FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6327519
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG105379916.654221030391216No Hit
CAACGGAATCCCAAAAGCAGCTG4840597.650059999819835No Hit
CCCCCACTGCTAAATTTGACTG4366126.900208438726143No Hit
AACATTCAACGCTGTCGGTGAGT2072483.2753437800818928No Hit
AACATTCAACGCTGTCGGTGAG1388362.1941617243662166No Hit
TTCAAGTAATCCAGGATAGGCT1329482.10110787498228No Hit
TAGCAGCACGTAAATATTGGCG1277082.0182950063049985No Hit
TATTGCACTTGTCCCGGCCTGTAA1195761.8897770200294934No Hit
TAGCTTATCAGACTGATGTTGAC1100251.7388331824843197No Hit
AAGCTGCCAGTTGAAGAACTGT1068281.688307850201635No Hit
TAGCTTATCAGACTGATGTTGACT1054161.6659926268099707No Hit
TATTGCACTTGTCCCGGCCTGTAT973821.5390234308265214No Hit
GCCTGTCTGAGCGTCGCT722551.141916760739873No Hit
TAGCTTATCAGACTGATGTTGA587940.9291793513381784No Hit
TGAGGTAGTAGGTTGTATAGTT524520.8289504938665534No Hit
TATTGCACTTGTCCCGGCCTGT485390.7671095100623168No Hit
GGAATACCGGGTGCTGTAGGCTTT480930.7600609338352047No Hit
TATTGCACTTGTCCCGGCCTGTA430660.6806143134457596No Hit
TGTAAACATCCTTGACTGGAAGCT382240.6040914298321348No Hit
TGTAAACATCCCCGACTGGAAGCT340580.5382520384371821No Hit
AGCAGCATTGTACAGGGCTATGA326880.5166005823135419No Hit
GGAATACCGGGTGCTGTAGGCTT316450.5001170284909456No Hit
TGAGGTAGTAGATTGTATAGTT301080.47582630727778136No Hit
CACCCGGCTGTGTGCACATGTGC298280.47140119215762133No Hit
TCCTGTCTGAGCGTCGCT289450.4574462755465452No Hit
TTCAAGTAATTCAGGATAGGTT272830.43118005651188085No Hit
CAACGGAATCCCAAAAGCAGCT263890.41705129609251274No Hit
ACTGGACTTGGAGTCAGAAGGC257590.40709478707215263No Hit
CAACGGAATCCCAAAAGCAGCTGT252380.39886091215214053No Hit
CATAAAGTAGAAAGCACTACT244090.38575941059995233No Hit
CATTGCACTTGTCTCGGTCTGA235780.3726263010826202No Hit
TGAATACCGGGTGCTGTAGGCTTT209000.3303032357548037No Hit
TTAATGCTAATCGTGATAGGGGTT204470.32314403164968764No Hit
TTCTCACTACTGCACTTGACTAG193880.3064076141059395No Hit
TGATGTAGTAGGTTGTATAGTT187810.29681459668473537No Hit
CACTAGATTGTGAGCTCCTGGA172330.2723500316632791No Hit
CCCCCACTGCTAAATTTGACTGGCTT171140.2704693577372111No Hit
TAGCTTATCAGACTGATGTTGACA166240.262725406276931No Hit
CACTAGATTGTGAGCTCCTGGAA164600.26013355313512293No Hit
CGCCCCACTGCTAAATTTGACTG155580.24587836085517878No Hit
CCCATAAAGTAGAAAGCACTAC153420.24246470061962672No Hit
CTCCCCACTGCTAAATTTGACTG149340.23601667573025067No Hit
TGTCAGTTTGTCAAATACCCCAA142450.22512773173814252No Hit
TGAATACCGGGTGCTGTAGGCTT139350.2202284971408225No Hit
AACATTCAACGCTGTCGGTGAGTT135110.21352760853029443No Hit
TATTGCACTTGTCCCGGCCTGTAG134980.2133221567568584No Hit
CAAAGAATTCTCCTTTTGGGCT134970.21330635277428645No Hit
CAACACCAGTCGATGGGCTGT131330.20755370311807833No Hit
CCCGCCACTGCTAAATTTGACTG129120.20406102296966633No Hit
CTTCTCACTACTGCACTTGACTAG126630.20012583130923828No Hit
TATTGCACTTGTCCCGGCCTGTAAT112490.17777899995243No Hit
CCCCCCACTGCTAAATTTGACTGG111890.17683076099811No Hit
ACCCCCCACTGCTAAATTTGACTG111570.176325033555806No Hit
TCGTACGACTCTTAGCGG110880.175234558758338No Hit
TATTGCACTTGTCCCGGCCTGTAAA106680.16859688607809792No Hit
CAAAGAATTCTCCTTTTGGGCTT106310.1680121387229339No Hit
TGATGTAGTAGATTGTATAGTT105980.1674906072980579No Hit
CCCATAAAGTAGAAAGCACT105720.1670797037511859No Hit
TAGCAGCACGTAAATATTGGC101960.16113740630411383No Hit
ACTTGACTTGGAGTCAGAAGGC101730.16077391470495783No Hit
TGAGGTAGTAGTTTGTACAGTT100980.1595886160120578No Hit
CCCCCCACTGCTAAATTTGACTGTA99940.1579450018245698No Hit
CCCCCCACTGCTAAATTTGACTGT98590.15581146417734976No Hit
TGTAAACATCCTACACTCTCAGCT98440.15557440443876977No Hit
CAACGGAATCCCAAAAGCAGCTGA94740.14972693088712968No Hit
TATTGCACTTGTCCCGGCCTGTT92980.14694542995445767No Hit
AACGGAATCCCAAAAGCAGCTG89880.14204619535713758No Hit
TGAGGTAGTAGTTTGTGCTGTT85770.13555075852004553No Hit
ACTGGACTTGGAGTCAGAAGGCT82280.13003516860241746No Hit
TGTCAGTTTGTCAAATACCCCA81440.12870763406636945No Hit
TGAGAACTGAATTCCATAGGCTGT79440.1255468375519694No Hit
TGTAAACATCCCCGACTGGAAGC78600.1242193030159214No Hit
TTCAAGTAATCCAGGATAGGCTT77630.1226863167064374No Hit
TTATAAAGCAATGAGACTGATT77000.12169066580440138No Hit
TGAGAACTGAATTCCATGGGTT76590.12104270251894937No Hit
ATGCACCTGGGCAAGGATTCTGA76480.12086885871065738No Hit
ACTGGACTTGGAGTCAGAAGGCA75370.11911461664516536No Hit
CACCCGTAGAACCGACCTTGCG75140.11875112504600935No Hit
GCCCCCACTGCTAAATTTGACTG74730.11810316176055734No Hit
GGAATACCGGGTGCTGTAGGCTTA73910.11680723518965332No Hit
TGCAAGTAATCCAGGATAGGCT68640.10847853637420922No Hit
CCCCCCACTGCTAAATTTGACTGGCT65680.10380055753289719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)