FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7601542
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG108448114.26659222563001No Hit
CAACGGAATCCCAAAAGCAGCTG7182739.449043365148809No Hit
CCCCCACTGCTAAATTTGACTG3977555.232556762825227No Hit
AACATTCAACGCTGTCGGTGAGT2416843.179407546521482No Hit
TTCAAGTAATCCAGGATAGGCT2338613.076494216568165No Hit
TAGCAGCACGTAAATATTGGCG2178422.8657606575087002No Hit
AAGCTGCCAGTTGAAGAACTGT1796802.3637309377492093No Hit
AACATTCAACGCTGTCGGTGAG1638832.155917838775343No Hit
TATTGCACTTGTCCCGGCCTGTAA1091691.4361428247058294No Hit
TATTGCACTTGTCCCGGCCTGTAT943561.241274467732994No Hit
TAGCTTATCAGACTGATGTTGACT852301.1212198788087995No Hit
TAGCTTATCAGACTGATGTTGAC843741.1099590056859516No Hit
TGAGGTAGTAGGTTGTATAGTT661820.8706391413742107No Hit
TATTGCACTTGTCCCGGCCTGT635730.8363171577556238No Hit
TAGCTTATCAGACTGATGTTGA607110.7986669020575037No Hit
TGTAAACATCCTTGACTGGAAGCT544400.7161704822521536No Hit
CATAAAGTAGAAAGCACTACT426450.5610045961727239No Hit
TTCAAGTAATTCAGGATAGGTT423490.557110649391926No Hit
TATTGCACTTGTCCCGGCCTGTA419770.552216905464707No Hit
TGTAAACATCCCCGACTGGAAGCT395020.5196577220779678No Hit
CAACGGAATCCCAAAAGCAGCTGT384040.5052132843573054No Hit
TTCTCACTACTGCACTTGACTAG368890.48528311755693776No Hit
AGCAGCATTGTACAGGGCTATGA360350.4740485548853114No Hit
TGAGGTAGTAGATTGTATAGTT359300.472667256196177No Hit
CACCCGGCTGTGTGCACATGTGC316600.4164944428380452No Hit
ACTGGACTTGGAGTCAGAAGGC280600.3691356306391519No Hit
CCCCCACTGCTAAATTTGACTGGCTT261430.3439170631432412No Hit
CCCATAAAGTAGAAAGCACTAC258790.340444083581989No Hit
TTAATGCTAATCGTGATAGGGGTT249010.327578272934623No Hit
TCGTACGACTCTTAGCGG232230.3055038043596944No Hit
CATTGCACTTGTCTCGGTCTGA228200.3002022484385405No Hit
CTTCTCACTACTGCACTTGACTAG224140.2948612268405542No Hit
CAACGGAATCCCAAAAGCAGCT206360.2714712357045452No Hit
CCCCCCACTGCTAAATTTGACTGG189110.24877847152590885No Hit
CAAAGAATTCTCCTTTTGGGCT175250.2305453288293349No Hit
ACCCCCCACTGCTAAATTTGACTG172770.22728283287785558No Hit
CCCATAAAGTAGAAAGCACT170870.2247833400118029No Hit
AACATTCAACGCTGTCGGTGAGTT158200.20811566916291457No Hit
TGAGAACTGAATTCCATGGGTT155130.20407701490039784No Hit
TAGCAGCACGTAAATATTGGC153440.201853781772172No Hit
TGATGTAGTAGGTTGTATAGTT149070.1961049481802508No Hit
TGTCAGTTTGTCAAATACCCCAA147320.19380278369836015No Hit
AACGGAATCCCAAAAGCAGCTG147130.1935528344117549No Hit
TGAGGTAGTAGTTTGTACAGTT143350.1885801591308711No Hit
TCGTTTCCCGGCCAATGCACCA142710.18773822469177964No Hit
CACTAGATTGTGAGCTCCTGGA142070.1868962902526882No Hit
TGTAAACATCCTACACTCTCAGCT141670.18637008122825605No Hit
CAACACCAGTCGATGGGCTGT137050.18029236699606474No Hit
TAGCTTATCAGACTGATGTTGACA131730.17329378697111716No Hit
CACTAGATTGTGAGCTCCTGGAA131670.17321485561745234No Hit
CAAAGAATTCTCCTTTTGGGCTT131360.17280704362351745No Hit
CTCCCACTGCTTCACTTGACTAG129420.17025492985502153No Hit
CCCCCCACTGCTAAATTTGACTGGCT125100.1645718723911543No Hit
TGTAAACATCCCCGACTGGAAGC122160.16070423606157802No Hit
TATTGCACTTGTCCCGGCCTGTAG122000.16049375245180517No Hit
GGAATACCGGGTGCTGTAGGCTTT119330.15698130721372058No Hit
TATTGCACTTGTCCCGGCCTGTT115630.15211387373772323No Hit
CCCCCCACTGCTAAATTTGACTGT114100.15010112421927024No Hit
CCCCCCACTGCTAAATTTGACTGTA112080.1474437686458879No Hit
TGAGGTAGTAGTTTGTGCTGTT112060.14741745819466628No Hit
GCCCCCACTGCTAAATTTGACTG109940.1446285503651759No Hit
TTCAAGTAATCCAGGATAGGCTT105820.1392085974135248No Hit
TGTCAGTTTGTCAAATACCCCA105330.1385639913585954No Hit
GACCCGCGGGCGCTCTCCAGTCCT97730.1285660198943846No Hit
TATTGCACTTGTCCCGGCCTGTAAT94930.12488255672335954No Hit
AGCTCGGTCTGAGGCCCCTCAGT94050.12372489686960882No Hit
TTCAAGTAATCCAGGATAGGC91390.12022560685713504No Hit
TATTGCACTTGTCCCGGCCTGTAAA89650.11793659760085519No Hit
GGAATACCGGGTGCTGTAGGCTT89600.11787082147280117No Hit
CGCCTGTCTGAGCGTCGCT88730.11672631684466125No Hit
ACTGGACTTGGAGTCAGAAGGCT83770.11020132494170261No Hit
TGAGAACTGAATTCCATAGGCTGT82290.10825435155130365No Hit
TGATGTAGTAGATTGTATAGTT81990.10785969478297956No Hit
ATCCCGGACGAGCCCCCA80240.10555753030108891No Hit
CAACGGAATCCCAAAAGCAGCTGA79820.10500501082543515No Hit
CTCCCACTGCTTCACTTGACTAGCCT79030.10396574800218165No Hit
CCCCCACTGCTAAATTTGACTGGCTA76860.10111106404463725No Hit
TATTGCACTTGTCCCGGCCTGTTT76380.10047961321531869No Hit
CGCCCCACTGCTAAATTTGACTG76320.10040068186165385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)