FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7631268
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG84914911.127233377205465No Hit
CAACGGAATCCCAAAAGCAGCTG7209049.446713180561868No Hit
TTCAAGTAATCCAGGATAGGCT2722003.56690395357626No Hit
TAGCAGCACGTAAATATTGGCG2278402.98561130339021No Hit
CCCCCACTGCTAAATTTGACTG2109392.764140900306476No Hit
AAGCTGCCAGTTGAAGAACTGT1955372.5623133665335827No Hit
AACATTCAACGCTGTCGGTGAGT1795642.3530034589271405No Hit
AACATTCAACGCTGTCGGTGAG1406861.8435468391360388No Hit
TATTGCACTTGTCCCGGCCTGTAA965491.2651763769795532No Hit
TAGCTTATCAGACTGATGTTGAC902851.183093032507835No Hit
TATTGCACTTGTCCCGGCCTGTAT798191.0459467548512253No Hit
TGAGGTAGTAGGTTGTATAGTT797991.0456846752073181No Hit
TAGCTTATCAGACTGATGTTGACT662390.8679946766382729No Hit
TCGTACGACTCTTAGCGG612310.8023699338039236No Hit
TTCAAGTAATTCAGGATAGGTT586350.768351996024776No Hit
TAGCTTATCAGACTGATGTTGA559790.7335478193139069No Hit
TGTAAACATCCTTGACTGGAAGCT551290.7224094344478532No Hit
CAACGGAATCCCAAAAGCAGCTGT539070.7063963682051265No Hit
TATTGCACTTGTCCCGGCCTGT473590.620591492789927No Hit
CCCCCACTGCTAAATTTGACTGGCTT460970.604054267259386No Hit
TGAGGTAGTAGATTGTATAGTT460700.6037004597401113No Hit
CATAAAGTAGAAAGCACTACT435650.5708749843407412No Hit
CTCCCACTGCTTCACTTGACTAG386550.5065344317615369No Hit
AGCAGCATTGTACAGGGCTATGA380900.49913068182116No Hit
TTCTCACTACTGCACTTGACTAG354620.46469341661176095No Hit
TATTGCACTTGTCCCGGCCTGTA346980.454681974214508No Hit
CCCATAAAGTAGAAAGCACTAC328030.4298499279543059No Hit
TGTAAACATCCCCGACTGGAAGCT325490.4265215164766851No Hit
CCCCCCACTGCTAAATTTGACTGGCT311220.40782213388391025No Hit
ACCCCCCACTGCTAAATTTGACTG300650.3939712247034176No Hit
CTTCTCACTACTGCACTTGACTAG283430.3714061673630123No Hit
CCCCCCACTGCTAAATTTGACTGG270030.3538468312212335No Hit
CACCCGGCTGTGTGCACATGTGC254540.333548762800625No Hit
TGATGTAGTAGGTTGTATAGTT245890.3222138182016409No Hit
ACTGGACTTGGAGTCAGAAGGC233490.3059648802793979No Hit
CAAAGAATTCTCCTTTTGGGCT199810.26183066824543444No Hit
CATTGCACTTGTCTCGGTCTGA197530.25884296030489296No Hit
CTCCCACTGCTTCACTTGACTAGCCT183340.24024840956968094No Hit
AGCTCGGTCTGAGGCCCCTCAGT177700.2328577636114994No Hit
TGAGGTAGTAGTTTGTACAGTT173480.22732788312505864No Hit
TAGCAGCACGTAAATATTGGC164330.21533773941630668No Hit
CAACGGAATCCCAAAAGCAGCT162340.2127300469594306No Hit
TGTAAACATCCTACACTCTCAGCT157160.2059421841822355No Hit
CGCGACCTCAGATCAGACGTGGCG141820.18584067549455738No Hit
TGATGTAGTAGATTGTATAGTT141550.18548686797528274No Hit
TTCAAGTAATCCAGGATAGGC135310.1773099830853798No Hit
TAGCTTATCAGACTGATGTTGACA132840.17407329948312653No Hit
AACATTCAACGCTGTCGGTGAGTT128720.1686744588186393No Hit
GGAATACCGGGTGCTGTAGGCTTT126830.1661978061837168No Hit
AACGGAATCCCAAAAGCAGCTG124070.16258110709779816No Hit
CCCATAAAGTAGAAAGCACT122690.16077275755483886No Hit
CGCGACCTCAGATCAGACGTGG120220.1575360739525856No Hit
TTCAAGTAATCCAGGATAGGCTT116080.15211102532370768No Hit
TGTCAGTTTGTCAAATACCCCA113140.14825845455827263No Hit
CACTAGATTGTGAGCTCCTGGAA108950.1427678860184179No Hit
TGTCAGTTTGTCAAATACCCCAA108910.14271547008963648No Hit
CACTAGATTGTGAGCTCCTGGA108530.1422175187662129No Hit
CAAAGAATTCTCCTTTTGGGCTT105170.13781458074857283No Hit
TCCCACTGCTTCACTTGACTAG102270.13401442591191923No Hit
CCCCCACTGCTAAATTTGACTGGCT102110.1338047621967935No Hit
TATTGCACTTGTCCCGGCCTGTAG101420.13290058742531385No Hit
TTCACAGTGGCTAAGTTCCGC100470.13165570911675492No Hit
ACCGGGTGCTGTAGGCTT100270.13139362947284777No Hit
GGAATACCGGGTGCTGTAGGCTT99920.13093499009601026No Hit
TGAGGTAGTAGGTTGTGTGGTT96430.12636170030983054No Hit
CTACGCCTGTCTGAGCGTCGCT94650.12402919147905696No Hit
CAACACCAGTCGATGGGCTGT90490.11857793488578833No Hit
TCGTTTCCCGGCCAATGCACCA90120.11809308754456009No Hit
TATTGCACTTGTCCCGGCCTGTAAT89510.11729374463064329No Hit
TGTAAACATCCCCGACTGGAAGC89340.11707097693332222No Hit
TGAGGTAGTAGTTTGTGCTGTT89340.11707097693332222No Hit
TTCACAGTGGCTAAGTTCTGC87090.11412258093936682No Hit
CCCCCCACTGCTAAATTTGACTGT86440.1132708220966686No Hit
ATCACATTGCCAGGGATTTCC85960.11264183095129146No Hit
ACCGGGTGCTGTAGGCTTT85530.11207835971689109No Hit
ACCGGGTGCTGTAGGCTTA84860.11120039290980215No Hit
GGGAATACCGGGTGCTGTAGGCTT83660.10962791504635927No Hit
GACCCGCGGGCGCTCTCCAGTCCT83340.10920858761610784No Hit
TATTGCACTTGTCCCGGCCTGTT83020.10878926018585641No Hit
CAAAGTGCTGTTCGTGCAGGTAG81450.1067319349811853No Hit
TATTGCACTTGTCCCGGCCTGTAAA80190.10508083322457028No Hit
ACTTGACTTGGAGTCAGAAGGC79410.1040587226133324No Hit
GTGCGGTAACGCGACCGATCCCGGA79250.10384905889820668No Hit
TCGTTTCCCGGGCGGCGCACCA79160.10373112305844849No Hit
AGCAGCATTGTACAGGGCTATGT78420.10276142837599203No Hit
ATGCACCTGGGCAAGGATTCTGA77280.1012675744057213No Hit
TGAGAACTGAATTCCATAGGCTGT77240.10121515847693986No Hit
GGGAATACCGGGTGCTGTAGGCTTT76420.10014063193692058No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)