FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6661707
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG74627611.202474080592257No Hit
CAACGGAATCCCAAAAGCAGCTG5185527.784070959590387No Hit
CCCCCACTGCTAAATTTGACTG2941094.41491947934666No Hit
TTCAAGTAATCCAGGATAGGCT2512373.7713607037955885No Hit
AAGCTGCCAGTTGAAGAACTGT2364333.549135379265404No Hit
TAGCAGCACGTAAATATTGGCG1977142.9679179825831428No Hit
AACATTCAACGCTGTCGGTGAGT1446032.170659862404636No Hit
TAGCTTATCAGACTGATGTTGAC1249271.875300129531365No Hit
TGAGGTAGTAGGTTGTATAGTT1064641.5981489429060751No Hit
AACATTCAACGCTGTCGGTGAG1062641.595146709394454No Hit
TAGCTTATCAGACTGATGTTGACT966541.4508893891610664No Hit
TAGCTTATCAGACTGATGTTGA690491.036506108719582No Hit
TATTGCACTTGTCCCGGCCTGTAA678141.0179673167853225No Hit
TGTAAACATCCTTGACTGGAAGCT659340.9897463217760853No Hit
TGAGGTAGTAGATTGTATAGTT583830.8763969955448356No Hit
TATTGCACTTGTCCCGGCCTGTAT579030.8691916351169452No Hit
TATTGCACTTGTCCCGGCCTGT572280.8590590970152245No Hit
CATAAAGTAGAAAGCACTACT518360.7781188815419231No Hit
TTCAAGTAATTCAGGATAGGTT499290.7494925850086172No Hit
TTAATGCTAATCGTGATAGGGGTT492720.7396302479229423No Hit
GCCTGTCTGAGCGTCGCT468400.7031230884216313No Hit
TATTGCACTTGTCCCGGCCTGTA395280.5933614312367685No Hit
AGCAGCATTGTACAGGGCTATGA365870.5492135874483822No Hit
TGTAAACATCCCCGACTGGAAGCT314250.471725940513445No Hit
CAACGGAATCCCAAAAGCAGCTGT306420.4599721963154489No Hit
ACTGGACTTGGAGTCAGAAGGC290560.4361644845682946No Hit
CACCCGGCTGTGTGCACATGTGC262470.3939981148975781No Hit
TCGTACGACTCTTAGCGG259470.3894947646301466No Hit
CCCATAAAGTAGAAAGCACTAC253380.38035296358726073No Hit
CATTGCACTTGTCTCGGTCTGA239600.35966757469219224No Hit
CAACGGAATCCCAAAAGCAGCT233320.3502405614657024No Hit
TTCTCACTACTGCACTTGACTAG216560.3250818446383187No Hit
TCCTGTCTGAGCGTCGCT198020.29725113998559227No Hit
TGAGGTAGTAGTTTGTACAGTT190670.28621793183038524No Hit
CGCCTGTCTGAGCGTCGCT190000.2852121836039922No Hit
CAACACCAGTCGATGGGCTGT188330.2827053186217887No Hit
CCCCCACTGCTAAATTTGACTGGCTT186480.27992825262353926No Hit
ACCCCCCACTGCTAAATTTGACTG178310.2676641287285676No Hit
ACCGGGTGCTGTAGGCTT173940.26110424850567576No Hit
TGTAAACATCCTACACTCTCAGCT173800.26089409215986237No Hit
TGAGAACTGAATTCCATGGGTT162990.24466702002955099No Hit
CAAAGAATTCTCCTTTTGGGCT162870.24448688601885374No Hit
TTCACAGTGGCTAAGTTCCG162540.2439915174894363No Hit
TGAGGTAGTAGTTTGTGCTGTT150810.22638341794377928No Hit
TAGCTTATCAGACTGATGTTGACA149380.2242368209829703No Hit
CCCATAAAGTAGAAAGCACT148260.22255557021646252No Hit
