FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11835858
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG209456717.69679054953177No Hit
CAACGGAATCCCAAAAGCAGCTG11026529.316198284906765No Hit
CCCCCACTGCTAAATTTGACTG8312287.022963607708034No Hit
TTCAAGTAATCCAGGATAGGCT3786653.1993033373668394No Hit
AACATTCAACGCTGTCGGTGAGT3464972.927519069593434No Hit
AAGCTGCCAGTTGAAGAACTGT3366502.8443227352001013No Hit
TAGCAGCACGTAAATATTGGCG3268512.761531948085217No Hit
AACATTCAACGCTGTCGGTGAG2433252.055828990175448No Hit
TAGCTTATCAGACTGATGTTGACT1759021.4861786952834344No Hit
TAGCTTATCAGACTGATGTTGAC1662701.4047988747414848No Hit
TATTGCACTTGTCCCGGCCTGTAA1534651.2966106893137785No Hit
TGAGGTAGTAGGTTGTATAGTT1468561.2407718984124345No Hit
TATTGCACTTGTCCCGGCCTGTAT1247941.0543722305556555No Hit
TAGCTTATCAGACTGATGTTGA1093570.92394653602637No Hit
TGTAAACATCCTTGACTGGAAGCT987890.8346585435546793No Hit
TGAGGTAGTAGATTGTATAGTT839390.7091923542847507No Hit
TATTGCACTTGTCCCGGCCTGT712710.6021616683809488No Hit
CATAAAGTAGAAAGCACTACT682840.5769247991991794No Hit
AGCAGCATTGTACAGGGCTATGA677970.5728101841032565No Hit
GGAATACCGGGTGCTGTAGGCTTT657090.5551688774907573No Hit
CAACGGAATCCCAAAAGCAGCTGT648770.5481393913309875No Hit
TGTAAACATCCCCGACTGGAAGCT622550.5259863712457516No Hit
ACTGGACTTGGAGTCAGAAGGC566390.4785373396673059No Hit
CCCATAAAGTAGAAAGCACTAC555100.46899852972213757No Hit
TTCTCACTACTGCACTTGACTAG553550.4676889499688151No Hit
TATTGCACTTGTCCCGGCCTGTA536480.453266674878999No Hit
CCCCCACTGCTAAATTTGACTGGCTT523550.44234224506579917No Hit
CTTCTCACTACTGCACTTGACTAG477720.40362092887562523No Hit
TTCAAGTAATTCAGGATAGGTT477120.40311399477756493No Hit
CTCCCACTGCTTCACTTGACTAG448020.3785276910216395No Hit
ACCCCCCACTGCTAAATTTGACTG438660.37061951909189855No Hit
CACCCGGCTGTGTGCACATGTGC399240.33731394884933563No Hit
CCCATAAAGTAGAAAGCACT394710.33348659640898026No Hit
GGAATACCGGGTGCTGTAGGCTT380520.3214976049898537No Hit
CCCCCCACTGCTAAATTTGACTGG371680.31402877594509837No Hit
CCCCCCACTGCTAAATTTGACTGGCT342980.2897804282545465No Hit
CAAAGAATTCTCCTTTTGGGCT340450.2876428561410588No Hit
TGTAAACATCCTACACTCTCAGCT339340.2867050280596472No Hit
TGAGGTAGTAGTTTGTACAGTT336060.2839337883235842No Hit
CATTGCACTTGTCTCGGTCTGA328390.2774534807700464No Hit
CAACACCAGTCGATGGGCTGT269230.22746977870129903No Hit
CTCCCACTGCTTCACTTGACTAGCCT260800.22034735462355157No Hit
TCGTACGACTCTTAGCGG258980.21880965452610196No Hit
AACGGAATCCCAAAAGCAGCTG256980.21711987419923423No Hit
AACATTCAACGCTGTCGGTGAGTT232500.19643696299837324No Hit
CAACGGAATCCCAAAAGCAGCT230830.1950259964254387No Hit
TAGCTTATCAGACTGATGTTGACA226170.1910888082638369No Hit
CAAAGAATTCTCCTTTTGGGCTT219610.18554632879171076No Hit
TAGCAGCACGTAAATATTGGC211430.17863512725482175No Hit
GCCTGTCTGAGCGTCGCT202290.17091283116103623No Hit
TGAGGTAGTAGTTTGTGCTGTT199550.16859783211322746No Hit
CACTAGATTGTGAGCTCCTGGA182470.15416710812177706No Hit
CCCCCCACTGCTAAATTTGACTGT181860.15365172512208242No Hit
CACTAGATTGTGAGCTCCTGGAA181660.15348274708939563No Hit
TTCAAGTAATCCAGGATAGGCTT180060.15213092282790147No Hit
CCCCCCACTGCTAAATTTGACTGTA169430.1431497403905995No Hit
TGTAAACATCCCCGACTGGAAGC167670.14166273370295587No Hit
ACTGGACTTGGAGTCAGAAGGCT166730.14086853694932805No Hit
TATTGCACTTGTCCCGGCCTGTAG165730.14002364678589418No Hit
TTCAAGTAATCCAGGATAGGC163010.13772554554135408No Hit
TGAATACCGGGTGCTGTAGGCTTT160650.13573160475565016No Hit
AGCTCGGTCTGAGGCCCCTCAGT150210.12691095144940062No Hit
ACTGGACTTGGAGTCAGAAGGCA149610.1264040173513403No Hit
TTCACAGTGGCTAAGTTCCGC146610.12386934686103872No Hit
TGAGGTAGTAGGTTGTGTGGTT139750.11807340033988241No Hit
TGTCAGTTTGTCAAATACCCCAA139570.11792132011046433No Hit
CGCCTGTCTGAGCGTCGCT136890.11565701447246157No Hit
CCCCCACTGCTAAATTTGACTGGCT132970.11234504503180082No Hit
CCCCCCACTGCTAAATTTGACTGA132600.1120324356713303No Hit
TATTGCACTTGTCCCGGCCTGTAAT131830.11138187024548622No Hit
TATTGCACTTGTCCCGGCCTGTT130410.11018212621341013No Hit
TGTCAGTTTGTCAAATACCCCA127860.10802765629665378No Hit
ATCACATTGCCAGGGATTTCC125690.10619424464200229No Hit
AACATTCAACGCTGTCGGTGA123680.10449601541350022No Hit
TTCACAGTGGCTAAGTTCTGC121960.10304280433239399No Hit
TAGCAGCACATCATGGTTTACA120140.10150510423494435No Hit
CCCCCACTGCTAAATTTGACTGGCTA119880.10128543279245156No Hit
AGCAGCATTGTACAGGGCTATGT119760.10118404597283949No Hit
TGTAAACATCCTACACTCAGCT118820.10038984921921165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)