FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6604736
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG85564112.954961409509783No Hit
CAACGGAATCCCAAAAGCAGCTG5700318.63064019515693No Hit
CCCCCACTGCTAAATTTGACTG2796784.234506875066619No Hit
TTCAAGTAATCCAGGATAGGCT2204263.337393046444249No Hit
TAGCAGCACGTAAATATTGGCG1950152.9526539743602167No Hit
AAGCTGCCAGTTGAAGAACTGT1903232.881614041802731No Hit
AACATTCAACGCTGTCGGTGAGT1609072.436236664114962No Hit
TATTGCACTTGTCCCGGCCTGTAA1130371.7114537204817877No Hit
AACATTCAACGCTGTCGGTGAG1058261.6022744890938867No Hit
TAGCTTATCAGACTGATGTTGACT1007501.525420546710724No Hit
TATTGCACTTGTCCCGGCCTGTAT994711.5060556546090562No Hit
TAGCTTATCAGACTGATGTTGAC969241.4674924175621857No Hit
GCCTGTCTGAGCGTCGCT764831.1580023788990204No Hit
TGAGGTAGTAGGTTGTATAGTT711161.076742507194837No Hit
TGTAAACATCCTTGACTGGAAGCT609200.9223684338026531No Hit
TATTGCACTTGTCCCGGCCTGT551330.8347494888516361No Hit
TAGCTTATCAGACTGATGTTGA513490.7774572670277813No Hit
AACCGGGCGGAAACACCA432670.655090528977994No Hit
TGAGGTAGTAGATTGTATAGTT411390.6228712245273694No Hit
CAACGGAATCCCAAAAGCAGCT409460.6199490789639435No Hit
CAACGGAATCCCAAAAGCAGCTGT397180.6013563600422485No Hit
TATTGCACTTGTCCCGGCCTGTA392600.5944219420730822No Hit
TTCAAGTAATTCAGGATAGGTT388990.5889561672109226No Hit
AGCAGCATTGTACAGGGCTATGA379660.5748299402125989No Hit
TGTAAACATCCCCGACTGGAAGCT376900.5706511206503939No Hit
TTAATGCTAATCGTGATAGGGGTT370010.5602192123954689No Hit
CACCCGGCTGTGTGCACATGTGC330260.5000351263093634No Hit
CATAAAGTAGAAAGCACTACT322310.4879983090921423No Hit
ACTGGACTTGGAGTCAGAAGGC305910.4631676421283152No Hit
CCCATAAAGTAGAAAGCACTAC301350.4562635054603242No Hit
CCCCCACTGCTAAATTTGACTGGCTT297330.450176963924069No Hit
TGAGAACTGAATTCCATAGGCTGT270780.4099785366137269No Hit
TGAGAACTGAATTCCATGGGTT239830.36311822304479696No Hit
TTCTCACTACTGCACTTGACTAG233820.3540186920415896No Hit
ACCCCCCACTGCTAAATTTGACTG221540.3354259731198946No Hit
CAAAGAATTCTCCTTTTGGGCT188590.28553752943342475No Hit
TCGTTTCCCGGCCAATGCACCA179260.2714113024351011No Hit
TGAGGTAGTAGTTTGTACAGTT177500.268746547931666No Hit
CATTGCACTTGTCTCGGTCTGA176480.26720220157172064No Hit
TGTAAACATCCTACACTCTCAGCT172510.2611913632884039No Hit
CACCCGTAGAACCGACCTTGCG160230.24259864436670897No Hit
TCCTGTCTGAGCGTCGCT160150.24247751916200735No Hit
CCCCCCACTGCTAAATTTGACTGG159620.24167506468085934No Hit
CTTCTCACTACTGCACTTGACTAG155620.2356188044457795No Hit
AGCTCGGTCTGAGGCCCCTCAGT155190.23496775647050844No Hit
CTCCCACTGCTTCACTTGACTAG151140.22883579298249015No Hit
TAGCTTATCAGACTGATGTTGACA145050.21961513677458117No Hit
GGAATACCGGGTGCTGTAGGCTTT142100.2151486448512098No Hit
CAAAGAATTCTCCTTTTGGGCTT137100.20757831955736006No Hit
CACTAGATTGTGAGCTCCTGGA132340.20037136987761509No Hit
TATTGCACTTGTCCCGGCCTGTAG131780.1995234934447039No Hit
TAGCAGCACGTAAATATTGGC129600.19622283161658544No Hit
CACTAGATTGTGAGCTCCTGGAA128550.19463306330487698No Hit
CCCATAAAGTAGAAAGCACT128340.1943151096425353No Hit
AACGGAATCCCAAAAGCAGCTG125760.19040882179090884No Hit
CCCCCCACTGCTAAATTTGACTGGCT124200.1880468802992277No Hit
CAACGGAATCCCAAAAGCAGCTGA123020.18626028352987917No Hit
TTCAAGTAATCCAGGATAGGCTT118640.17962867857246678No Hit
CGCGACCTCAGATCAGACGTGGCG110810.16777354916229809No Hit
AACATTCAACGCTGTCGGTGAGTT109260.16542674832120466No Hit
TATTGCACTTGTCCCGGCCTGTT108600.1644274653824165No Hit
TGTAAACATCCCCGACTGGAAGC108490.1642609182259518No Hit
TGAGGTAGTAGTTTGTGCTGTT102560.155282512427446No Hit
TATTGCACTTGTCCCGGCCTGTAAT98400.14898400178296303No Hit
TATTGCACTTGTCCCGGCCTGTTT87000.13172366011298559No Hit
GGAATACCGGGTGCTGTAGGCTT86880.1315419723059332No Hit
TATTGCACTTGTCCCGGCCTGTAAA85320.12918003081425206No Hit
TTCAAGTAATCCAGGATAGGC81270.12304806732623377No Hit
CAACACCAGTCGATGGGCTGT79610.12053471932867567No Hit
TTCACAGTGGCTAAGTTCTGC79160.11985339005222918No Hit
AATGCACCCGGGCAAGGATTCT76780.1162499152123567No Hit
CTCCCACTGCTTCACTTGACTAGCCT76530.11587139894766421No Hit
TGATGTAGTAGGTTGTATAGTT76520.11585625829707653No Hit
CTACGCCTGTCTGAGCGTCGCT75920.11494781926181456No Hit
ATGCACCTGGGCAAGGATTCTGA74970.1135094574559831No Hit
ATCACATTGCCAGGGATTTCC73170.1107841403501972No Hit
TCGTACGACTCTTAGCGG72240.10937605984554113No Hit
ACTGGACTTGGAGTCAGAAGGCA71250.1078771354373589No Hit
GGGAATACCGGGTGCTGTAGGCTTT70900.10734721266678941No Hit
ACTGGACTTGGAGTCAGAAGGCT69480.10519724028333609No Hit
TTATAAAGCAATGAGACTGATT69470.10518209963274838No Hit
TTCACAGTGGCTAAGTTCCGC69420.1051063963798099No Hit
CCCACCACTGCTAAATTTGACTG68850.104243379296311No Hit
TCGTTTCCCGGGCGGCGCACCA68740.10407683213984632No Hit
TCAGTGCACTACAGAACTTTGT68640.10392542563396932No Hit
TGTCAGTTTGTCAAATACCCCA67540.10225995406932238No Hit
TGAGGTAGTAGGTTGTGTGGTT66420.10056420120350003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)