FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10997137
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8302327.54952857275489No Hit
CAACGGAATCCCAAAAGCAGCTG7013676.377723583874603No Hit
AACATTCAACGCTGTCGGTGAGT4335963.9428080235792278No Hit
AAGCTGCCAGTTGAAGAACTGT3691373.3566645573297853No Hit
TTCAAGTAATCCAGGATAGGCT3143792.8587349598354557No Hit
TAGCAGCACGTAAATATTGGCG3107962.8261537525630533No Hit
CCCCCACTGCTAAATTTGACTG3105962.824335097398532No Hit
AACATTCAACGCTGTCGGTGAG2272072.066055919827133No Hit
TAGCTTATCAGACTGATGTTGAC2119731.9275289559455338No Hit
TAGCTTATCAGACTGATGTTGA1795821.6329886587754614No Hit
TAGCTTATCAGACTGATGTTGACT1619191.472374127920749No Hit
CGCCTGTCTGAGCGTCGCT1333861.2129156888742951No Hit
TGAGGTAGTAGGTTGTATAGTT1316101.1967660310133446No Hit
GCCTGTCTGAGCGTCGCT1215501.1052876762379154No Hit
TATTGCACTTGTCCCGGCCTGTAA1190301.082372621164945No Hit
TATTGCACTTGTCCCGGCCTGT1044340.9496471672581691No Hit
TATTGCACTTGTCCCGGCCTGTAT1030330.9369074878306963No Hit
CATAAAGTAGAAAGCACTACT945840.8600784004054873No Hit
TGTAAACATCCTTGACTGGAAGCT871420.7924062417336439No Hit
AGCAGCATTGTACAGGGCTATGA784810.7136493798340422No Hit
TGAGGTAGTAGATTGTATAGTT782000.7110941693278896No Hit
ACTGGACTTGGAGTCAGAAGGC668460.6078491156380065No Hit
TATTGCACTTGTCCCGGCCTGTA612960.5573814348225361No Hit
TTCAAGTAATTCAGGATAGGTT552710.5025944479913272No Hit
TGTAAACATCCCCGACTGGAAGCT546370.49682931111979417No Hit
TCGTACGACTCTTAGCGG531860.48363496790119104No Hit
TCCTGTCTGAGCGTCGCT478820.435404232938082No Hit
CAACGGAATCCCAAAAGCAGCTGT426640.3879555196957172No Hit
CCCATAAAGTAGAAAGCACTAC424860.38633691659929303No Hit
CCCATAAAGTAGAAAGCACT398490.36235794825507767No Hit
CATTGCACTTGTCTCGGTCTGA393920.35820232120414613No Hit
TTAATGCTAATCGTGATAGGGGTT392360.3567837701758194No Hit
TGAGGTAGTAGTTTGTACAGTT333180.30296976385762947No Hit
TACGCCTGTCTGAGCGTCGCT330540.3005691390404612No Hit
AACATTCAACGCTGTCGGTGAGTT328770.2989596292198597No Hit
CAACACCAGTCGATGGGCTGT322660.29340363769224664No Hit
CTACGCCTGTCTGAGCGTCGCT318850.28993909960383324No Hit
TTCTCACTACTGCACTTGACTAG316200.28752938151084234No Hit
ACGCCTGTCTGAGCGTCGCT279820.254448044068197No Hit
TGTAAACATCCTACACTCTCAGCT273420.24862834754172838No Hit
CTCCCACTGCTTCACTTGACTAG271830.2471825166859338No Hit
TGATGTAGTAGGTTGTATAGTT266740.24255403929222671No Hit
CAACGGAATCCCAAAAGCAGCT262930.2390895012038133No Hit
GCGTTCGCGCTTTCCCCTG261220.23753455103814747No Hit
CTTCTCACTACTGCACTTGACTAG260840.23718900655688838No Hit
GGAATACCGGGTGCTGTAGGCTTT248740.22618614281153357No Hit
TTATAAAGCAATGAGACTGATT227900.20723575599722No Hit
CCCCCCACTGCTAAATTTGACTGG221750.20164339136631654No Hit
CACCCGGCTGTGTGCACATGTGC216580.19694216776602855No Hit
