FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7106257
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG95873313.491392163272451No Hit
CAACGGAATCCCAAAAGCAGCTG6717409.452796317386214No Hit
CCCCCACTGCTAAATTTGACTG3422834.81664257287627No Hit
TTCAAGTAATCCAGGATAGGCT2713423.8183533187724565No Hit
TAGCAGCACGTAAATATTGGCG2611823.675380724339128No Hit
AAGCTGCCAGTTGAAGAACTGT2337793.2897628104359296No Hit
AACATTCAACGCTGTCGGTGAGT1097931.5450187067537806No Hit
TAGCTTATCAGACTGATGTTGAC1087981.5310169615312252No Hit
TAGCTTATCAGACTGATGTTGACT1005601.4150909543519183No Hit
AACATTCAACGCTGTCGGTGAG945021.3298421377104712No Hit
TATTGCACTTGTCCCGGCCTGTAA854091.2018844801137927No Hit
CATAAAGTAGAAAGCACTACT808931.1383348505408684No Hit
TATTGCACTTGTCCCGGCCTGTAT782951.1017755197989603No Hit
TGAGGTAGTAGGTTGTATAGTT767601.0801748374706968No Hit
TAGCTTATCAGACTGATGTTGA743551.0463314231387917No Hit
TGTAAACATCCTTGACTGGAAGCT693190.9754642985751852No Hit
TATTGCACTTGTCCCGGCCTGT510900.7189438828345217No Hit
TGAGGTAGTAGATTGTATAGTT498000.7007908664153295No Hit
CAACGGAATCCCAAAAGCAGCTGT472590.665033645701246No Hit
CCCATAAAGTAGAAAGCACTAC426020.5994998492173869No Hit
TTCAAGTAATTCAGGATAGGTT413360.5816845633362261No Hit
AGCAGCATTGTACAGGGCTATGA353180.49699863092483143No Hit
TATTGCACTTGTCCCGGCCTGTA340960.4798025176967284No Hit
TTAATGCTAATCGTGATAGGGGTT323250.45488081841115513No Hit
TGTAAACATCCCCGACTGGAAGCT319450.44953341822565657No Hit
GCCTGTCTGAGCGTCGCT316630.44556508440378667No Hit
TGTAAACATCCTACACTCTCAGCT250840.35298470066590615No Hit
TTCTCACTACTGCACTTGACTAG243080.34206474660288816No Hit
ACTGGACTTGGAGTCAGAAGGC241290.33954583967340335No Hit
CCCATAAAGTAGAAAGCACT214520.30187481257714155No Hit
ACCCCCCACTGCTAAATTTGACTG211670.29786426243801767No Hit
CATTGCACTTGTCTCGGTCTGA207690.2922635643489956No Hit
CACCCGGCTGTGTGCACATGTGC199790.2811466008054592No Hit
TAGCAGCACGTAAATATTGGC194370.2735195194882482No Hit
TCGTACGACTCTTAGCGG193610.2724500394511485No Hit
TGAGGTAGTAGTTTGTACAGTT191910.27005778147342546No Hit
CAAAGAATTCTCCTTTTGGGCT191380.26931195986860595No Hit
CAACGGAATCCCAAAAGCAGCT183250.25787133789278943No Hit
CTTCTCACTACTGCACTTGACTAG170720.24023898938639568No Hit
CCCCCACTGCTAAATTTGACTGGCTT167380.23553890606545755No Hit
TAGCTTATCAGACTGATGTTGACA162520.22869986267032No Hit
CCCCCCACTGCTAAATTTGACTGG144850.20383445180775198No Hit
TGAGAACTGAATTCCATGGGTT137820.19394176146457973No Hit
TGAGAACTGAATTCCATAGGCTGT136770.19246419036069198No Hit
AACGGAATCCCAAAAGCAGCTG135510.1906911050360267No Hit
TTCAAGTAATCCAGGATAGGC135320.19042373502675178No Hit
TGATGTAGTAGGTTGTATAGTT132590.18658205015664364No Hit
CAACACCAGTCGATGGGCTGT123530.17383272234595512No Hit
CTCCCACTGCTTCACTTGACTAG122890.17293210757787117No Hit
TGTCAGTTTGTCAAATACCCCA118730.16707811158532543No Hit
TTCAAGTAATCCAGGATAGGCTT117240.16498136782837997No Hit
AGCTCGGTCTGAGGCCCCTCAGT115500.1625328214276517No Hit
ATCACATTGCCAGGGATTTCC113600.1598591213349025No Hit
TTCACAGTGGCTAAGTTCCGC112930.15891629024956458No Hit
CAAAGAATTCTCCTTTTGGGCTT112430.158212684961999No Hit
CCCATAAAGTAGAAAGCACTA104570.14715200984146787No Hit
TGTCAGTTTGTCAAATACCCCAA103770.14602624138136294No Hit
TATTGCACTTGTCCCGGCCTGTT101310.14256450336654022No Hit
CACTAGATTGTGAGCTCCTGGAA99880.14055219224410262No Hit
TTCACAGTGGCTAAGTTCCG99230.13963750537026737No Hit
CACTAGATTGTGAGCTCCTGGA98780.13900426061145832No Hit
TGTAAACATCCCCGACTGGAAGC98160.138131790054877No Hit
GGAATACCGGGTGCTGTAGGCTTT97390.13704823791202597No Hit
TGTAAACATCCTACACTCAGCT96510.13580989260591053No Hit
TGAGGTAGTAGTTTGTGCTGTT96420.13568324365414872No Hit
CCCCCCACTGCTAAATTTGACTGGCT96410.13566917154839742No Hit
TTCACAGTGGCTAAGTTCTGC95880.1349233499435779No Hit
TATTGCACTTGTCCCGGCCTGTAG93750.13192599141854847No Hit
TCGTTTCCCGGCCAATGCACCA92380.12999811293061875No Hit
TCAGTGCACTACAGAACTTTGT89190.12550911119595026No Hit
TAGCAGCACGTAAATATTGGCGT86300.12144227263382115No Hit
TGATGTAGTAGATTGTATAGTT84010.11821976041677075No Hit
CCCCCCACTGCTAAATTTGACTGT81800.11510982504573082No Hit
AACATTCAACGCTGTCGGTGAGTT80880.11381519131661014No Hit
GGAATACCGGGTGCTGTAGGCTT80250.1129286486542775No Hit
AGCAGCATTGTACAGGGCTATGT78920.11105705858935302No Hit
TAGCAGCACATCATGGTTTACA77200.10863665640012737No Hit
CTACGCCTGTCTGAGCGTCGCT77180.10860851218862475No Hit
ACCGGGTGCTGTAGGCTT75830.10670877791219766No Hit
ATCACATTGCCAGGGATTTCCA75030.10558300945209272No Hit
TCAGTGCACTACAGAACTTTGTC73760.1037958520216761No Hit
TGTCAGTTTGTCAAATACCCC72670.10226199249478313No Hit
AACCGGGCGGAAACACCA72660.1022479203890318No Hit
GACCCGCGGGCGCTCTCCAGTCCT72430.10192426195675162No Hit
TTATAAAGCAATGAGACTGATT71680.10086885402540324No Hit
TATTGCACTTGTCCCGGCCTGTTT71410.10048890717011784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)