FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8113106
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG86656110.681001825934482No Hit
CAACGGAATCCCAAAAGCAGCTG6170677.605804731258288No Hit
CCCCCACTGCTAAATTTGACTG3287204.051715828685093No Hit
AACATTCAACGCTGTCGGTGAGT2431352.996817741565314No Hit
AACATTCAACGCTGTCGGTGAG1963492.42014587261648No Hit
TTCAAGTAATCCAGGATAGGCT1640302.0217904215722067No Hit
TATTGCACTTGTCCCGGCCTGTAA1455191.7936287286274826No Hit
TAGCAGCACGTAAATATTGGCG1407261.7345514775722146No Hit
TATTGCACTTGTCCCGGCCTGTAT1242261.5311768390552274No Hit
AAGCTGCCAGTTGAAGAACTGT1236831.5244839645876684No Hit
TAGCTTATCAGACTGATGTTGAC1230131.516225721690312No Hit
TCGTACGACTCTTAGCGG1188611.4650492671980373No Hit
TAGCTTATCAGACTGATGTTGA1157751.427012046927527No Hit
TAGCTTATCAGACTGATGTTGACT1124181.3856345522910707No Hit
TGAGGTAGTAGGTTGTATAGTT1000601.2333131109096813No Hit
GCCTGTCTGAGCGTCGCT887911.0944143956704127No Hit
TATTGCACTTGTCCCGGCCTGT866181.067630572064509No Hit
GGAATACCGGGTGCTGTAGGCTTT809260.9974724846439822No Hit
TATTGCACTTGTCCCGGCCTGTA660260.8138190231953089No Hit
GGAATACCGGGTGCTGTAGGCTT642030.7913492070730989No Hit
TGAGGTAGTAGATTGTATAGTT507280.6252599189508925No Hit
TCCTGTCTGAGCGTCGCT496620.6121206847291284No Hit
TGAATACCGGGTGCTGTAGGCTTT455820.5618316832049279No Hit
ACTGGACTTGGAGTCAGAAGGC450410.5551634602087043No Hit
AGCAGCATTGTACAGGGCTATGA444490.5478666246934282No Hit
CATTGCACTTGTCTCGGTCTGA381060.4696844833532312No Hit
CATAAAGTAGAAAGCACTACT371190.457518982249215No Hit
TGAATACCGGGTGCTGTAGGCTT359020.44251856194162875No Hit
TTAATGCTAATCGTGATAGGGGTT334590.4124067897054469No Hit
TTCAAGTAATTCAGGATAGGTT310240.38239362335460675No Hit
CAACACCAGTCGATGGGCTGT304810.3757007488870477No Hit
TGTAAACATCCTTGACTGGAAGCT302630.37301373851148995No Hit
TGTAAACATCCCCGACTGGAAGCT301840.37204000539374193No Hit
CACCCGGCTGTGTGCACATGTGC292100.36003473885340587No Hit
CAACGGAATCCCAAAAGCAGCT285470.35186277610572325No Hit
CAACGGAATCCCAAAAGCAGCTGT270370.333250915247502No Hit
CTCCCACTGCTTCACTTGACTAG214230.2640542352090556No Hit
TGATGTAGTAGGTTGTATAGTT212390.2617862998461995No Hit
CGCCTGTCTGAGCGTCGCT193100.23800995574321354No Hit
CCCATAAAGTAGAAAGCACT187520.23113219524063902No Hit
TATTGCACTTGTCCCGGCCTGTAG180630.22263976336559635No Hit
ACCCCCCACTGCTAAATTTGACTG180470.22244255159491322No Hit
CACTAGATTGTGAGCTCCTGGA179130.22079090301544194No Hit
TTCTCACTACTGCACTTGACTAG174750.21539223079299097No Hit
CCCCCACTGCTAAATTTGACTGGCTT169000.2083049327840657No Hit
ACCGGGTGCTGTAGGCTT156970.19347707277582715No Hit
TAGCTTATCAGACTGATGTTGACA156690.19313195217713167No Hit
