FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8332975
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG196745923.610523252499856No Hit
CCCCCCACTGCTAAATTTGACTG4567165.48082767559005No Hit
TAGCTTATCAGACTGATGTTGAC4245075.094303055031366No Hit
TAGCAGCACGTAAATATTGGCG3303713.964622478766587No Hit
CCCCCACTGCTAAATTTGACTG2314362.7773514261113226No Hit
AAGCTGCCAGTTGAAGAACTGT2112432.5350250060752613No Hit
TTCAAGTAATCCAGGATAGGCT1921272.305623141795097No Hit
AACATTCAACGCTGTCGGTGAG1734902.081969524689562No Hit
AACATTCAACGCTGTCGGTGAGT1668182.0019020817895172No Hit
TAGCTTATCAGACTGATGTTGA1480971.7772404213381177No Hit
TGAGGTAGTAGGTTGTATAGTT1476731.7721522025447096No Hit
TAGCTTATCAGACTGATGTTGACT1223861.4686951538916173No Hit
AGCAGCATTGTACAGGGCTATGA746020.8952624962873403No Hit
CATAAAGTAGAAAGCACTACT699230.8391120818195182No Hit
TCGTACGACTCTTAGCGG659780.7917700461119828No Hit
TAGCTTATCAGACTGATGTTGACA653070.783717699861094No Hit
TATTGCACTTGTCCCGGCCTGT534960.6419796051230202No Hit
TTCACAGTGGCTAAGTTCCGC515860.6190586195206395No Hit
TGTAAACATCCTTGACTGGAAGCT476230.5715005745247046No Hit
CATTGCACTTGTCTCGGTCTGA409510.49143313162465985No Hit
ACTGGACTTGGAGTCAGAAGGC396310.4755924504753704No Hit
TTCAAGTAATTCAGGATAGGTT386020.463243919488538No Hit
TATTGCACTTGTCCCGGCCTGTA379160.45501156549731636No Hit
TGAGGTAGTAGATTGTATAGTT375360.45045136940888464No Hit
TAGCAGCACGTAAATATTGGC369280.4431550556673937No Hit
CACCCGGCTGTGTGCACATGTGC364780.4377548234574087No Hit
CCCCCACTGCTAAATTTGACTGGCTT342150.4105976556991951No Hit
AACGGAATCCCAAAAGCAGCTG333020.39964118457093656No Hit
CAACGGAATCCCAAAAGCAGCT289310.3471869290379486No Hit
TGTAAACATCCCCGACTGGAAGCT267470.3209778020454879No Hit
CTCCCACTGCTTCACTTGACTAGCCT264660.31760565704325283No Hit
TGAGGTAGTAGTTTGTGCTGTT256570.307897239581302No Hit
CAACGGAATCCCAAAAGCAGCTGT236220.2834761894761475No Hit
TATTGCACTTGTCCCGGCCTGTAT234930.2819281229092851No Hit
CGCGACCTCAGATCAGACGTGG224490.26939958418211984No Hit
AATCCTTGGAACCTAGGTGTGAGT220850.2650313963500431No Hit
TAGCAGCACGTAAATATTGGCGT216400.25969116672016895No Hit
TGTAAACATCCTACACTCTCAGCT215800.2589711357588376No Hit
TCAGTGCATCACAGAACTTTGT214960.25796309241297377No Hit
TGATGTAGTAGGTTGTATAGTT201990.2423984231321947No Hit
TATTGCACTTGTCCCGGCCTGTAA196050.23527011661501446No Hit
ACCCCCCACTGCTAAATTTGACTG194890.23387805675644052No Hit
CCCATAAAGTAGAAAGCACTAC191580.229905885953096No Hit
AACATTCAACGCTGTCGGTGAGTT177050.21246913617285543No Hit
TGAGGTAGTAGGTTGTGTGGTT170110.20414077805345632No Hit
TTCACAGTGGCTAAGTTCTGC159870.1918522496467348No Hit
CACTAGATTGTGAGCTCCTGGA158500.19020817895169492No Hit
CACTAGATTGTGAGCTCCTGGAA152080.1825038476654496No Hit
CAAAGAATTCTCCTTTTGGGCT151150.18138779967538604No Hit
TTCACCACCTTCTCCACCCAGC146350.17562755198473534No Hit
AGCAGCATTGTACAGGGCTATG142820.17139136982890266No Hit
AGCAGCATTGTACAGGGCTATGT140950.1691472733327533No Hit
TCAGTGCATCACAGAACTTTGTT140450.16854724753164388No Hit
ATCACATTGCCAGGGATTTCC137310.16477908550067655No Hit
CAAAGAATTCTCCTTTTGGGCTT127880.15346259889175234No Hit
CCCATAAAGTAGAAAGCACT125870.15105049517129238No Hit
GTGCGGTAACGCGACCGATCCCGGA124710.14965843531271844No Hit
TGAGGTAGTAAGTTGTATTGTT123720.14847038422652173No Hit
TCAGTGCACTACAGAACTTTGT121760.1461182830861727No Hit
TGAGAACTGAATTCCATAGGCTGT116940.14033403436347763No Hit
CAAAGTGCTGTTCGTGCAGGTAG114430.1373219048419082No Hit
CCCCCCACTGCTAAATTTGACTGG111630.1339617603556953No Hit
CGCGACCTCAGATCAGACGTG109370.1312496437346806No Hit
TTATAAAGCAATGAGACTGATT108690.13043360864517176No Hit
CCCATAAAGTAGAAAGCACTA106950.12834551885731088No Hit
CAAAGAATTCTCCTTTTGGGC106830.1282015126650446No Hit
TATTGCACTTGTCCCGGCCTGTT100990.12119321130808626No Hit
ACTGGACTTGGAGTCAGAAGG98350.11802507507822836No Hit
TGAGAACTGAATTCCATGGGTT98000.11760505701745175No Hit
TGAGGTAGTAGTTTGTACAGTT95520.11462892904394889No Hit
TAGCAGCACATAATGGTTTGTG94810.11377689240637348No Hit
TCCCACTGCTTCACTTGACTAGCCT94120.11294885680084242No Hit
TGGCTCAGTTCAGCAGGAACAGT93020.11162880003840166No Hit
TCAGTGCACTACAGAACTTTGTC92840.11141279075000225No Hit
CTTCTCACTACTGCACTTGACTAG91890.1102727417278943No Hit
TGTAAACATCCCCGACTGGAAGC84710.10165637122396262No Hit
TAGCTTATCAGACTGATGTTGACC84480.10138035935545228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)