TGAGGTAGTAGTTTGTGCTGTT213450.19409597243355248No Hit
TTCAAGTAATCCAGGATAGGCTT210010.1909678855505756No Hit
TAGCTTATCAGACTGATGTTGACA210000.19095879227475296No Hit
GGAATACCGGGTGCTGTAGGCTT206380.1876670264269691No Hit
CCCCCACTGCTAAATTTGACTGGCTT192650.17518195872252934No Hit
ACCGGGTGCTGTAGGCTT185360.1685529606478486No Hit
ACCACAGGGTAGAACCACGGAC184160.16746176754913575No Hit
CCCCCCACTGCTAAATTTGACTGGCT172300.1566771424235235No Hit
AACGGAATCCCAAAAGCAGCTG171820.15624066518403834No Hit
TATTGCACTTGTCCCGGCCTGTT170280.15484030070735683No Hit
AGCTCGGTCTGAGGCCCCTCAGT168710.1534126564032075No Hit
TAGCAGCACGTAAATATTGGC164750.149811719177455No Hit
AACCGGGCGGAAACACCA164290.14939342848961507No Hit
CAAAGAATTCTCCTTTTGGGCTT163710.14886601849190387No Hit
CGCGACCTCAGATCAGACGTGG162420.1476929859107875No Hit
CAAAGAATTCTCCTTTTGGGCT159800.14531054764526438No Hit
CGCGACCTCAGATCAGACGTGGCG159700.14521961488703833No Hit
TGATGTAGTAGATTGTATAGTT158820.1444194066146489No Hit
ACCCCCCACTGCTAAATTTGACTG158030.14370103782466292No Hit
AGCAGCATTGTACAGGGCTATGT156440.14225520696886834No Hit
TGAGGTAGTAGGTTGTGTGGTT151410.13768128923009687No Hit
TGTAAACATCCCCGACTGGAAGC150420.13678105492365877No Hit
TGTAAACATCCTACACTCAGCT150040.13643551044239968No Hit
TGTCAGTTTGTCAAATACCCCAA149490.1359353802721563No Hit
ACTTGACTTGGAGTCAGAAGGC148190.13475325441521735No Hit
ACTGGACTTGGAGTCAGAAGGCA147370.13400760579776355No Hit
ACTGGACTTGGAGTCAGAAGG144650.13153423477401438No Hit
TTCAAGTAATCCAGGATAGGC141970.12909723685355562No Hit
AACATTCAACGCTGTCGGTGA141100.12830612185698878No Hit
ACTGGACTTGGAGTCAGAAGGCT138650.12607826928045No Hit
TATTGCACTTGTCCCGGCCTGTAG136070.12373220411821732No Hit
CACTAGATTGTGAGCTCCTGGA134690.1224773320546975No Hit
AGCAGCATTGTACAGGGCTATG133540.12143160533509767No Hit
CAAAGTGCTGTTCGTGCAGGT133150.12107696757801599No Hit
ATCCCGGACGAGCCCCCA130210.11840354448616945No Hit
TTCACAGTGGCTAAGTTCCG129470.11773064207529652No Hit
ATCCCACCGCTGCCACCA129100.11739419086986003No Hit
TGTCAGTTTGTCAAATACCCCA125890.1144752493308031No Hit
TTCACAGTGGCTAAGTTCTGC123400.11221102365097388No Hit
TGAGGTAGTAGGTTGTATAGT122460.11135625572364881No Hit
AACATTCAACGCTGTCGGTGAGA119770.10891016452736745No Hit
TAGCAGCACGTAAATATTGGCGT118840.10806448987586498No Hit
ACCGGGTGCTGTAGGCTTT117990.10729156143094334No Hit
CTCCCACTGCTTCACTTGACTAGCCT114550.10416347454796644No Hit
CCTCGACACAAGGGTTTGT112200.10202655472965373No Hit
GACCCGCGGGCGCTCTCCAGTCCT111500.10139002542207123No Hit
ACCGGGTGCTGTAGGCTTA110740.10069893645955307No Hit
CAAAGTGCTGTTCGTGCAGGTAG110540.10051707094310093No Hit
TCGTTCGCGCTTTCCCCTG110340.10033520542664877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)