845004286No Hit
CGCCCCACTGCTAAATTTGACTG101820.1440116770811823No Hit
TTCAAGTAATCCAGGATAGGCTT101400.143417639521036No Hit
TGAGAACTGAATTCCATGGGTT100650.1423568581636319No Hit
TGTAAACATCCTACACTCTCAGCT99720.1410414892804508No Hit
CTACGCCTGTCTGAGCGTCGCT96220.1360911762792316No Hit
ACTGGACTTGGAGTCAGAAGGCT95420.13495967616466722No Hit
TATTGCACTTGTCCCGGCCTGTAAT95230.13469094488745817No Hit
ACTGGACTTGGAGTCAGAAGGCA92700.1311125757751483No Hit
TTCAAGTAATCCAGGATAGGC90180.12754835041427048No Hit
CCCCCCACTGCTAAATTTGACTGT89440.12650171280829842No Hit
CCCCCCACTGCTAAATTTGACTGTA89070.12597839400531238No Hit
CGCCTGTCTGAGCGTCGCT88220.12477617513358774No Hit
TCGTTTCCCGGCCAATGCACCA87420.12364467501902335No Hit
CCCGCCACTGCTAAATTTGACTG85890.12148068104991896No Hit
ACTTGACTTGGAGTCAGAAGGC85720.12124023727557404No Hit
TATTGCACTTGTCCCGGCCTGTTT85060.12030674968105841No Hit
CGCGACCTCAGATCAGACGTGGCG84100.11894894954358115No Hit
CAACGGAATCCCAAAAGCAGCTGA82980.117364849383191No Hit
TGAGGTAGTAGGTTGTGTGGTT82910.11726584312316661No Hit
TCCTGTCTGAGCGTCGCT82300.11640307428581126No Hit
TTCACAGTGGCTAAGTTCTGC81900.11583732422852909No Hit
GGAATACCGGGTGCTGTAGGCT79450.11237210512767565No Hit
TATTGCACTTGTCCCGGCCTGTAAA75520.10681361081487808No Hit
TGATGTAGTAGATTGTATAGTT74090.10479105436009424No Hit
CCCCCACTGCTAAATTTGACTGGCT73900.10452232308288523No Hit
TGTCAGTTTGTCAAATACCCCAA73300.10367369799696192No Hit
ATGCACCTGGGCAAGGATTCTGA70740.1000528976303559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)