FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11349168
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG177453315.635798148375283No Hit
CAACGGAATCCCAAAAGCAGCTG10985999.679995925692527No Hit
CCCCCACTGCTAAATTTGACTG6785765.979081462182954No Hit
AACATTCAACGCTGTCGGTGAGT3498423.0825343320320924No Hit
TTCAAGTAATCCAGGATAGGCT3076412.710692096548399No Hit
TAGCAGCACGTAAATATTGGCG3029572.6694203486986887No Hit
AACATTCAACGCTGTCGGTGAG2640382.3264965326092626No Hit
AAGCTGCCAGTTGAAGAACTGT2527672.2271852879435743No Hit
TATTGCACTTGTCCCGGCCTGTAA1832691.6148232187592957No Hit
TATTGCACTTGTCCCGGCCTGTAT1606771.4157601684986953No Hit
TAGCTTATCAGACTGATGTTGACT1430531.2604712521657977No Hit
TAGCTTATCAGACTGATGTTGAC1364461.2022555309781298No Hit
TAGCTTATCAGACTGATGTTGA996880.8783727582497677No Hit
TGAGGTAGTAGGTTGTATAGTT909980.8018032687506257No Hit
TATTGCACTTGTCCCGGCCTGT847490.7467419638162022No Hit
TATTGCACTTGTCCCGGCCTGTA736430.6488845702169533No Hit
TGTAAACATCCTTGACTGGAAGCT735080.6476950557080484No Hit
CAACGGAATCCCAAAAGCAGCTGT612720.5398809851083357No Hit
AGCAGCATTGTACAGGGCTATGA567260.49982518542328386No Hit
TGTAAACATCCCCGACTGGAAGCT549790.4844319865561952No Hit
CATAAAGTAGAAAGCACTACT527750.46501206079599844No Hit
TTCTCACTACTGCACTTGACTAG517940.4563682553646223No Hit
TGAGGTAGTAGATTGTATAGTT513140.45213887044407136No Hit
TCGTACGACTCTTAGCGG504260.44431450834105196No Hit
CACCCGGCTGTGTGCACATGTGC489910.4316704096723214No Hit
ACTGGACTTGGAGTCAGAAGGC474310.4179249086805306No Hit
CCCCCACTGCTAAATTTGACTGGCTT473450.41716714388226517No Hit
TTCAAGTAATTCAGGATAGGTT455950.401747511359423No Hit
CAACGGAATCCCAAAAGCAGCT451340.39768553959197717No Hit
CTCCCACTGCTTCACTTGACTAG433300.38179010126557295No Hit
CTTCTCACTACTGCACTTGACTAG432930.38146408617794714No Hit
CCCATAAAGTAGAAAGCACTAC416800.36725159060117885No Hit
ACCCCCCACTGCTAAATTTGACTG385750.33989275689636456No Hit
CCCCCCACTGCTAAATTTGACTGG309450.2726631590967725No Hit
CATTGCACTTGTCTCGGTCTGA306770.2703017525161316No Hit
CAAAGAATTCTCCTTTTGGGCT300170.264486348250374No Hit
CCCATAAAGTAGAAAGCACT297680.26229235482283814No Hit
CCCCCCACTGCTAAATTTGACTGGCT261870.23073938107181072No Hit
AACATTCAACGCTGTCGGTGAGTT243200.2142888359745842No Hit
CACTAGATTGTGAGCTCCTGGAA238780.2103942773602435No Hit
TTAATGCTAATCGTGATAGGGGTT238290.2099625276496039No Hit
CACTAGATTGTGAGCTCCTGGA235820.20778615665923705No Hit
CTCCCACTGCTTCACTTGACTAGCCT235250.2072839171999216No Hit
AACGGAATCCCAAAAGCAGCTG226330.19942431022256435No Hit
TAGCAGCACGTAAATATTGGC225010.1982612293694128No Hit
TGTAAACATCCTACACTCTCAGCT224560.19786472453311113No Hit
TATTGCACTTGTCCCGGCCTGTAG219040.1930009318744775No Hit
TAGCTTATCAGACTGATGTTGACA218060.19213743245319834No Hit
TGAGGTAGTAGTTTGTACAGTT216130.19043686726639344No Hit
TCGTTTCCCGGCCAATGCACCA196430.17307876665496538No Hit
CAACACCAGTCGATGGGCTGT190390.1677567906299387No Hit
CAAAGAATTCTCCTTTTGGGCTT187700.16538657283071323No Hit
TATTGCACTTGTCCCGGCCTGTT170770.15046917976718646No Hit
TGAGAACTGAATTCCATGGGTT164870.14527056080234252No Hit
CCCCCCACTGCTAAATTTGACTGT162670.14333209271375663No Hit
TGTAAACATCCCCGACTGGAAGC161840.14260076157124468No Hit
CGCCCCACTGCTAAATTTGACTG151930.13386884395402376No Hit
ACTGGACTTGGAGTCAGAAGGCA149790.1319832431769448No Hit
ACTGGACTTGGAGTCAGAAGGCT149180.1314457588432914No Hit
TATTGCACTTGTCCCGGCCTGTAAT148500.1308465959795467No Hit
TGAGGTAGTAGTTTGTGCTGTT146540.12911959713698837No Hit
CCCCCCACTGCTAAATTTGACTGTA146250.1288640717980384No Hit
TGTCAGTTTGTCAAATACCCCAA143300.12626476231561645No Hit
GCCTGTCTGAGCGTCGCT141230.12444084006862882No Hit
AGCTCGGTCTGAGGCCCCTCAGT140530.12382405476771513No Hit
TTCAAGTAATCCAGGATAGGCTT140360.12367426405177895No Hit
CCCCCCACTGCTAAATTTGACTGA133240.11740067641962829No Hit
ATGCACCTGGGCAAGGATTCTGA132510.11675745746296116No Hit
TATTGCACTTGTCCCGGCCTGTAAA132380.11664291162136291No Hit
TACGCCTGTCTGAGCGTCGCT131890.11621116191072334No Hit
TATTGCACTTGTCCCGGCCTGTTT131430.11580584585583718No Hit
GGAATACCGGGTGCTGTAGGCTTT130660.11512738202483212No Hit
TTCAAGTAATCCAGGATAGGC129710.1142903162593064No Hit
CCCATAAAGTAGAAAGCACTA127640.1124663940123188No Hit
GCCCCCACTGCTAAATTTGACTG127470.1123166032963826No Hit
TGTCAGTTTGTCAAATACCCCA120820.10645714293770257No Hit
TCGTTTCCCGGGCGGCGCACCA119280.10510021527569247No Hit
CCCCCACAACCGCGCTTGACTAG118920.10478301140665112No Hit
CTACGCCTGTCTGAGCGTCGCT115950.1021660794870602No Hit
CCCCCACTGCTAAATTTGACTGGCTA113600.1000954431197071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)