FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7511379
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT91923312.237872699540258No Hit
CAACGGAATCCCAAAAGCAGCTG5310297.069660577638275No Hit
CCCCCCACTGCTAAATTTGACTG3806255.0673118744241235No Hit
TTCAAGTAATCCAGGATAGGCT2706933.603772356580596No Hit
AAGCTGCCAGTTGAAGAACTGT2445313.2554741279863526No Hit
TAGCAGCACGTAAATATTGGCG2158512.873653426354868No Hit
CCCCCACTGCTAAATTTGACTG1801282.398068317415484No Hit
AACATTCAACGCTGTCGGTGAGT1291251.7190585110936356No Hit
TGAGGTAGTAGGTTGTATAGTT1073721.4294578931511777No Hit
AACATTCAACGCTGTCGGTGAG1012471.347914943447801No Hit
CATAAAGTAGAAAGCACTACT932331.2412234823991706No Hit
TATTGCACTTGTCCCGGCCTGT855201.1385392748788203No Hit
TAGCTTATCAGACTGATGTTGAC760871.0129564757682976No Hit
TATTGCACTTGTCCCGGCCTGTAA714610.9513699148984494No Hit
TATTGCACTTGTCCCGGCCTGTAT671040.8933645872482269No Hit
TCCTGTCTGAGCGTCGCT623760.8304200866445429No Hit
TAGCTTATCAGACTGATGTTGACT619870.8252412772674631No Hit
AACCGGGCGGAAACACCA598780.7971638763002107No Hit
TGAGGTAGTAGATTGTATAGTT568570.7569448965363084No Hit
TAGCTTATCAGACTGATGTTGA565500.7528577642001555No Hit
TGTAAACATCCTTGACTGGAAGCT468960.6243327623329884No Hit
TTCAAGTAATTCAGGATAGGTT465230.6193669631102359No Hit
TATTGCACTTGTCCCGGCCTGTA397460.5291438496180262No Hit
CCCATAAAGTAGAAAGCACTAC386010.5139003104489868No Hit
GGAATACCGGGTGCTGTAGGCTTT356570.4747064420527842No Hit
CAACGGAATCCCAAAAGCAGCTGT335530.44669560675875897No Hit
CGCCTGTCTGAGCGTCGCT335410.44653584914301353No Hit
CACCCGGCTGTGTGCACATGTGC320640.42687234927168505No Hit
CATTGCACTTGTCTCGGTCTGA310570.41346602268371757No Hit
AGCAGCATTGTACAGGGCTATGA307560.4094587691554374No Hit
GGAATACCGGGTGCTGTAGGCTT281250.3744319119032604No Hit
TGAGAACTGAATTCCATGGGTT265340.3532507146823506No Hit
CTACGCCTGTCTGAGCGTCGCT253120.3369820641456116No Hit
TGTAAACATCCCCGACTGGAAGCT246420.3280622639331606No Hit
ACTGGACTTGGAGTCAGAAGGC244010.32485379848360735No Hit
TGAGGTAGTAGTTTGTACAGTT241050.3209131106285544No Hit
TTCACAGTGGCTAAGTTCCGC235260.31320480566883924No Hit
CAACACCAGTCGATGGGCTGT234810.312605714609794No Hit
TTCTCACTACTGCACTTGACTAG215360.2867116677243952No Hit
CCCATAAAGTAGAAAGCACT212170.2824647777724969No Hit
TTAATGCTAATCGTGATAGGGGTT199030.26497131884837655No Hit
ATCCCGGACGAGCCCCCA183210.24390993983927586No Hit
TGATGTAGTAGGTTGTATAGTT182050.24236561622040373No Hit
ACCGGGTGCTGTAGGCTT178940.23822523134566903No Hit
CTCCCACTGCTTCACTTGACTAG169350.2254579352206832No Hit
TGAGGTAGTAGTTTGTGCTGTT151960.20230639407224693No Hit