CCCATAAAGTAGAAAGCACTAC156530.19293474040644853No Hit
TATTGCACTTGTCCCGGCCTGTT154910.19093797122828174No Hit
CTTCTCACTACTGCACTTGACTAG154870.19088866828561096No Hit
AACATTCAACGCTGTCGGTGAGTT154750.1907407594575986No Hit
TGAGGTAGTAGTTTGTACAGTT154330.19022307855955536No Hit
CCCCCCACTGCTAAATTTGACTGG148610.18317275775763314No Hit
AACGGAATCCCAAAAGCAGCTG141260.17411334204187645No Hit
CACTAGATTGTGAGCTCCTGGAA140650.17336147216614697No Hit
TGAGGTAGTAGTTTGTGCTGTT136380.1680983830360407No Hit
GGAATACCGGGTGCTGTAGGCTTA124730.1537389009831746No Hit
TCGTTTCCCGGCCAATGCACCA124190.15307331125711904No Hit
ACTGGACTTGGAGTCAGAAGGCA121510.14977001409817645No Hit
CGCGACCTCAGATCAGACGTGG120830.14893186407277312No Hit
TAGCAGCACGTAAATATTGGC118930.14658997429591084No Hit
ATCCCGGACGAGCCCCCA118660.14625717943288302No Hit
CGCCCCACTGCTAAATTTGACTG117650.14501228013044573No Hit
ACCGGGTGCTGTAGGCTTT115490.14234992122622336No Hit
GGAATACCGGGTGCTGTAGGCT112260.13836870860555747No Hit
AACCGGGCGGAAACACCA111240.13711148356745248No Hit
TGATGTAGTAGATTGTATAGTT110480.13617472765670757No Hit
TATTGCACTTGTCCCGGCCTGTAAT110070.13566937249433203No Hit
ACTGGACTTGGAGTCAGAAGGCT109340.1347695937905902No Hit
CCCCCCACTGCTAAATTTGACTGT107650.13268654446274955No Hit
TTATAAAGCAATGAGACTGATT107530.13253863563473717No Hit
ACCGGGTGCTGTAGGCTTA104020.12821230241537582No Hit
CTCCCACTGCTTCACTTGACTAGCCT103960.12813834800136964No Hit
TTAGGTAGTAGGTTGTATAGTT102990.1269427516416031No Hit
ACTTGACTTGGAGTCAGAAGGC100470.12383666625334366No Hit
CAAAGAATTCTCCTTTTGGGCT99920.12315875079162038No Hit
ACTGGACTTGGAGTCAGAAGG99280.12236990370888781No Hit
CCCCCCACTGCTAAATTTGACTGTA98740.12170431398283223No Hit
TCCTGTACTGAGCTGCCCCGAG98120.12094011837143505No Hit
TGAGGTAGTAGGTTGTGTGGTT96840.11936242420596994No Hit
TTCACAGTGGCTAAGTTCTG96550.11900497787160677No Hit
TACCACCCTGAACGCGCCCGA96290.11868450874424666No Hit
CCCCCCACTGCTAAATTTGACTGGCT95410.1175998440054894No Hit
TTCAAGTAATCCAGGATAGGCTT91780.11312560195811568No Hit
CTCCCCACTGCTAAATTTGACTG87060.10730785472296306No Hit
ACCACAGGGTAGAACCACGGAC87020.10725855178029227No Hit
AGCTCGGTCTGAGGCCCCTCAGT86550.10667924220391058No Hit
TGAGAACTGAATTCCATGGGTT86120.10614923557019963No Hit
TCCTGTACTGAGCTGCCCCGAGT85770.10571783482183025No Hit
TATTGCACTTGTCCCGGCCTGTAAA85530.10542201716580554No Hit
CTACGCCTGTCTGAGCGTCGCT85190.10500294215310389No Hit
CGCGACCTCAGATCAGACGTGGCG84430.10406618624235896No Hit
TGAGGTAGTAGGTTGTATAGT84280.10388130020734351No Hit
GACCCGCGGGCGCTCTCCAGTCCT81510.10046707142739167No Hit
AGCAGCATTGTACAGGGCTATGT81220.1001096250930285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)