FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10515995
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG126027111.984324830888566No Hit
CCCCCCACTGCTAAATTTGACTG8932268.493975130265847No Hit
GCCTGTCTGAGCGTCGCT5960675.6681940225342435No Hit
TAGCAGCACGTAAATATTGGCG5736055.454595594615631No Hit
TTCAAGTAATCCAGGATAGGCT4896374.656116706027342No Hit
AAGCTGCCAGTTGAAGAACTGT4008463.8117743494552823No Hit
CCCCCACTGCTAAATTTGACTG3575833.4003724802075315No Hit
AACATTCAACGCTGTCGGTGAGT1544191.4684202493439755No Hit
TGAGGTAGTAGGTTGTATAGTT1396091.3275871660266099No Hit
AACATTCAACGCTGTCGGTGAG1277081.2144167052190495No Hit
CATAAAGTAGAAAGCACTACT1269951.2076365574536694No Hit
TAGCTTATCAGACTGATGTTGA1146301.0900537704706021No Hit
TAGCTTATCAGACTGATGTTGAC1088631.0352135009573513No Hit
AACCGGGCGGAAACACCA1016650.966765389295069No Hit
TATTGCACTTGTCCCGGCCTGT930280.884633360894523No Hit
CAACGGAATCCCAAAAGCAGCTGT909840.8651963033455227No Hit
TAGCTTATCAGACTGATGTTGACT884780.8413659382683236No Hit
TATTGCACTTGTCCCGGCCTGTAA863880.8214914518312343No Hit
TATTGCACTTGTCCCGGCCTGTAT830660.7899014786522817No Hit
TGTAAACATCCTTGACTGGAAGCT659100.6267595220423745No Hit
TTCAAGTAATTCAGGATAGGTT651980.6199888836006483No Hit
TGAGGTAGTAGATTGTATAGTT646200.6144924945285729No Hit
CCCATAAAGTAGAAAGCACTAC623530.5929348578047061No Hit
TCGTACGACTCTTAGCGG573240.545112469148188No Hit
AGCAGCATTGTACAGGGCTATGA523490.49780358396899194No Hit
CATTGCACTTGTCTCGGTCTGA474900.4515977803336726No Hit
CAACGGAATCCCAAAAGCAGCT450870.4287468755928469No Hit
TATTGCACTTGTCCCGGCCTGTA436670.41524363600401104No Hit
ACTGGACTTGGAGTCAGAAGGC413120.3928491787985825No Hit
CCCATAAAGTAGAAAGCACT410450.39031018938293527No Hit
TAGCAGCACGTAAATATTGGC375130.35672325823661954No Hit
CAACACCAGTCGATGGGCTGT335610.3191424111555778No Hit
TTCTCACTACTGCACTTGACTAG328850.31271410836539953No Hit
AACGGAATCCCAAAAGCAGCTG308120.29300128043043006No Hit
TGTAAACATCCCCGACTGGAAGCT299800.2850895231502107No Hit
CACCCGGCTGTGTGCACATGTGC286830.27275593037083035No Hit
TTCAAGTAATCCAGGATAGGC284710.27073995375615906No Hit
TGTAAACATCCTACACTCTCAGCT261250.2484310804636176No Hit
TTCACAGTGGCTAAGTTCCGC256580.2439902263171483No Hit
CTTCTCACTACTGCACTTGACTAG254500.24201228699709348No Hit
CTCCCACTGCTTCACTTGACTAG250940.2386269677762304No Hit
TGAGGTAGTAGTTTGTACAGTT242580.23067717320139464No Hit
TTCACAGTGGCTAAGTTCCG230450.2191423636089595No Hit
CCCATAAAGTAGAAAGCACTA216040.20543942822338732No Hit
CACTAGATTGTGAGCTCCTGGA207330.1971568073206577No Hit
TGATGTAGTAGGTTGTATAGTT201760.19186011404531858No Hit
ACCGGGTGCTGTAGGCTT191990.18256950483525333No Hit
TGAGGTAGTAGTTTGTGCTGTT191900.18248392092236634No Hit
CAAAGAATTCTCCTTTTGGGCT190680.18132378343656497No Hit
TTCACAGTGGCTAAGTTCTGC182410.17345957277461618No Hit
ACCCCCCACTGCTAAATTTGACTG177900.16917086780661267No Hit
CCCCCACTGCTAAATTTGACTGGCTT168630.16035572477925294No Hit
TTCAAGTAATCCAGGATAGGCTT157460.14973381025761234No Hit
CCCCCCACTGCTAAATTTGACTGG157050.14934392798779383No Hit
GGAATACCGGGTGCTGTAGGCTTT156890.14919177880932807No Hit
GGAATACCGGGTGCTGTAGGCTT152830.14533099340575953No Hit
AGCTCGGTCTGAGGCCCCTCAGT152200.14473190601555058No Hit
TAGCAGCACGTAAATATTGGCGT150920.14351471258782456No Hit
TATTGCACTTGTCCCGGCCTGTT144970.13785666501362923No Hit
ACCGGGTGCTGTAGGCTTT144670.13757138530400592No Hit
ATCACATTGCCAGGGATTTCC135870.12920318048838936No Hit
TCAGTGCACTACAGAACTTTGT135690.12903201266261538No Hit
AATGACACGATCACTCCCGTTG135230.12859458377452632No Hit
TAGCTTATCAGACTGATGTTGACA133560.12700652672429No Hit
CCCCCCACTGCTAAATTTGACTGGCT130410.12401108977324542No Hit
TCGTTTCCCGGCCAATGCACCA130410.12401108977324542No Hit
TGTAAACATCCCCGACTGGAAGC129090.12275585905090294No Hit
TGAGAACTGAATTCCATAGGCTGT128860.1225371446068584No Hit
CAACGGAATCCCAAAAGCAGCTGA127540.12128191388451592No Hit
ACCGGGTGCTGTAGGCTTA126230.12003619248582756No Hit
CACTAGATTGTGAGCTCCTGGAA125930.11975091277620425No Hit
TGAGGTAGTAGGTTGTATAGT124440.11833402355174188No Hit
TGAGGTAGTAGGTTGTGTGGTT122120.11612786046398843No Hit
TGTAAACATCCCCGACTGGA118850.11301831162909452No Hit
CAAAGAATTCTCCTTTTGGGCTT118570.11275205056677946No Hit
TGTAAACATCCTACACTCAGCT118390.11258088274100549No Hit
CAAAGTGCTGTTCGTGCAGGT115470.10980416023400542No Hit
AGCAGCATTGTACAGGGCTATGT114090.10849187356973829No Hit
TATTGCACTTGTCCCGGCCTGA113500.10793082347414582No Hit
CGCCTGTCTGAGCGTCGCT112630.10710351231623826No Hit
TTCACAGTGGCTAAGTTCTG111510.10603846806697798No Hit
TGTCAGTTTGTCAAATACCCCA110890.10544889000042316No Hit
ATCCCGGACGAGCCCCCA109640.10426022454365944No Hit
TGAGAACTGAATTCCATGGGTT109530.10415562198346423No Hit
CTACGCCTGTCTGAGCGTCGCT108670.1033378201492108No Hit
TGTCAGTTTGTCAAATACCCCAA107380.10211111739783064No Hit
AGCAGCATTGTACAGGGCTATG105510.10033287387451212No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)