TAGCAGCACGTAAATATTGGC150990.20101502001163835No Hit
TCGTACGACTCTTAGCGG144820.19280081593539616No Hit
ACCGGGTGCTGTAGGCTTT143460.19099022962361503No Hit
ACCGGGTGCTGTAGGCTTA141260.1880613400016162No Hit
TGTAAACATCCTACACTCTCAGCT139750.18605105667015337No Hit
AACGGAATCCCAAAAGCAGCTG138830.18482624828277205No Hit
TTCAAGTAATCCAGGATAGGC137520.1830822276442182No Hit
TACGCCTGTCTGAGCGTCGCT132380.17623927643645726No Hit
CGCGACCTCAGATCAGACGTGG128410.1709539619822139No Hit
AGCTCGGTCTGAGGCCCCTCAGT125160.1666271932224429No Hit
ACGCCTGTCTGAGCGTCGCT122130.1625933134248718No Hit
TGAGAACTGAATTCCATAGGCTGT118030.15713492822023759No Hit
TTCACAGTGGCTAAGTTCTGC116060.15451224069508407No Hit
TATTGCACTTGTCCCGGCCTGTT115290.15348712932738448No Hit
ACCCCCCACTGCTAAATTTGACTG114800.15283478572975748No Hit
CCCCCACTGCTAAATTTGACTGGCTT114550.15250195736362124No Hit
TTATAAAGCAATGAGACTGATT111380.14828169368101382No Hit
CGCGACCTCAGATCAGACGTGGCG109440.14569894555979668No Hit
TGAGGTAGTAGGTTGTGTGGTT108130.14395492492124282No Hit
CCCCCCACTGCTAAATTTGACTGG107580.14322270251574312No Hit
TTCAAGTAATCCAGGATAGGCTT107080.14255704578347064No Hit
CTTCTCACTACTGCACTTGACTAG104930.13969472183469905No Hit
TACCACCCTGAACGCGCCCGA101270.13482211455446463No Hit
TGAGGTAGTAGGTTGTATAGT101130.13463573066942836No Hit
TTCACAGTGGCTAAGTTCCG100350.1335973061670833No Hit
TAGCAGCACATCATGGTTTACA97560.12988294160100297No Hit
TGATGTAGTAGATTGTATAGTT97490.12978974965848483No Hit
TGTCAGTTTGTCAAATACCCCAA92390.1230000509893057No Hit
TGTCAGTTTGTCAAATACCCCA90800.12088326258067926No Hit
ACCACAGGGTAGAACCACGGAC88720.11811413057442582No Hit
CACTAGATTGTGAGCTCCTGGA88650.11802093863190767No Hit
CAAAGAATTCTCCTTTTGGGCT86630.11533168543352691No Hit
AGAGGTAGTAGGTTGCATAGTT86150.11469265497054536No Hit
CAACGGAATCCCAAAAGCAGCT85900.11435982660440913No Hit
ATCCCACCGCTGCCACCA85720.11412019018079103No Hit
TGTAAACATCCTACACTCAGCT84650.11269568477372796No Hit
TATTGCACTTGTCCCGGCCTGTAG83610.11131111877060124No Hit
TCCCACTGCTTCACTTGACTAG82690.1100863103832199No Hit
AACATTCAACGCTGTCGGTGAGTT82520.10985998709424727No Hit
TAGCAGCACGTAAATATTGGCGT81010.10784970376278442No Hit
ATCACATTGCCAGGGATTTCC80890.10768994614703904No Hit
CCCTGTCTGAGCGTCGCT79580.1059459255084852No Hit
TCAGTGCACTACAGAACTTTGT79150.10537346071873087No Hit
TAGCTTATCAGACTGATGTTGACA78880.10501400608330373No Hit
TGTAAACATCCCCGACTGGAAGC78880.10501400608330373No Hit
CCCATAAAGTAGAAAGCACTA75720.10080705553534178No Hit
GCCTGTCTGAGCGTCGCG75220.10014139880306931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)