FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11559944
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG146132512.64128096122265No Hit
CAACGGAATCCCAAAAGCAGCTG6144555.315380420527989No Hit
CCCCCACTGCTAAATTTGACTG5404684.675351368484138No Hit
AACATTCAACGCTGTCGGTGAGT4337213.7519299401450388No Hit
TTCAAGTAATCCAGGATAGGCT3937653.4062881273473296No Hit
AAGCTGCCAGTTGAAGAACTGT3708513.2080691740375213No Hit
TAGCAGCACGTAAATATTGGCG3609003.1219874421536993No Hit
TAGCTTATCAGACTGATGTTGAC1967541.7020324665932638No Hit
AACATTCAACGCTGTCGGTGAG1914871.656469962138225No Hit
TAGCTTATCAGACTGATGTTGACT1743211.5079744330941396No Hit
TATTGCACTTGTCCCGGCCTGTAA1324921.1461301196614795No Hit
TGTAAACATCCTTGACTGGAAGCT1219701.0551089174826453No Hit
TATTGCACTTGTCCCGGCCTGTAT1157171.0010169599437506No Hit
TAGCTTATCAGACTGATGTTGA1094250.9465876305283141No Hit
TGAGGTAGTAGGTTGTATAGTT1032820.8934472346924864No Hit
TATTGCACTTGTCCCGGCCTGT976860.8450386956891832No Hit
CATAAAGTAGAAAGCACTACT850240.7355052931052262No Hit
AGCAGCATTGTACAGGGCTATGA737940.6383594937830149No Hit
TGTAAACATCCCCGACTGGAAGCT705640.6104181819565907No Hit
TATTGCACTTGTCCCGGCCTGTA678800.5872000764017542No Hit
TTCTCACTACTGCACTTGACTAG600120.5191374629496476No Hit
ACTGGACTTGGAGTCAGAAGGC591480.5116633783001024No Hit
CGCCTGTCTGAGCGTCGCT532860.46095379008756443No Hit
CCCATAAAGTAGAAAGCACTAC517270.44746756558682294No Hit
TTCAAGTAATTCAGGATAGGTT496880.4298290718363342No Hit
TGAGGTAGTAGATTGTATAGTT494180.42749342038335136No Hit
TTAATGCTAATCGTGATAGGGGTT488960.42297782757425123No Hit
CAACGGAATCCCAAAAGCAGCTGT438760.3795520116706448No Hit
AACATTCAACGCTGTCGGTGAGTT402660.3483234866881708No Hit
TGTAAACATCCTACACTCTCAGCT395520.34214698617917183No Hit
CTTCTCACTACTGCACTTGACTAG365560.3162299056119995No Hit
GGAATACCGGGTGCTGTAGGCTTT364500.3153129461526803No Hit
GGAATACCGGGTGCTGTAGGCTT359450.31094441287950875No Hit
CCCCCACTGCTAAATTTGACTGGCTT330390.2858058827966641No Hit
CAAAGAATTCTCCTTTTGGGCT318660.27565877481759427No Hit
CCCCCCACTGCTAAATTTGACTGG296760.2567140463656225No Hit
CATTGCACTTGTCTCGGTCTGA294950.25514829483603035No Hit
TAGCTTATCAGACTGATGTTGACA260760.2255720269925183No Hit
CTACGCCTGTCTGAGCGTCGCT255590.22109968698810306No Hit
CAAAGAATTCTCCTTTTGGGCTT249160.21553737630562916No Hit
TCGTACGACTCTTAGCGG240460.20801138829046228No Hit
CCCATAAAGTAGAAAGCACT230230.1991618644519385No Hit
TACGCCTGTCTGAGCGTCGCT229140.19821895330980843No Hit
AGCTCGGTCTGAGGCCCCTCAGT222300.19230196962891863No Hit
TTCAAGTAATCCAGGATAGGCTT220310.1905805080024609No Hit
