FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10658558
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG133195612.496587249419667No Hit
CAACGGAATCCCAAAAGCAGCTG6096165.719497890802865No Hit
CCCCCACTGCTAAATTTGACTG5234084.910683039863366No Hit
AACATTCAACGCTGTCGGTGAGT4214373.953977639376734No Hit
TAGCAGCACGTAAATATTGGCG3202943.005040644334815No Hit
TTCAAGTAATCCAGGATAGGCT3039152.8513707013650436No Hit
AAGCTGCCAGTTGAAGAACTGT2938812.7572303870748742No Hit
AACATTCAACGCTGTCGGTGAG1893731.7767225172485808No Hit
TAGCTTATCAGACTGATGTTGAC1823631.7109537706695408No Hit
TAGCTTATCAGACTGATGTTGACT1545891.450374431513156No Hit
TATTGCACTTGTCCCGGCCTGTAA1236051.159678448060235No Hit
TAGCTTATCAGACTGATGTTGA1094301.0266867244143159No Hit
TGTAAACATCCTTGACTGGAAGCT1016590.9537781752465953No Hit
TATTGCACTTGTCCCGGCCTGTAT994660.9332031593767187No Hit
TATTGCACTTGTCCCGGCCTGT837670.7859130662890796No Hit
TGAGGTAGTAGGTTGTATAGTT804360.7546611839988111No Hit
AGCAGCATTGTACAGGGCTATGA719560.6751007031157499No Hit
CGCCTGTCTGAGCGTCGCT671230.6297568582917126No Hit
CATAAAGTAGAAAGCACTACT646410.6064704062219298No Hit
TGTAAACATCCCCGACTGGAAGCT600520.5634158016497166No Hit
ACTGGACTTGGAGTCAGAAGGC575080.5395476573847982No Hit
TATTGCACTTGTCCCGGCCTGTA573230.5378119629315711No Hit
TTCAAGTAATTCAGGATAGGTT520800.48862144391389534No Hit
TTCTCACTACTGCACTTGACTAG488760.45856109241043674No Hit
TGAGGTAGTAGATTGTATAGTT474310.4450039114109057No Hit
AACATTCAACGCTGTCGGTGAGTT391270.3670946857914551No Hit
CAACGGAATCCCAAAAGCAGCTGT375590.35238350253383244No Hit
CCCATAAAGTAGAAAGCACTAC365300.3427292885210176No Hit
CCCCCACTGCTAAATTTGACTGGCTT346620.3252034656095131No Hit
CTTCTCACTACTGCACTTGACTAG332350.3118151629892149No Hit
CATTGCACTTGTCTCGGTCTGA290890.27291684297256724No Hit
TGTAAACATCCTACACTCTCAGCT278210.2610202993688264No Hit
CCCCCCACTGCTAAATTTGACTGG247770.2324610890141049No Hit
TAGCTTATCAGACTGATGTTGACA247590.23229221063487202No Hit
TACGCCTGTCTGAGCGTCGCT246990.2317292827040956No Hit
CACCCGGCTGTGTGCACATGTGC245500.23033134500933428No Hit
CAACGGAATCCCAAAAGCAGCT232880.21849109419867116No Hit
CTACGCCTGTCTGAGCGTCGCT227700.2136311497296351No Hit
CAAAGAATTCTCCTTTTGGGCT223070.20928722253047738No Hit
CCCATAAAGTAGAAAGCACT220630.20699798227865346No Hit
TGTCAGTTTGTCAAATACCCCAA219800.20621926530774615No Hit
CCCCCCACTGCTAAATTTGACTGGCT214740.20147190642486537No Hit
TCGTACGACTCTTAGCGG211680.19860097397790583No Hit
CAAAGAATTCTCCTTTTGGGCTT208450.19557054528389298No Hit
TGAGGTAGTAGTTTGTACAGTT200210.18783966836789742No Hit
TTCAAGTAATCCAGGATAGGCTT198220.18597262406415577No Hit
CTCCCACTGCTTCACTTGACTAG192590.18069048364703744No Hit
CAACACCAGTCGATGGGCTGT182730.17143970131794564No Hit
ACGCCTGTCTGAGCGTCGCT179630.16853124034226769No Hit
CGCCCCACTGCTAAATTTGACTG176170.16528502260812392No Hit
TGTAAACATCCCCGACTGGAAGC171960.16133514496050966No Hit
TGATGTAGTAGGTTGTATAGTT170960.16039693174254904No Hit
TAGCAGCACGTAAATATTGGC169220.15876444074329754No Hit
TTATAAAGCAATGAGACTGATT160180.15028299325293346No Hit
TTCACAGTGGCTAAGTTCCG157270.14755279278866804No Hit
TATTGCACTTGTCCCGGCCTGTT155490.14588277326069812No Hit
TGAGGTAGTAGTTTGTGCTGTT153640.14414707880747094No Hit
TGTCAGTTTGTCAAATACCCCA149440.1402065832920363No Hit
ACTGGACTTGGAGTCAGAAGGCA148950.1397468588152356No Hit
GCGTTCGCGCTTTCCCCTG144980.13602215233993192No Hit
ACCCCCCACTGCTAAATTTGACTG141440.13270087754835128No Hit
TCGTTTCCCGGCCAATGCACCA139620.13099332949166295No Hit
ACTGGACTTGGAGTCAGAAGG136880.1284226252744508No Hit
TATTGCACTTGTCCCGGCCTGTAG135710.1273249158094369No Hit
AACGGAATCCCAAAAGCAGCTG134690.12636793832711704No Hit
AGCAGCATTGTACAGGGCTATGT133590.12533590378736034No Hit
CTCCCCACTGCTAAATTTGACTG131540.12341256669054107No Hit
CACTAGATTGTGAGCTCCTGGA129920.12189266127744486No Hit
ACTTGACTTGGAGTCAGAAGGC127450.1195752746290821No Hit
TTCAAGTAATCCAGGATAGGC127050.11919998934189784No Hit
TAGCAGCACGTAAATATTGGCGT125750.11798031215854902No Hit
TTTAAACATCCTTGACTGGAAGCT124600.11690136695789431No Hit
CTCCCACTGCTTCACTTGACTAGCCT124170.11649793527417124No Hit
CACTAGATTGTGAGCTCCTGGAA122610.11503432265415264No Hit
AGCAGCATTGTACAGGGCTATG122480.11491235493581778No Hit
ACCACAGGGTAGAACCACGGAC120350.11291396078156163No Hit
ACTGGACTTGGAGTCAGAAGGCT119710.11231350432206683No Hit
GACCCGCGGGCGCTCTCCAGTCCT116170.1089922295304862No Hit
CAAAGTGCTGTTCGTGCAGGTAG113840.10680619273263794No Hit
TGCAAGTAATCCAGGATAGGCT113830.10679681060045834No Hit
CCCCCCACTGCTAAATTTGACTGA113250.10625264693404117No Hit
CAAAGTGCTGTTCGTGCAGGT112530.10557713341710952No Hit
CCCCCCACTGCTAAATTTGACTGTA111710.1048077985783818No Hit
CCCCCACTGCTAAATTTGACTGGCTA109520.10275311163104803No Hit
AGCTCGGTCTGAGGCCCCTCAGT109230.10248102979783945No Hit
AACATTCAACGCTGTCGGTGA106970.10036066792524843No Hit
CCCGCCACTGCTAAATTTGACTG106680.10008858609203986No Hit
TGTAAACATCCTACACTCAGCT106640.1000510575633214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)