FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947934

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947934
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7700503
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6145037.980037148222655No Hit
CAACGGAATCCCAAAAGCAGCTG5279256.855720983421472No Hit
GCCTGTCTGAGCGTCGCT4446345.774090341890653No Hit
TTCAAGTAATCCAGGATAGGCT2243132.9129655556266907No Hit
CCCCCACTGCTAAATTTGACTG2178222.828672360753577No Hit
AAGCTGCCAGTTGAAGAACTGT2158502.803063644024293No Hit
AACATTCAACGCTGTCGGTGAGT1908612.478552375085108No Hit
TAGCAGCACGTAAATATTGGCG1786592.3200951937814973No Hit
AACATTCAACGCTGTCGGTGAG1389511.8044405670642556No Hit
TATTGCACTTGTCCCGGCCTGTAA950191.23393238078084No Hit
TGAGGTAGTAGGTTGTATAGTT933761.2125961122279934No Hit
TATTGCACTTGTCCCGGCCTGT833531.0824357837403609No Hit
TATTGCACTTGTCCCGGCCTGTAT818151.0624630624778668No Hit
TAGCTTATCAGACTGATGTTGAC759050.9857148292780356No Hit
TAGCTTATCAGACTGATGTTGACT665150.8637747430265269No Hit
TAGCTTATCAGACTGATGTTGA605670.7865330355692349No Hit
ACCGGGTGCTGTAGGCTT601760.7814554451832563No Hit
TGAGGTAGTAGATTGTATAGTT521980.6778518234458191No Hit
TATTGCACTTGTCCCGGCCTGTA467210.6067265995481075No Hit
TGTAAACATCCTTGACTGGAAGCT463850.6023632482189799No Hit
ACCGGGTGCTGTAGGCTTT436910.5673785205979401No Hit
TTCAAGTAATTCAGGATAGGTT430700.5593141123378563No Hit
CATAAAGTAGAAAGCACTACT412270.5353806108510054No Hit
ACCGGGTGCTGTAGGCTTA409300.5315237199440088No Hit
TCCTGTCTGAGCGTCGCT388660.5047202760650831No Hit
TCGTACGACTCTTAGCGG350990.4558013937531094No Hit
AGCAGCATTGTACAGGGCTATGA344780.4477369854930256No Hit
CAACGGAATCCCAAAAGCAGCTGT342360.44459433364288026No Hit
CAACGGAATCCCAAAAGCAGCT339710.4411530000053243No Hit
TGAGAACTGAATTCCATGGGTT313050.4065318849950451No Hit
CGCCTGTCTGAGCGTCGCT307200.3989349786630822No Hit
ACTGGACTTGGAGTCAGAAGGC306440.3979480301481604No Hit
TGTAAACATCCCCGACTGGAAGCT290930.37780648874495604No Hit
CACCCGGCTGTGTGCACATGTGC289060.37537807595166184No Hit
AACCGGGCGGAAACACCA285570.3708459044818241No Hit
TTAATGCTAATCGTGATAGGGGTT285070.370196596248323No Hit
TTCTCACTACTGCACTTGACTAG246980.32073229502020845No Hit
CCCATAAAGTAGAAAGCACTAC244820.3179272834514836No Hit
CATTGCACTTGTCTCGGTCTGA241820.31403143405047695No Hit
CTCCCACTGCTTCACTTGACTAG222660.2891499425427144No Hit
CCCCCACTGCTAAATTTGACTGGCTT221680.2878772984050522No Hit
TGAGGTAGTAGTTTGTACAGTT206680.26839805140001893No Hit
CCCATAAAGTAGAAAGCACT181390.23555604094953278No Hit
TGAGAACTGAATTCCATAGGCTGT179410.2329847803448684No Hit
CAACACCAGTCGATGGGCTGT169770.22046611760296697No Hit
AGCTCGGTCTGAGGCCCCTCAGT162680.21125892685192124No Hit
AACGGAATCCCAAAAGCAGCTG151730.1970390765382469No Hit
TCGTTTCCCGGCCAATGCACCA147570.19163683203551768No Hit
ACCCCCCACTGCTAAATTTGACTG145610.18909154376019333No Hit
CTTCTCACTACTGCACTTGACTAG137030.17794941447331428No Hit
CGCGACCTCAGATCAGACGTGG134530.17470287330580872No Hit
CCCCCCACTGCTAAATTTGACTGG133020.17274196244063536No Hit
TGTAAACATCCTACACTCTCAGCT128280.16658652038704483No Hit
TATTGCACTTGTCCCGGCCTGTT125110.1624699061866478No Hit
TGAGGTAGTAGTTTGTGCTGTT124710.1619504595998469No Hit
CTACGCCTGTCTGAGCGTCGCT121910.1583143334922407No Hit
ATCCCGGACGAGCCCCCA121790.15815849951620042No Hit
AACATTCAACGCTGTCGGTGAGTT117680.1528211858368213No Hit
CCCCCCACTGCTAAATTTGACTGGCT117660.15279521350748126No Hit
CGCGACCTCAGATCAGACGTGGCG114410.14857470998972402No Hit
CACCCGTAGAACCGACCTTGCG114100.14817213888495337No Hit
GGAATACCGGGTGCTGTAGGCTTT112410.1459774770557196No Hit
TAGCAGCACGTAAATATTGGC112070.14553594745693885No Hit
TGATGTAGTAGGTTGTATAGTT110620.14365295357978564No Hit
CAACGGAATCCCAAAAGCAGCTGA109080.14165308422060222No Hit
CACTAGATTGTGAGCTCCTGGA108930.14145829175055188No Hit
TGAGGTAGTAGGTTGTGTGGTT108580.1410037759871011No Hit
GGAATACCGGGTGCTGTAGGCTT107270.1393025884153282No Hit
TATTGCACTTGTCCCGGCCTGTAG106180.13788709646629577No Hit
GGGAATACCGGGTGCTGTAGGCTT104370.13553660066102174No Hit
TTCAAGTAATCCAGGATAGGCTT102820.1335237451371683No Hit
TTCAAGTAATCCAGGATAGGC100350.13031616246367284No Hit
CAAAGAATTCTCCTTTTGGGCT97260.12630343758063597No Hit
CTCCCACTGCTTCACTTGACTAGCCT96780.1256801016764749No Hit
TAGCTTATCAGACTGATGTTGACA96000.12466718083221316No Hit
TTCACAGTGGCTAAGTTCTGC92300.11986229990430496No Hit
GGGAATACCGGGTGCTGTAGGCTTT90890.11803125068583183No Hit
TACGCCTGTCTGAGCGTCGCT89560.11630409078471887No Hit
AATGCACCCGGGCAAGGATTCT86640.11251213070107238No Hit
CACTAGATTGTGAGCTCCTGGAA85460.11097976327000976No Hit
TGAGGTAGTAGGTTGTATAGT84010.10909676939285654No Hit
TTCACAGTGGCTAAGTTCCG80180.10412306832423804No Hit
GTGCGGTAACGCGACCGATCCCGGA80140.10407112366555796No Hit
TTCACAGTGGCTAAGTTCCGC79540.10329195378535662No Hit
TGTAAACATCCCCGACTGGAAGC78110.10143493223754343No Hit
ACCACAGGGTAGAACCACGGAC77740.10095444414475262No Hit
GACCCGCGGGCGCTCTCCAGTCCT77320.1004090252286117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)