FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5794527
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG71737312.380182196061904No Hit
CAACGGAATCCCAAAAGCAGCTG4175357.205678737884905No Hit
CCCCCACTGCTAAATTTGACTG3024875.220219010110748No Hit
AACATTCAACGCTGTCGGTGAGT1861493.2124968957776883No Hit
TTCAAGTAATCCAGGATAGGCT1851373.195032139810549No Hit
TAGCAGCACGTAAATATTGGCG1817493.1365631741814304No Hit
AAGCTGCCAGTTGAAGAACTGT1670182.8823405258099584No Hit
AACATTCAACGCTGTCGGTGAG1244242.147267585430183No Hit
TATTGCACTTGTCCCGGCCTGT764721.319728081342964No Hit
TGAGGTAGTAGGTTGTATAGTT651071.1235947299926292No Hit
TATTGCACTTGTCCCGGCCTGTAA570400.9843771545114899No Hit
TAGCTTATCAGACTGATGTTGA544410.9395244857777003No Hit
TATTGCACTTGTCCCGGCCTGTAT531410.9170895225788058No Hit
TAGCTTATCAGACTGATGTTGAC526730.9090129358272038No Hit
GCCTGTCTGAGCGTCGCT458720.7916435629689877No Hit
CATAAAGTAGAAAGCACTACT444500.7671031647621972No Hit
TAGCTTATCAGACTGATGTTGACT397500.6859921439661942No Hit
TATTGCACTTGTCCCGGCCTGTA387890.6694075288630116No Hit
TGAGGTAGTAGATTGTATAGTT361560.6239680995532509No Hit
CAACGGAATCCCAAAAGCAGCT348650.6016884553303489No Hit
CGCCTGTCTGAGCGTCGCT328500.5669142623720624No Hit
ATCCCGGACGAGCCCCCA296240.511241038310806No Hit
AGCAGCATTGTACAGGGCTATGA285650.49296517213570673No Hit
TGTAAACATCCTTGACTGGAAGCT276090.4764668453525197No Hit
CATTGCACTTGTCTCGGTCTGA260870.45020068074581415No Hit
TTCAAGTAATTCAGGATAGGTT245830.4242451540910932No Hit
TTCTCACTACTGCACTTGACTAG237880.41052531121176933No Hit
TACGCCTGTCTGAGCGTCGCT231430.3993941179323179No Hit
ACTGGACTTGGAGTCAGAAGGC225800.3896780531007967No Hit
CACCCGGCTGTGTGCACATGTGC221410.38210193860517006No Hit
CTCCCACTGCTTCACTTGACTAG217620.37556128394949234No Hit
CCCATAAAGTAGAAAGCACT217370.37512984234951363No Hit
ACCGGGTGCTGTAGGCTT213750.36888256798182145No Hit
CAACACCAGTCGATGGGCTGT211840.3655863541579839No Hit
CAACGGAATCCCAAAAGCAGCTGT201660.34801805220684967No Hit
TGTAAACATCCCCGACTGGAAGCT179580.3099131300967275No Hit
CTACGCCTGTCTGAGCGTCGCT178660.3083254250088057No Hit
TGAGGTAGTAGTTTGTACAGTT177500.30632353598490436No Hit
CCCATAAAGTAGAAAGCACTAC166410.28718478660984753No Hit
CGTTCGCGCTTTCCCCTG164090.2831810085620448No Hit
TCCTGTCTGAGCGTCGCT158150.27292995614655No Hit
CTTCTCACTACTGCACTTGACTAG141580.2443340068999592No Hit
TTCACAGTGGCTAAGTTCCG138300.23867349310823816No Hit
TTAATGCTAATCGTGATAGGGGTT136550.235653401908387No Hit
ACCGGGTGCTGTAGGCTTT123980.21396051826145604No Hit
ACCGGGTGCTGTAGGCTTA122280.21102671538160062No Hit
TGAGGTAGTAGTTTGTGCTGTT122040.210612531445621No Hit
TCGTACGACTCTTAGCGG120450.20786856286975625No Hit
AACGGAATCCCAAAAGCAGCTG120410.20779953221375966No Hit
AACATTCAACGCTGTCGGTGAGTT116460.20098275493409556No Hit
TATTGCACTTGTCCCGGCCTGTT112220.19366550539845617No Hit
TGTAAACATCCTACACTCTCAGCT105180.1815161099430549No Hit
CCCCCCACTGCTAAATTTGACTGG102160.17630429541531173No Hit
AGCTCGGTCTGAGGCCCCTCAGT100690.17376741880743674No Hit
TGAGGTAGTAGGTTGTGTGGTT98880.17064378162359067No Hit
TGAGAACTGAATTCCATGGGTT96610.16672629189578372No Hit
TATTGCACTTGTCCCGGCCTGA94450.16299863647196744No Hit
ATCCCACCGCTGCCACCA92220.15915017740015708No Hit
TTCAAGTAATCCAGGATAGGC89240.15400739352841053No Hit
CAACGGAATCCCAAAAGCAGCTGA88360.15248871909648534No Hit
TTCACAGTGGCTAAGTTCTGC86350.14901992863265628No Hit
ACCCCCCACTGCTAAATTTGACTG82210.14187525573700838No Hit
TAGCAGCACGTAAATATTGGC81520.14068447692106706No Hit
CACTAGATTGTGAGCTCCTGGA78340.13519653976933751No Hit
TTCACAGTGGCTAAGTTCCGC76810.13255611717746763No Hit
TCGTTTCCCGGCCAATGCACCA76670.13231450988147955No Hit
TCAGGCTCAGTCCCCTCCCGA75090.1295877989696139No Hit
ACGCCTGTCTGAGCGTCGCT74610.12875943109765475No Hit
AATGACACGATCACTCCCGTTG74430.12844879314567004No Hit
TCCCACTGCTTCACTTGACTAG70740.12208071512998388No Hit
TGAGGTAGTAGGTTGTATAGT70500.12166653119400428No Hit
CAAAGTGCTGTTCGTGCAGGT70190.12113154361003065No Hit
TATTGCACTTGTCCCGGCCTGTAG68760.11866369765815225No Hit
ACTGGACTTGGAGTCAGAAGG68040.11742114585021347No Hit
AGCAGCATTGTACAGGGCTATG67640.1167308392902475No Hit
ACCACAGGGTAGAACCACGGAC67260.11607504805827983No Hit
TACCACCCTGAACGCGCCCGA67120.11583344076229173No Hit
TTCACAGTGGCTAAGTTCTG64980.11214030066647374No Hit
CCCCCACTGCTAAATTTGACTGGCTT64160.11072517221854346No Hit
TCGAGGAGCTCACAGTCTAGT62420.10772233868269145No Hit
TCAGGCTCAGTCCCCTCCCGAT61940.10689397081073226No Hit
CCCCCCACTGCTAAATTTGACTGA61730.10653155986675013No Hit
CAAAGAATTCTCCTTTTGGGCT61690.10646252921075353No Hit
AATGCACCCGGGCAAGGATTCT60960.1052027197388156No Hit
AACCGGGCGGAAACACCA60170.1038393642828828No Hit
ACTGGACTTGGAGTCAGAAGGCA59900.10337340735490576No Hit
TTCAAGTAATCCAGGATAGGCTT59850.10328711903491No Hit
TGTAAACATCCCCGACTGGAAGC59830.10325260370691171No Hit
AGAGGTAGTAGGTTGCATAGTT58910.10166489861898995No Hit
AGCAGCATTGTACAGGGCTATGT58460.10088830373902821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)