FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4757388
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4369189.183989197433549No Hit
CAACGGAATCCCAAAAGCAGCTG3317926.974247212966443No Hit
GCCTGTCTGAGCGTCGCT2784765.853548207545821No Hit
CCCCCACTGCTAAATTTGACTG1905414.00515997433886No Hit
TTCAAGTAATCCAGGATAGGCT1378212.8969888518657716No Hit
TAGCAGCACGTAAATATTGGCG1260522.64960520352765No Hit
AACATTCAACGCTGTCGGTGAGT1198922.5201223864860296No Hit
AAGCTGCCAGTTGAAGAACTGT1146442.4098097527466753No Hit
TCCTGTCTGAGCGTCGCT1040632.1873977905522946No Hit
AACATTCAACGCTGTCGGTGAG852981.7929586571454756No Hit
TATTGCACTTGTCCCGGCCTGT675661.4202331195185258No Hit
TATTGCACTTGTCCCGGCCTGTAT622381.3082388907526565No Hit
TGAGGTAGTAGGTTGTATAGTT568751.1955089641626875No Hit
TATTGCACTTGTCCCGGCCTGTAA518431.0897366369949224No Hit
TAGCTTATCAGACTGATGTTGACT423390.889963147844994No Hit
TAGCTTATCAGACTGATGTTGAC412950.868018332748979No Hit
TAGCTTATCAGACTGATGTTGA403280.8476920528659844No Hit
TTCAAGTAATTCAGGATAGGTT401740.844454982439944No Hit
ATCCCGGACGAGCCCCCA349730.7351302857786668No Hit
CATAAAGTAGAAAGCACTACT346580.7285090053617658No Hit
TATTGCACTTGTCCCGGCCTGTA342490.7199118507887101No Hit
TGAGGTAGTAGATTGTATAGTT324970.6830849197080414No Hit
TCGTACGACTCTTAGCGG322080.6770101576747577No Hit
TGTAAACATCCTTGACTGGAAGCT207040.43519679286196544No Hit
AGCAGCATTGTACAGGGCTATGA198680.41762412483488837No Hit
CACCCGGCTGTGTGCACATGTGC187460.3940397545880219No Hit
CATTGCACTTGTCTCGGTCTGA185970.3909077838511385No Hit
CAACGGAATCCCAAAAGCAGCTGT175230.36833237062018065No Hit
CAACACCAGTCGATGGGCTGT173530.3647589811888372No Hit
CGCCTGTCTGAGCGTCGCT164150.3450422795029542No Hit
AACCGGGCGGAAACACCA161530.339535055791119No Hit
TTCTCACTACTGCACTTGACTAG149770.3148156089013551No Hit
ACTGGACTTGGAGTCAGAAGGC139180.29255549473786874No Hit
TGTAAACATCCCCGACTGGAAGCT138560.29125225859232No Hit
CCCATAAAGTAGAAAGCACT134640.2830124429623987No Hit
TGAGGTAGTAGTTTGTACAGTT120110.25247047329332817No Hit
TATTGCACTTGTCCCGGCCTGTT117350.24666897045185301No Hit
CCCATAAAGTAGAAAGCACTAC115540.24286436170436382No Hit
CTCCCACTGCTTCACTTGACTAG107750.2264898301336784No Hit
CAACGGAATCCCAAAAGCAGCT98400.20683618826128958No Hit
TGATGTAGTAGGTTGTATAGTT96550.2029474997624747No Hit
CCCTGTCTGAGCGTCGCT95920.20162324367909448No Hit
CACTAGATTGTGAGCTCCTGGA92810.19508604301351917No Hit
AGCTCGGTCTGAGGCCCCTCAGT90490.190209417436627No Hit
CCCCCCACTGCTAAATTTGACTGG88590.18621562924865492No Hit
AACGGAATCCCAAAAGCAGCTG85250.1791949700129567No Hit
TGAGGTAGTAGTTTGTGCTGTT81820.17198513133677557No Hit
TAGCAGCACGTAAATATTGGC76870.16158026211021678No Hit
CTTCTCACTACTGCACTTGACTAG76310.16040314559165658No Hit
TACCACCCTGAACGCGCCCGA73460.15441246330969852No Hit
TATTGCACTTGTCCCGGCCTGTAG73380.15424430380704704No Hit
AACATTCAACGCTGTCGGTGAGTT70870.14896829941135767No Hit
TGTAAACATCCTACACTCTCAGCT69520.14613060780411435No Hit
CACTAGATTGTGAGCTCCTGGAA68870.1447643118450713No Hit
CCCCCCACTGCTAAATTTGACTGT67530.14194764017565942No Hit
TTATAAAGCAATGAGACTGATT66620.14003482583299912No Hit
ACCGGGTGCTGTAGGCTT66600.13999278595733627No Hit
TTCAAGTAATCCAGGATAGGCTT63960.13444352236983825No Hit
CCCCCCACTGCTAAATTTGACTGA63880.13427536286718678No Hit
TATTGCACTTGTCCCGGCCTGA62780.13196316970572927No Hit
TATTGCACTTGTCCCGGCCTGTTT62630.13164787063825778No Hit
TTCAAGTAATCCAGGATAGGC62610.13160583076259494No Hit
ATCCCACCGCTGCCACCA60600.12738082325847713No Hit
TTCACCACCTTCTCCACCCAGC57930.12176849985748482No Hit
CAAAGTGCTGTTCGTGCAGGT57510.12088566246856469No Hit
CCCCCACTGCTAAATTTGACTGGCTT55980.11766961198035561No Hit
CGCCCCACTGCTAAATTTGACTG55580.11682881446709832No Hit
TGAGGTAGTAGGTTGTGTGGTT55310.11626127614564967No Hit
ACCCCCCACTGCTAAATTTGACTG54060.11363378391672069No Hit
TGATGTAGTAGATTGTATAGTT52910.11121649106610601No Hit
TCCGGTCTGAGCGTCGCT51460.1081686000805484No Hit
TGAGGTAGTAGGTTGTATAGT51070.10734882250512255No Hit
TCGTTTCCCGGGCGGCGCACCA50850.10688638387283106No Hit
AATGACACGATCACTCCCGTTG49970.10503662934366505No Hit
TAGCTTATCAGACTGATGTTGACA48270.10146323991232163No Hit
ACCACAGGGTAGAACCACGGAC48090.10108488103135586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)