FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9223909
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG132084214.319763995937079No Hit
CAACGGAATCCCAAAAGCAGCTG7065877.660385634767213No Hit
CCCCCACTGCTAAATTTGACTG5240585.681517456427638No Hit
TAGCAGCACGTAAATATTGGCG3195023.4638459681247937No Hit
AACATTCAACGCTGTCGGTGAGT3091153.3512364443317906No Hit
AAGCTGCCAGTTGAAGAACTGT2636322.8581374772886416No Hit
TTCAAGTAATCCAGGATAGGCT2411912.614845831631687No Hit
AACATTCAACGCTGTCGGTGAG2083302.258586896293101No Hit
TATTGCACTTGTCCCGGCCTGTAA1602791.7376472382804296No Hit
TATTGCACTTGTCCCGGCCTGTAT1532061.6609660828180333No Hit
TAGCTTATCAGACTGATGTTGACT1278411.3859742111506086No Hit
TAGCTTATCAGACTGATGTTGAC1237921.3420774207551267No Hit
TATTGCACTTGTCCCGGCCTGT1053721.1423790065578487No Hit
TAGCTTATCAGACTGATGTTGA944241.0236874626581853No Hit
TGAGGTAGTAGGTTGTATAGTT872010.9453800986111203No Hit
TATTGCACTTGTCCCGGCCTGTA773600.8386899740663096No Hit
TGTAAACATCCTTGACTGGAAGCT746870.809710937087519No Hit
AGCAGCATTGTACAGGGCTATGA611800.6632762747334129No Hit
CATAAAGTAGAAAGCACTACT593050.6429486674250581No Hit
TGAGGTAGTAGATTGTATAGTT541600.5871697129709323No Hit
TGTAAACATCCCCGACTGGAAGCT538950.5842967444713516No Hit
ACTGGACTTGGAGTCAGAAGGC524760.5689128112603886No Hit
TCGTACGACTCTTAGCGG426000.461843238045822No Hit
CCCATAAAGTAGAAAGCACTAC414100.44894198327411944No Hit
TTCTCACTACTGCACTTGACTAG389330.4220878588459621No Hit
TTCAAGTAATTCAGGATAGGTT385630.41807654433711344No Hit
CAACGGAATCCCAAAAGCAGCTGT385040.4174369022938106No Hit
CACCCGGCTGTGTGCACATGTGC381740.41385924340754016No Hit
CATTGCACTTGTCTCGGTCTGA336070.36434661269967No Hit
CTCCCACTGCTTCACTTGACTAG317810.34455023352897346No Hit
CCCATAAAGTAGAAAGCACT301480.32684624273721696No Hit
CTTCTCACTACTGCACTTGACTAG289770.31415097438623907No Hit
ACCCCCCACTGCTAAATTTGACTG249500.2704926945831751No Hit
CCCCCCACTGCTAAATTTGACTGG249420.27040596345865947No Hit
TTAATGCTAATCGTGATAGGGGTT236240.25611701069470655No Hit
TGAGGTAGTAGTTTGTACAGTT228660.24789923664684899No Hit
TGTAAACATCCTACACTCTCAGCT226880.24596946912637582No Hit
CACTAGATTGTGAGCTCCTGGA226800.24588273800186014No Hit
AACATTCAACGCTGTCGGTGAGTT226230.24526477873968616No Hit
TATTGCACTTGTCCCGGCCTGTAG225460.24442999166622306No Hit
ATCCCGGACGAGCCCCCA224820.2437361426700979No Hit
CAACACCAGTCGATGGGCTGT220080.2385973235425458No Hit
CGCCTGTCTGAGCGTCGCT218140.23649409377304137No Hit
AGCTCGGTCTGAGGCCCCTCAGT215610.233751221960234No Hit
CACTAGATTGTGAGCTCCTGGAA212170.23002178360606115No Hit
TATTGCACTTGTCCCGGCCTGTT195380.2118190888483397No Hit
CCCCCACTGCTAAATTTGACTGGCTT194060.2103880252938315No Hit
CAAAGAATTCTCCTTTTGGGCT193230.20948818987698167No Hit
TGAGAACTGAATTCCATGGGTT188190.2040241290324959No Hit
AACGGAATCCCAAAAGCAGCTG182280.19761686720890242No Hit
TGTCAGTTTGTCAAATACCCCAA174380.18905216866298225No Hit
GCCTGTCTGAGCGTCGCT171950.18641771075581945No Hit
TAGCAGCACGTAAATATTGGC170290.18461803992211978No Hit
TAGCTTATCAGACTGATGTTGACA164700.17855770259658893No Hit
CCCCCCACTGCTAAATTTGACTGA162360.17602081720450624No Hit
CCCCCCACTGCTAAATTTGACTGGCT158960.17233474441259122No Hit
TGTAAACATCCCCGACTGGAAGC156670.16985206597333083No Hit
TGAGGTAGTAGTTTGTGCTGTT156360.1695159828658327No Hit
CCCCCCACTGCTAAATTTGACTGT153510.16642618655496277No Hit
CAAAGAATTCTCCTTTTGGGCTT150030.1626533826385321No Hit
ACTGGACTTGGAGTCAGAAGGCA148180.16064772538410776No Hit
TTCAAGTAATCCAGGATAGGCTT141950.15389353906245173No Hit
TGAGAACTGAATTCCATAGGCTGT139880.15164937121560934No Hit
ACTGGACTTGGAGTCAGAAGGCT135160.14653223486918615No Hit
TATTGCACTTGTCCCGGCCTGTTT133140.14434227397516605No Hit
TCGTTTCCCGGGCGGCGCACCA130260.14121995349260275No Hit
ACTGGACTTGGAGTCAGAAGG122580.13289376553910062No Hit
AGCAGCATTGTACAGGGCTATGT121560.1317879437015261No Hit
TTCAAGTAATCCAGGATAGGC117480.1273646563512281No Hit
TATTGCACTTGTCCCGGCCTGTAAT115130.12481692956858095No Hit
CCCCCCACTGCTAAATTTGACTGTA113710.1232774521084282No Hit
TGTCAGTTTGTCAAATACCCCA113690.1232557693272993No Hit
CAACGGAATCCCAAAAGCAGCT113690.1232557693272993No Hit
GGAATACCGGGTGCTGTAGGCTTT113300.12283295509528552No Hit
CTCCCACTGCTTCACTTGACTAGCCT108410.11753151510926658No Hit
ACCACAGGGTAGAACCACGGAC103940.11268541352695478No Hit
CCCCCACAACCGCGCTTGACTAG103700.11242522015340786No Hit
TATTGCACTTGTCCCGGCCTGTAAA103170.11185062645349168No Hit
CAAAGTGCTGTTCGTGCAGGTAG99850.10825128478609233No Hit
AGCAGCATTGTACAGGGCTATG99630.10801277419367429No Hit
ACCGGGTGCTGTAGGCTT99440.10780678777294964No Hit
TGAGGTAGTAGGTTGTGTGGTT98860.10717798712021119No Hit
CGCGACCTCAGATCAGACGTGGCG97050.10521569542804465No Hit
CTACGCCTGTCTGAGCGTCGCT95720.10377379048297203No Hit
TATTGCACTTGTCCCGGCCTGA94590.10254871334918851No Hit
CCCATAAAGTAGAAAGCACTA94500.10245114083410842No Hit
TTCACAGTGGCTAAGTTCCG94200.10212589911717472No Hit
ACCGGGTGCTGTAGGCTTA93970.10187654713419224No Hit
TTATAAAGCAATGAGACTGATT92470.10025033854952385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)