FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8772139
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAATACCGGGTGCTGTAGGCTTT124654414.210262742074653No Hit
GGAATACCGGGTGCTGTAGGCTT98295811.20545399474404No Hit
TGAATACCGGGTGCTGTAGGCTTT6749967.694770910492868No Hit
CCCCCCACTGCTAAATTTGACTG5584706.366406186678073No Hit
TGAATACCGGGTGCTGTAGGCTT5294816.03593946698747No Hit
CAACGGAATCCCAAAAGCAGCTG2825243.220696799264125No Hit
GGAATACCGGGTGCTGTAGGCTTA2189422.4958792832626115No Hit
GGAATACCGGGTGCTGTAGGCT1680851.9161233081235944No Hit
CCCCCACTGCTAAATTTGACTG1608991.8342048615508713No Hit
AACATTCAACGCTGTCGGTGAGT1217151.3875179132478406No Hit
TGAATACCGGGTGCTGTAGGCTTA1195561.362905900145905No Hit
AACATTCAACGCTGTCGGTGAG952441.0857557090693617No Hit
TTCAAGTAATCCAGGATAGGCT929771.0599125253259212No Hit
TGAATACCGGGTGCTGTAGGCT912551.0402821934308155No Hit
TAGCAGCACGTAAATATTGGCG856720.9766375111019102No Hit
TATTGCACTTGTCCCGGCCTGTAT806770.9196958689323095No Hit
AAGCTGCCAGTTGAAGAACTGT722320.8234251646035249No Hit
TATTGCACTTGTCCCGGCCTGTAA719540.820256040174466No Hit
TATTGCACTTGTCCCGGCCTGT501350.5715253714059935No Hit
GCCTGTCTGAGCGTCGCT491460.5602510402536941No Hit
TAGCTTATCAGACTGATGTTGACT491440.5602282407973699No Hit
TAGCTTATCAGACTGATGTTGAC446860.5094082526508072No Hit
TAGCTTATCAGACTGATGTTGA431170.49152207916450025No Hit
GGAATACCGGGTGCTGTAGGCTA382980.4365867891514259No Hit
TATTGCACTTGTCCCGGCCTGTA379730.4328818774987492No Hit
TGAGGTAGTAGGTTGTATAGTT363300.41415212412844804No Hit
CGAATACCGGGTGCTGTAGGCTTT326150.3718021340063125No Hit
TTAATACCGGGTGCTGTAGGCTTT292210.3331114566242053No Hit
CGAATACCGGGTGCTGTAGGCTT263300.3001548425076256No Hit
TGAGGTAGTAGATTGTATAGTT247670.282337067390291No Hit
ATCCCGGACGAGCCCCCA241500.2753034351142863No Hit
TCCTGTCTGAGCGTCGCT240700.2743914568613197No Hit
CTCCCACTGCTTCACTTGACTAG223540.25482952333518655No Hit
TTAATACCGGGTGCTGTAGGCTT221640.25266357498439096No Hit
AGCAGCATTGTACAGGGCTATGA210360.23980468161756216No Hit
TGAATACCGGGTGCTGTAGGCTA206090.23493699769235304No Hit
TGTAAACATCCCCGACTGGAAGCT201800.23004651431081974No Hit
TGTAAACATCCTTGACTGGAAGCT199770.22773236949391704No Hit
ACTGGACTTGGAGTCAGAAGGC194410.221622115199041No Hit
TTCAAGTAATTCAGGATAGGTT192510.21945616684824532No Hit
ATACCGGGTGCTGTAGGCTTA176470.2011710028762654No Hit
GTAATACCGGGTGCTGTAGGCTTT175470.20003103006005718No Hit
CATAAAGTAGAAAGCACTACT172430.1965655126987842No Hit
ATACCGGGTGCTGTAGGCTT166540.18985107281131774No Hit
ATACCGGGTGCTGTAGGCTTT165730.1889276948301891No Hit
TCGTACGACTCTTAGCGG153870.17540761722995954No Hit
TTCTCACTACTGCACTTGACTAG149640.17058553221739875No Hit
CAACGGAATCCCAAAAGCAGCTGT145700.16609403932153832No Hit
CATTGCACTTGTCTCGGTCTGA137990.15730484890857294No Hit
AGAATACCGGGTGCTGTAGGCTTT135000.15389633018811033No Hit
GAATACCGGGTGCTGTAGGCTTT134280.1530755497604404No Hit
GTAATACCGGGTGCTGTAGGCTT131710.15014581962278528No Hit
CCCCCACTGCTAAATTTGACTGGCTT131710.15014581962278528No Hit
CTTCTCACTACTGCACTTGACTAG126870.14462835119233747No Hit
CCCCCCACTGCTAAATTTGACTGGCT118260.13481318524478464No Hit
CACCCGGCTGTGTGCACATGTGC117260.13367321242857644No Hit
ACCCCCCACTGCTAAATTTGACTG111180.1267421777060304No Hit
TGATGTAGTAGGTTGTATAGTT111080.1266281804244096No Hit
AATACCGGGTGCTGTAGGCTT110920.12644578477381627No Hit
CCCCCCACTGCTAAATTTGACTGG110520.12598979564733298No Hit
AGAATACCGGGTGCTGTAGGCTT109090.12435963452015522No Hit
AGCTCGGTCTGAGGCCCCTCAGT107660.12272947339297746No Hit
TATTGCACTTGTCCCGGCCTGTT106640.1215667011204451No Hit
TATTGCACTTGTCCCGGCCTGTAG106070.12091691661520639No Hit
CGCCTGTCTGAGCGTCGCT105280.12001633809040191No Hit
AATACCGGGTGCTGTAGGCTTA102340.11666481801074972No Hit
CACTAGATTGTGAGCTCCTGGA101460.11566164193248649No Hit
CCCATAAAGTAGAAAGCACTAC100030.11403148080530873No Hit
CCCATAAAGTAGAAAGCACT97580.11123854740559856No Hit
GAATACCGGGTGCTGTAGGCTT97370.11099915311419484No Hit
AACCGGGCGGAAACACCA97170.11077115855095318No Hit
CACTAGATTGTGAGCTCCTGGAA94620.10786422786962223No Hit
CTCCCACTGCTTCACTTGACTAGCCT92290.10520809120785705No Hit
AATACCGGGTGCTGTAGGCTTT87990.1003062080981617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)