FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10092948
Sequences flagged as poor quality0
Sequence length18-26
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG8095928.021363034863551No Hit
CAACGGAATCCCAAAAGCAGCTG6308636.25053255005376No Hit
GCCTGTCTGAGCGTCGCT4675234.6321748611010385No Hit
CCCCCACTGCTAAATTTGACTG3145883.1169089546483346No Hit
AACATTCAACGCTGTCGGTGAGT2271982.2510568765439No Hit
TTCAAGTAATCCAGGATAGGCT2193022.1728240351580133No Hit
AAGCTGCCAGTTGAAGAACTGT2083602.06441170607438No Hit
TAGCAGCACGTAAATATTGGCG1941191.9233131885748347No Hit
AACATTCAACGCTGTCGGTGAG1758221.742028196320837No Hit
ACCGGGTGCTGTAGGCTT1122001.1116672750122165No Hit
TGAGGTAGTAGGTTGTATAGTT1072911.0630293547534377No Hit
TATTGCACTTGTCCCGGCCTGT910520.9021348371159744No Hit
TATTGCACTTGTCCCGGCCTGTAA867510.8595209249071728No Hit
TCGTACGACTCTTAGCGG845680.8378919617935215No Hit
ACCGGGTGCTGTAGGCTTT819170.8116260977466643No Hit
ACCGGGTGCTGTAGGCTTA754310.7473634066082576No Hit
TATTGCACTTGTCCCGGCCTGTAT723000.716341746732471No Hit
CGCCTGTCTGAGCGTCGCT646910.6409524749359652No Hit
TAGCTTATCAGACTGATGTTGAC641430.6355229413646042No Hit
TAGCTTATCAGACTGATGTTGA610240.604620176384541No Hit
TAGCTTATCAGACTGATGTTGACT600470.5949401502910745No Hit
TATTGCACTTGTCCCGGCCTGTA537160.5322131848890929No Hit
TGAGGTAGTAGATTGTATAGTT494230.4896785359441067No Hit
CATAAAGTAGAAAGCACTACT448490.44435976485760154No Hit
TTAATGCTAATCGTGATAGGGGTT414640.4108214963556732No Hit
TGTAAACATCCTTGACTGGAAGCT412600.40880028312837835No Hit
CAACGGAATCCCAAAAGCAGCTGT360660.3573386090961729No Hit
AGCAGCATTGTACAGGGCTATGA360310.3569918323169801No Hit
ACTGGACTTGGAGTCAGAAGGC350720.34749014856709853No Hit
TTCTCACTACTGCACTTGACTAG346630.3434378142045317No Hit
AACCGGGCGGAAACACCA335170.3320833516629631No Hit
GGAATACCGGGTGCTGTAGGCTTT323320.3203424807102939No Hit
CTACGCCTGTCTGAGCGTCGCT318730.3157947509488804No Hit
TTCAAGTAATTCAGGATAGGTT316810.31389243261730865No Hit
CCCCCACTGCTAAATTTGACTGGCTT300860.2980893193940958No Hit
TGTAAACATCCCCGACTGGAAGCT298710.29595911917905454No Hit
GGAATACCGGGTGCTGTAGGCTT295320.29260033837487326No Hit
CTCCCACTGCTTCACTTGACTAG266350.2638971289656897No Hit
CGCGACCTCAGATCAGACGTGG260980.2585765823820751No Hit
CACCCGGCTGTGTGCACATGTGC259450.25706067246160386No Hit
CCCATAAAGTAGAAAGCACTAC257020.25465305082320844No Hit
CAACACCAGTCGATGGGCTGT256960.2545936033753468No Hit
CATTGCACTTGTCTCGGTCTGA252970.25064034809254937No Hit
CCCATAAAGTAGAAAGCACT228060.22595974932200186No Hit
TGAGAACTGAATTCCATGGGTT226360.22427540496592271No Hit
CCCCCCACTGCTAAATTTGACTGG211270.20932437182872637No Hit
ATCCCGGACGAGCCCCCA209270.20734279023333918No Hit
TGAGGTAGTAGTTTGTACAGTT203030.2011602556557311No Hit
TACGCCTGTCTGAGCGTCGCT200120.19827705443444277No Hit
ACCCCCCACTGCTAAATTTGACTG198870.19703856593732577No Hit
CTTCTCACTACTGCACTTGACTAG191570.18980579311416249No Hit
CGCGACCTCAGATCAGACGTGGCG191350.1895878191386699No Hit
AGCTCGGTCTGAGGCCCCTCAGT188490.1867541574572662No Hit
GGGAATACCGGGTGCTGTAGGCTT178720.17707413136379974No Hit
ACGCCTGTCTGAGCGTCGCT175730.1741116668786959No Hit
GACCCGCGGGCGCTCTCCAGTCCT170820.16924688406202035No Hit
AACGGAATCCCAAAAGCAGCTG167610.1660664456014239No Hit
AACATTCAACGCTGTCGGTGAGTT166130.16460007522083736No Hit
TGAGGTAGTAGGTTGTGTGGTT156660.15521728636667897No Hit
CAACGGAATCCCAAAAGCAGCT154680.15325552058724567No Hit
CCCCCCACTGCTAAATTTGACTGGCT153240.15182878183856688No Hit
TGATGTAGTAGGTTGTATAGTT150770.1493815285682637No Hit
GGGAATACCGGGTGCTGTAGGCTTT148160.1467955645862834No Hit
TTCACAGTGGCTAAGTTCCG147760.146399248267206No Hit
TATTGCACTTGTCCCGGCCTGTT139310.13802706602669507No Hit
CGCGACCTCAGATCAGACGTGGC130790.12958552843034563No Hit
TAGCAGCACGTAAATATTGGC130700.1294963572585532No Hit
CTGGACGCGAGCCGGGCCCT127380.12620693181021045No Hit
CTCCCACTGCTTCACTTGACTAGCCT126670.12550347034384798No Hit
CGGCCCCGCCGGGGTCGGC126660.12549356243587106No Hit
TGAGGTAGTAGTTTGTGCTGTT124680.12353179665643775No Hit
GGGCTACGCCTGTCTGAGCGTCGC124010.12286796682198303No Hit
CCACCCTGAACGCGCCCGA123230.12209514999978203No Hit
TACCACCCTGAACGCGCCCGA122110.1209854643063652No Hit
TGTAAACATCCTACACTCTCAGCT118290.11720064345917566No Hit
ACCTGGTGCTGTAGGCTT116430.11535777257546556No Hit
ACCACCCTGAACGCGCCCGA114240.11318794072851658No Hit
CAAAGAATTCTCCTTTTGGGCT111900.11086949026191356No Hit
TTCAAGTAATCCAGGATAGGC110390.10937339615739623No Hit
TGAGGTAGTAGGTTGTATAGT104750.10378533605840433No Hit
TATTGCACTTGTCCCGGCCTGTAG103230.10227933404591007No Hit
GCGGCGTCCGGTGAGCTCTCGCT103130.10218025496614072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)