FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8503224
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7509568.831426762366839No Hit
CCCCCCACTGCTAAATTTGACTG6959148.184119341087568No Hit
TTCAAGTAATCCAGGATAGGCT2718323.1968109978050676No Hit
TAGCAGCACGTAAATATTGGCG2445442.8758974243181177No Hit
GCCTGTCTGAGCGTCGCT2260112.657944798349426No Hit
CCCCCACTGCTAAATTTGACTG2197562.5843844640573974No Hit
AAGCTGCCAGTTGAAGAACTGT2088092.4556450588623795No Hit
AACATTCAACGCTGTCGGTGAGT1895772.229472021435634No Hit
AACATTCAACGCTGTCGGTGAG1454141.7101043086716288No Hit
TGAGGTAGTAGGTTGTATAGTT1042551.2260643727602614No Hit
TAGCTTATCAGACTGATGTTGAC1032771.2145628528661598No Hit
TATTGCACTTGTCCCGGCCTGTAA1019411.1988511651580624No Hit
TAGCTTATCAGACTGATGTTGACT869391.0224239653100988No Hit
TATTGCACTTGTCCCGGCCTGTAT809630.9521447394541176No Hit
TAGCTTATCAGACTGATGTTGA718920.8454675544240632No Hit
TATTGCACTTGTCCCGGCCTGT671320.7894887868413204No Hit
TCGTACGACTCTTAGCGG658630.7745650355676859No Hit
TTCAAGTAATTCAGGATAGGTT590900.6949128942151824No Hit
TGTAAACATCCTTGACTGGAAGCT565730.6653123568190136No Hit
CATAAAGTAGAAAGCACTACT548180.6446731263341998No Hit
TGAGGTAGTAGATTGTATAGTT536970.6314898913635582No Hit
CAACGGAATCCCAAAAGCAGCTGT503610.5922577130744763No Hit
CCCCCACTGCTAAATTTGACTGGCTT460270.5412888099854831No Hit
TATTGCACTTGTCCCGGCCTGTA434030.5104299263432317No Hit
AGCAGCATTGTACAGGGCTATGA392510.4616013878971082No Hit
CCCATAAAGTAGAAAGCACTAC387000.455121492742047No Hit
ACTGGACTTGGAGTCAGAAGGC354710.4171476606990478No Hit
TGTAAACATCCCCGACTGGAAGCT338830.39847239117774624No Hit
TTAATGCTAATCGTGATAGGGGTT321850.3785034946744905No Hit
CACCCGGCTGTGTGCACATGTGC321740.3783741319762951No Hit
TCCTGTCTGAGCGTCGCT318130.3741286834264274No Hit
TTCTCACTACTGCACTTGACTAG289310.340235656499229No Hit
CTCCCACTGCTTCACTTGACTAG288070.338777386083208No Hit
AACCGGGCGGAAACACCA267430.3145042398036321No Hit
CATTGCACTTGTCTCGGTCTGA241710.28425688891648626No Hit
ACCCCCCACTGCTAAATTTGACTG236950.278659012158212No Hit
CCCCCCACTGCTAAATTTGACTGGCT223390.26271211954430457No Hit
TGAGAACTGAATTCCATGGGTT215290.25318632085900594No Hit
ACCGGGTGCTGTAGGCTT214970.2528099930097102No Hit
CAACGGAATCCCAAAAGCAGCT204860.2409203850210226No Hit
CCCCCCACTGCTAAATTTGACTGG203390.23919162896332027No Hit
CGCGACCTCAGATCAGACGTGGCG200930.23629860862185917No Hit
CTTCTCACTACTGCACTTGACTAG200750.23608692420663036No Hit
TAGCAGCACGTAAATATTGGC190880.22447956210491457No Hit
TGAGGTAGTAGTTTGTACAGTT187450.22044579797027572No Hit
TGTAAACATCCTACACTCTCAGCT180700.21250763239919354No Hit
CTCCCACTGCTTCACTTGACTAGCCT180130.2118372984176355No Hit
CAAAGAATTCTCCTTTTGGGCT177260.2084621080192642No Hit
ACCGGGTGCTGTAGGCTTT177220.20841506703810228No Hit
AACGGAATCCCAAAAGCAGCTG174410.20511043811147395No Hit
CCCATAAAGTAGAAAGCACT173890.20449890535636836No Hit
CGCGACCTCAGATCAGACGTGG170820.20088851005218727No Hit
ACCGGGTGCTGTAGGCTTA159560.18764647385509306No Hit
TAGCTTATCAGACTGATGTTGACA156180.18367151094690673No Hit
AGCTCGGTCTGAGGCCCCTCAGT150480.17696817113132618No Hit
CGCCTGTCTGAGCGTCGCT146580.17238167546803426No Hit
CAACACCAGTCGATGGGCTGT141100.16593706104884454No Hit
AACATTCAACGCTGTCGGTGAGTT134660.15836346308176757No Hit
TTCAAGTAATCCAGGATAGGC132190.155458682495016No Hit
TGAGGTAGTAGTTTGTGCTGTT131830.15503531366455828No Hit
TTCAAGTAATCCAGGATAGGCTT127940.15046057824655681No Hit
TTCACAGTGGCTAAGTTCCGC120870.14214608482617888No Hit
CACTAGATTGTGAGCTCCTGGA120590.14181679795804508No Hit
CTACGCCTGTCTGAGCGTCGCT120290.14146399059933032No Hit
TGATGTAGTAGGTTGTATAGTT117460.13813584118212105No Hit
TGAGAACTGAATTCCATAGGCTGT117010.1376066301440489No Hit
TATTGCACTTGTCCCGGCCTGTT115290.13558386795408425No Hit
CAAAGAATTCTCCTTTTGGGCTT113510.13349054429237664No Hit
TGAGGTAGTAGGTTGTGTGGTT111580.13122081695131166No Hit
GTGCGGTAACGCGACCGATCCCGGA110960.1304916817433011No Hit
CACTAGATTGTGAGCTCCTGGAA104070.12238887273815202No Hit
TATTGCACTTGTCCCGGCCTGTAG101530.119401770434367No Hit
CCCCCACTGCTAAATTTGACTGGCT101390.11923712700030013No Hit
CGCGACCTCAGATCAGACGTGGC99850.11742604922556432No Hit
GGAATACCGGGTGCTGTAGGCTTT97240.11435662520474588No Hit
TCGTTTCCCGGCCAATGCACCA96930.1139920576007406No Hit
TTCACAGTGGCTAAGTTCTGC94870.11156944707089922No Hit
TATTGCACTTGTCCCGGCCTGTAAT92810.10914683654105785No Hit
GGGAATACCGGGTGCTGTAGGCTT92330.10858234476711422No Hit
TGTCAGTTTGTCAAATACCCCA92200.10842946157833783No Hit
TGTCAGTTTGTCAAATACCCCAA91680.10781792882323223No Hit
ATCACATTGCCAGGGATTTCC90190.1060656522749489No Hit
TCCCACTGCTTCACTTGACTAG89680.1054658797651338No Hit
GGAATACCGGGTGCTGTAGGCTT87720.10316087168819733No Hit
CCCATAAAGTAGAAAGCACTA87010.10232589427257238No Hit
TGTAAACATCCCCGACTGGAAGC85830.1009381853282943No Hit
TTCACAGTGGCTAAGTTCCG85490.10053833698841758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)