TGAGAACTGAATTCCATAGGCTGT146340.21967342604530643No Hit
AACCGGGCGGAAACACCA145090.21779703010054327No Hit
CTCCCACTGCTTCACTTGACTAG143910.2160257123286869No Hit
AACGGAATCCCAAAAGCAGCTG143890.21599568999357074No Hit
CCCCCCACTGCTAAATTTGACTGG141150.21188263008265No Hit
TAGCAGCACGTAAATATTGGC131970.1981023782643097No Hit
AGCTCGGTCTGAGGCCCCTCAGT126270.18954601275618996No Hit
ACCGGGTGCTGTAGGCTTT123920.18601838838003532No Hit
CGCGACCTCAGATCAGACGTGGCG120390.18071944623202432No Hit
AACATTCAACGCTGTCGGTGAGTT118270.1775370787097061No Hit
TCGTTTCCCGGCCAATGCACCA117800.17683155383447516No Hit
CTTCTCACTACTGCACTTGACTAG117710.17669645332645223No Hit
ACCGGGTGCTGTAGGCTTA115300.17307876194494895No Hit
CACCCGTAGAACCGACCTTGCG114760.17226815889681127No Hit
TTCAAGTAATCCAGGATAGGC109150.16384689389671447No Hit
TTCAAGTAATCCAGGATAGGCTT108020.16215063196264862No Hit
CGCGACCTCAGATCAGACGTGG104360.15665654463638223No Hit
TTATAAAGCAATGAGACTGATT103120.15479515985917724No Hit
TATTGCACTTGTCCCGGCCTGTT101870.1529187639144141No Hit
CAAAGAATTCTCCTTTTGGGCTT95180.14287629281804198No Hit
CACTAGATTGTGAGCTCCTGGA94600.1420056450996719No Hit
TGAGGTAGTAGGTTGTGTGGTT94030.14115000854885992No Hit
CCCCCCACTGCTAAATTTGACTGGCT90530.13589609990352325No Hit
TGTCAGTTTGTCAAATACCCCA90280.1355208207145706No Hit
TTCACAGTGGCTAAGTTCTG88110.13226339735446185No Hit
TGAGGTAGTAGGTTGTATAGT87080.13071724709597704No Hit
TTCACAGTGGCTAAGTTCTGC84080.1262138968285456No Hit
TATTGCACTTGTCCCGGCCTGTAG83300.1250430257590134No Hit
TCAGTGCACTACAGAACTTTGT83090.12472779124029322No Hit
CACTAGATTGTGAGCTCCTGGAA80890.12142533437751014No Hit
TACGCCTGTCTGAGCGTCGCT80890.12142533437751014No Hit
AATGCACCCGGGCAAGGATTCT80630.1210350440209994No Hit
TGTCAGTTTGTCAAATACCCCAA79880.11990920645414155No Hit
CTACGCCTGTCTGAGCGTCGCT77880.11690697294252057No Hit
TGTAAACATCCCCGACTGGAAGC77670.11659173842380038No Hit
AGAGGTAGTAGGTTGCATAGTT77010.11560100136496546No Hit
CAACGGAATCCCAAAAGCAGCTGA76680.115105632835548No Hit
CAAAGTGCTGTTCGTGCAGGTAG75360.11312415871787816No Hit
TTCACAGTGGCTAAGTTCCGC71610.10749497088358884No Hit
TGAGGTAGTAAGTTGTATTGTT71150.10680445717591602No Hit
CAAAGTGCTGTTCGTGCAGGT70670.10608392113312698No Hit
CTCCCACTGCTTCACTTGACTAGCCT69650.1045527820422003No Hit
TCAGTGCACTACAGAACTTTGTC69180.10384725716696937No Hit
CCCATAAAGTAGAAAGCACTA68970.10353202264824915No Hit
AGCAGCATTGTACAGGGCTATGT67800.10177571604395089No Hit
ATCACATTGCCAGGGATTTCC67230.10092007949313891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)