FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8239848
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT148258817.99290472348519No Hit
CCCCCCACTGCTAAATTTGACTG7155068.68348542351752No Hit
CAACGGAATCCCAAAAGCAGCTG6725008.161558320007845No Hit
CCCCCACTGCTAAATTTGACTG3303364.009005991372656No Hit
TTCAAGTAATCCAGGATAGGCT2866023.4782437734288303No Hit
TAGCAGCACGTAAATATTGGCG2414152.929847735055307No Hit
AAGCTGCCAGTTGAAGAACTGT2099652.548165937041557No Hit
AACATTCAACGCTGTCGGTGAGT1342241.6289620876501605No Hit
AACATTCAACGCTGTCGGTGAG1081531.3125606200502726No Hit
TGAGGTAGTAGGTTGTATAGTT1023971.242704962518726No Hit
TAGCTTATCAGACTGATGTTGA910731.1052752429413746No Hit
TAGCTTATCAGACTGATGTTGACT907471.1013188592799286No Hit
TAGCTTATCAGACTGATGTTGAC904421.0976173346886982No Hit
CATAAAGTAGAAAGCACTACT827161.0038534691416638No Hit
AACCGGGCGGAAACACCA757090.9188154927129724No Hit
TATTGCACTTGTCCCGGCCTGTAA652630.7920413094998839No Hit
TGAGGTAGTAGATTGTATAGTT587390.7128650916861573No Hit
TATTGCACTTGTCCCGGCCTGTAT579580.7033867615033675No Hit
TATTGCACTTGTCCCGGCCTGT514870.6248537594382809No Hit
TGTAAACATCCTTGACTGGAAGCT494580.6002295187969486No Hit
TTCAAGTAATTCAGGATAGGTT462970.5618671606563617No Hit
TCGTACGACTCTTAGCGG429990.521842150486271No Hit
CAACGGAATCCCAAAAGCAGCTGT384740.46692608892785403No Hit
AGCAGCATTGTACAGGGCTATGA359920.4368041740575797No Hit
CCCATAAAGTAGAAAGCACTAC344940.41862422704884844No Hit
TATTGCACTTGTCCCGGCCTGTA315430.38281045961042No Hit
CCCATAAAGTAGAAAGCACT298930.362785818379174No Hit
CATTGCACTTGTCTCGGTCTGA264390.32086756940176564No Hit
TGTAAACATCCCCGACTGGAAGCT260150.3157218434126455No Hit
CAACACCAGTCGATGGGCTGT247920.3008793366091219No Hit
CGCCTGTCTGAGCGTCGCT239870.291109738917514No Hit
TGATGTAGTAGGTTGTATAGTT239570.29074565453149137No Hit
ACTGGACTTGGAGTCAGAAGGC237300.28799074934391994No Hit
TTCTCACTACTGCACTTGACTAG222720.27029624818321896No Hit
TTAATGCTAATCGTGATAGGGGTT216020.26216503022871296No Hit
TGAGGTAGTAGTTTGTACAGTT214670.260526650491611No Hit
CACCCGGCTGTGTGCACATGTGC202160.24534433159446634No Hit
CAACGGAATCCCAAAAGCAGCT196780.23881508493846004No Hit
TAGCAGCACGTAAATATTGGC192380.23347518061012776No Hit
TGTAAACATCCTACACTCTCAGCT178990.2172248808473166No Hit
GCCGGTCTGAGCGTCGCT146600.17791590330307064No Hit
TTCACAGTGGCTAAGTTCCGC145290.17632606815077173No Hit
CTTCTCACTACTGCACTTGACTAG144480.17534304030851056No Hit
AACGGAATCCCAAAAGCAGCTG142640.17310998940757158No Hit
TTCACAGTGGCTAAGTTCCG138490.1680734887342582No Hit
TGATGTAGTAGATTGTATAGTT138160.16767299590963328No Hit
TTCAAGTAATCCAGGATAGGC137880.16733318381601214No Hit
CCCCCACTGCTAAATTTGACTGGCTT136240.1653428558390883No Hit
CAAAGAATTCTCCTTTTGGGCT132050.16025781058097188No Hit
CTCCCACTGCTTCACTTGACTAG130590.15848593323566162No Hit
TGAGAACTGAATTCCATGGGTT127410.1546266387438215No Hit
CCCCCCACTGCTAAATTTGACTGG127260.15444459655081016No Hit
ACCCCCCACTGCTAAATTTGACTG125370.15215086491886742No Hit
TGAGGTAGTAGTTTGTGCTGTT123340.14968722724011413No Hit
GGAATACCGGGTGCTGTAGGCTTT119310.14479636032120982No Hit
TTCAAGTAATCCAGGATAGGCTT119230.14469927115160378No Hit
TAGCTTATCAGACTGATGTTGACA114930.13948072828527905No Hit
CCCATAAAGTAGAAAGCACTA106670.1294562715234553No Hit
CTACGCCTGTCTGAGCGTCGCT106310.1290193702602281No Hit
AGCTCGGTCTGAGGCCCCTCAGT103180.12522075649939174No Hit
ACCGGGTGCTGTAGGCTT100990.12256294048142635No Hit
GCCTGTCTGAGCGTCGCA96390.11698031322907897No Hit
TTCACAGTGGCTAAGTTCTGC95670.11610651070262461No Hit
TGAGAACTGAATTCCATAGGCTGT95590.11600942153301858No Hit
GCCTGTCTGAGCGTCGCTT94080.11417686345670454No Hit
CACTAGATTGTGAGCTCCTGGA93940.11400695740989397No Hit
TATTGCACTTGTCCCGGCCTGTT93550.11353364770806453No Hit
GGAATACCGGGTGCTGTAGGCTT93300.11323024405304564No Hit
TGTAAACATCCTACACTCAGCT92750.11256275601200409No Hit
GCCTGTCTGAGCGTCGCG92600.11238071381899277No Hit
AACATTCAACGCTGTCGGTGAGTT91820.11143409441533388No Hit
TGAGGTAGTAGGTTGTATAGT88360.10723498782987259No Hit
ATCACATTGCCAGGGATTTCC87130.1057422418471797No Hit
TTCACAGTGGCTAAGTTCTG86960.10553592736176687No Hit
TTATAAAGCAATGAGACTGATT86590.10508688995233893No Hit
CCCCCCACTGCTAAATTTGACTGGCT85870.10421308742588455No Hit
TGTCAGTTTGTCAAATACCCCA85460.1037155054316536No Hit
CAAAGAATTCTCCTTTTGGGCTT85450.10370336928545285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)