CACCCGGCTGTGTGCACATGTGC220150.19044209902746934No Hit
TGAGGTAGTAGTTTGTACAGTT217100.18780367794169245No Hit
TTCACAGTGGCTAAGTTCCG215540.1864541904355246No Hit
CCCCCCACTGCTAAATTTGACTGGCT212010.183400542424773No Hit
TGTCAGTTTGTCAAATACCCCAA207180.17922232149221484No Hit
TGATGTAGTAGGTTGTATAGTT199830.1728641592035394No Hit
CAACACCAGTCGATGGGCTGT197520.17086587962709854No Hit
CACTAGATTGTGAGCTCCTGGA194100.16790738778665362No Hit
TGTAAACATCCCCGACTGGAAGC191250.1654419779196162No Hit
TATTGCACTTGTCCCGGCCTGTT185120.16013918406525152No Hit
CACTAGATTGTGAGCTCCTGGAA181480.1569903798841932No Hit
GACCCGCGGGCGCTCTCCAGTCCT176600.15276890614695018No Hit
CTCCCACTGCTTCACTTGACTAG173040.14968930645338768No Hit
TGAGGTAGTAGTTTGTGCTGTT172220.14897996045655584No Hit
TAGCAGCACGTAAATATTGGC166800.14429135642871627No Hit
TTCAAGTAATCCAGGATAGGC165960.1435647093100105No Hit
TATTGCACTTGTCCCGGCCTGTAG165240.14294186892254843No Hit
ACGCCTGTCTGAGCGTCGCT164640.14242283526633No Hit
CAACGGAATCCCAAAAGCAGCT163080.14107334776016217No Hit
ACTGGACTTGGAGTCAGAAGGCA162020.14015638830084298No Hit
TTATAAAGCAATGAGACTGATT153730.13298507328409204No Hit
GCGTTCGCGCTTTCCCCTG153100.1324400879450627No Hit
TGTCAGTTTGTCAAATACCCCA151770.13128956334044525No Hit
ACCCCCCACTGCTAAATTTGACTG150930.1305629162217395No Hit
CTCCCCACTGCTAAATTTGACTG149290.12914422422807584No Hit
CCCCCCACTGCTAAATTTGACTGTA148310.12829646925625246No Hit
CCCGCCACTGCTAAATTTGACTG144110.12466323366272362No Hit
TAGCAGCACGTAAATATTGGCGT142150.12296772371907683No Hit
TGTAAACATCCTACACTCAGCT139320.12051961497391336No Hit
CAAAGTGCTGTTCGTGCAGGTAG137860.11925663307711526No Hit
ACTGGACTTGGAGTCAGAAGG136600.11816666239905661No Hit
AGCAGCATTGTACAGGGCTATGT136370.11796769949750621No Hit
CGCCCCACTGCTAAATTTGACTG135940.11759572537721637No Hit
ACTTGACTTGGAGTCAGAAGGC135280.11702478835537611No Hit
CCCCCCACTGCTAAATTTGACTGA132010.11419605492898582No Hit
CAAAGTGCTGTTCGTGCAGGT131380.11365106958995648No Hit
ACCACAGGGTAGAACCACGGAC128430.11109915411354934No Hit
TGCAAGTAATCCAGGATAGGCT127110.10995728006986887No Hit
TGAATACCGGGTGCTGTAGGCTTT126530.1094555475355244No Hit
AACGGAATCCCAAAAGCAGCTG126060.10904897117148664No Hit
AGCAGCATTGTACAGGGCTATG124370.10758702637313813No Hit
TGAATACCGGGTGCTGTAGGCTT124160.1074053645934617No Hit
CCCCCCACTGCTAAATTTGACTGT124160.1074053645934617No Hit
CCCCCACTGCTAAATTTGACTGGCTA121760.10532922996858808No Hit
AACATTCAACGCTGTCGGTGA120790.10449012555770165No Hit
ACTGGACTTGGAGTCAGAAGGCT120200.10397974246242024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)