FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6349240
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4930737.765858590949468No Hit
CAACGGAATCCCAAAAGCAGCTG4670407.355841014042626No Hit
GCCTGTCTGAGCGTCGCT4447507.004775374690514No Hit
TTCAAGTAATCCAGGATAGGCT2418983.8098733076714693No Hit
CCCCCACTGCTAAATTTGACTG2209463.4798810566303997No Hit
TAGCAGCACGTAAATATTGGCG2005343.1583937605130696No Hit
AAGCTGCCAGTTGAAGAACTGT1754052.7626141081452267No Hit
AACATTCAACGCTGTCGGTGAGT1369012.156179322249592No Hit
TCCTGTCTGAGCGTCGCT1228981.935633241143822No Hit
AACATTCAACGCTGTCGGTGAG1104821.7400822775639289No Hit
TAGCTTATCAGACTGATGTTGAC778551.2262097510883192No Hit
TAGCTTATCAGACTGATGTTGA753081.1860947136980173No Hit
TAGCTTATCAGACTGATGTTGACT690351.0872954873339171No Hit
CATAAAGTAGAAAGCACTACT682401.0747743036961905No Hit
TGAGGTAGTAGGTTGTATAGTT641941.0110501414342503No Hit
TATTGCACTTGTCCCGGCCTGT639451.0071284122194153No Hit
TATTGCACTTGTCCCGGCCTGTAA616980.9717383497867462No Hit
TATTGCACTTGTCCCGGCCTGTAT549110.8648436663285684No Hit
TGTAAACATCCTTGACTGGAAGCT434310.6840346246164896No Hit
TTCAAGTAATTCAGGATAGGTT433510.6827746312944541No Hit
TGAGGTAGTAGATTGTATAGTT363040.5717849695396614No Hit
CAACGGAATCCCAAAAGCAGCT360040.5670599945820287No Hit
TATTGCACTTGTCCCGGCCTGTA358300.5643195091066018No Hit
AGCAGCATTGTACAGGGCTATGA338340.5328826757218187No Hit
AACCGGGCGGAAACACCA337770.5319849304798684No Hit
CGCCTGTCTGAGCGTCGCT314280.4949883765616042No Hit
TCGTACGACTCTTAGCGG309240.4870504186327813No Hit
CCCATAAAGTAGAAAGCACT295030.4646697872501276No Hit
CAACGGAATCCCAAAAGCAGCTGT267410.4211685178068556No Hit
CATTGCACTTGTCTCGGTCTGA266260.41935727740642975No Hit
CCCATAAAGTAGAAAGCACTAC227870.35889334786525634No Hit
TGTAAACATCCCCGACTGGAAGCT226420.35660960996906715No Hit
CAACACCAGTCGATGGGCTGT219760.34612016556312253No Hit
ACTGGACTTGGAGTCAGAAGGC214930.33851295588133384No Hit
CACCCGGCTGTGTGCACATGTGC204850.32263704002368787No Hit
TTCTCACTACTGCACTTGACTAG203690.3208100497067366No Hit
TCGTTTCCCGGCCAATGCACCA169540.26702408477235073No Hit
CTACGCCTGTCTGAGCGTCGCT156820.24699019095198796No Hit
ACCGGGTGCTGTAGGCTT155780.24535219963334196No Hit
TGATGTAGTAGGTTGTATAGTT153710.24209196691257537No Hit
TGTAAACATCCTACACTCTCAGCT151900.2392412320214703No Hit
TTCACAGTGGCTAAGTTCCG151060.23791823903333312No Hit
TGAGGTAGTAGTTTGTACAGTT147490.23229551883375016No Hit
CTCCCACTGCTTCACTTGACTAG143850.22656254921848912No Hit
TAGCAGCACGTAAATATTGGC142950.22514505673119933No Hit
CCCTGTCTGAGCGTCGCT139670.21997908411085423No Hit
ATCCCGGACGAGCCCCCA125330.1973937038133698No Hit
TTCAAGTAATCCAGGATAGGC123600.19466896825446825No Hit
AGCTCGGTCTGAGGCCCCTCAGT119780.18865250014174925No Hit
ACCGGGTGCTGTAGGCTTT118860.18720350782140854No Hit
CTTCTCACTACTGCACTTGACTAG118770.18706175857267957No Hit
AACGGAATCCCAAAAGCAGCTG117380.18487252017564307No Hit
TATTGCACTTGTCCCGGCCTGTT111320.17532807076122497No Hit
ACCGGGTGCTGTAGGCTTA106250.16734286308282567No Hit
CCCCCACTGCTAAATTTGACTGGCTT102670.16170439296671726No Hit
TAGCTTATCAGACTGATGTTGACA101710.1601924009802748No Hit
GGAATACCGGGTGCTGTAGGCTTT101570.15997190214891863No Hit
TTCAAGTAATCCAGGATAGGCTT100840.1588221582425613No Hit
AACATTCAACGCTGTCGGTGAGTT99310.15641242101416863No Hit
TTCACAGTGGCTAAGTTCTG98380.15494767877730248No Hit
ACCCCCCACTGCTAAATTTGACTG98350.15490042902772616No Hit
CAAAGAATTCTCCTTTTGGGCT96940.15267969079763877No Hit
CAACGGAATCCCAAAAGCAGCTGA95980.1511676988111963No Hit
TGAGGTAGTAGTTTGTGCTGTT92590.14582847710907132No Hit
ACGCCTGTCTGAGCGTCGCT89170.14044200565737No Hit
TTCACAGTGGCTAAGTTCTGC89150.14041050582431913No Hit
TGATGTAGTAGATTGTATAGTT88010.1386150153404187No Hit
GGAATACCGGGTGCTGTAGGCTT87860.13837876659253706No Hit
CCCATAAAGTAGAAAGCACTA87610.137985018679401No Hit
TACGCCTGTCTGAGCGTCGCT85440.13456728679337998No Hit
CACTAGATTGTGAGCTCCTGGA84660.1333387933043955No Hit
CCCCCCACTGCTAAATTTGACTGG79630.12541658529209795No Hit
AGAATTACTCTGAGACCT77960.12278634923234906No Hit
TTATAAAGCAATGAGACTGATT75480.11888036993403936No Hit
AGCAGCATTGTACAGGGCTATGT74660.11758887677895306No Hit
TTCACAGTGGCTAAGTTCCGC74540.11739987778064775No Hit
TGAGGTAGTAGGTTGTGTGGTT74240.11692738028488449No Hit
AGCAGCATTGTACAGGGCTATG70680.11132041000182699No Hit
CCCCCCACTGCTAAATTTGACTGGCT69710.10979266809885907No Hit
TATTGCACTTGTCCCGGCCTGTAG68060.10719393187216109No Hit
ACCACAGGGTAGAACCACGGAC68010.10711518228953387No Hit
TGTAAACATCCCCGACTGGAAGC66790.1051936924734299No Hit
CAAAGTGCTGTTCGTGCAGGT66280.10439044673063232No Hit
GGGAATACCGGGTGCTGTAGGCTTT65950.10387069948529273No Hit
TCAGTGCACTACAGAACTTTGT65900.1037919499026655No Hit
CACTAGATTGTGAGCTCCTGGAA65480.10313045340859693No Hit
TTAATGCTAATCGTGATAGGGGTT64530.1016342113386799No Hit
TGTCAGTTTGTCAAATACCCCA64100.10095696492808588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)