FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11479782
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG240622720.960563536833714No Hit
CAACGGAATCCCAAAAGCAGCTG11365979.900858744530167No Hit
CCCCCACTGCTAAATTTGACTG7136746.216790527903753No Hit
TTCAAGTAATCCAGGATAGGCT3970783.4589332793950267No Hit
TAGCAGCACGTAAATATTGGCG3891993.390299571890825No Hit
AAGCTGCCAGTTGAAGAACTGT3417582.9770425954081707No Hit
AACATTCAACGCTGTCGGTGAGT1751031.5253164215139277No Hit
GCCTGTCTGAGCGTCGCT1668181.453146061484443No Hit
AACATTCAACGCTGTCGGTGAG1449201.26239331025624No Hit
TAGCTTATCAGACTGATGTTGAC1337281.1649001697070556No Hit
TATTGCACTTGTCCCGGCCTGTAA1317691.1478353857242238No Hit
TAGCTTATCAGACTGATGTTGACT1260541.0980522103991173No Hit
TATTGCACTTGTCCCGGCCTGTAT1215191.0585479759110408No Hit
TGTAAACATCCTTGACTGGAAGCT1181471.0291745958242065No Hit
TGAGGTAGTAGGTTGTATAGTT1161901.0121272337749967No Hit
CATAAAGTAGAAAGCACTACT1045050.9103395865879682No Hit
TAGCTTATCAGACTGATGTTGA895990.7804939153025727No Hit
TGAGGTAGTAGATTGTATAGTT729340.6353256533965541No Hit
CAACGGAATCCCAAAAGCAGCTGT722830.6296548140025656No Hit
TATTGCACTTGTCCCGGCCTGT700280.6100115838436653No Hit
TTCTCACTACTGCACTTGACTAG665720.5799064825447034No Hit
CCCATAAAGTAGAAAGCACTAC623430.5430678039008058No Hit
TTCAAGTAATTCAGGATAGGTT580560.5057238891818677No Hit
TCCTGTCTGAGCGTCGCT564550.4917776313173891No Hit
TATTGCACTTGTCCCGGCCTGTA546850.47635922006184434No Hit
AGCAGCATTGTACAGGGCTATGA519000.45209917749309175No Hit
TGTAAACATCCCCGACTGGAAGCT504840.439764448488656No Hit
CTTCTCACTACTGCACTTGACTAG459890.40060865267302115No Hit
CACCCGGCTGTGTGCACATGTGC398420.34706233968554456No Hit
ACCCCCCACTGCTAAATTTGACTG376390.3278720798008185No Hit
ACTGGACTTGGAGTCAGAAGGC373550.3253981652264825No Hit
TGTAAACATCCTACACTCTCAGCT355540.30970971399979547No Hit
CCCCCCACTGCTAAATTTGACTGG352860.30737517489443617No Hit
CATTGCACTTGTCTCGGTCTGA332070.2892650748942794No Hit
CAAAGAATTCTCCTTTTGGGCT321800.280318911979339No Hit
CCCCCACTGCTAAATTTGACTGGCTT315710.27501393319141426No Hit
TAGCAGCACGTAAATATTGGC302600.2635938557021379No Hit
CTCCCACTGCTTCACTTGACTAG299740.2611025191941798No Hit
CCCATAAAGTAGAAAGCACT293520.25568429783771157No Hit
TGAGGTAGTAGTTTGTACAGTT287830.2507277577222285No Hit
TCGTACGACTCTTAGCGG258490.22516978109863062No Hit
AACGGAATCCCAAAAGCAGCTG253580.2208926963944089No Hit
TGTCAGTTTGTCAAATACCCCAA249460.2173037780682595No Hit
TGTCAGTTTGTCAAATACCCCA225880.1967633183278219No Hit
CCCCCCACTGCTAAATTTGACTGGCT216400.18850532179095386No Hit
CAACGGAATCCCAAAAGCAGCT212810.1853780847057897No Hit
CAAAGAATTCTCCTTTTGGGCTT210030.18295643593231997No Hit
AGCTCGGTCTGAGGCCCCTCAGT191770.16705021053535685No Hit
TTCAAGTAATCCAGGATAGGC185400.16150132467672296No Hit
TTCACAGTGGCTAAGTTCCGC185380.16148390274310087No Hit
TGTAAACATCCCCGACTGGAAGC177900.1549680995684413No Hit
CAACACCAGTCGATGGGCTGT176050.15335657070839848No Hit
TAGCTTATCAGACTGATGTTGACA173230.15090007806768455No Hit
TTCACAGTGGCTAAGTTCTGC168670.1469278772018493No Hit
TGAGGTAGTAGTTTGTGCTGTT165360.14404454718739432No Hit
TGATGTAGTAGGTTGTATAGTT162260.14134414747597124No Hit
AACCGGGCGGAAACACCA161490.14067340303152098No Hit
TTCAAGTAATCCAGGATAGGCTT158460.13803398008777518No Hit
CCCCCCACTGCTAAATTTGACTGT158340.1379294484860427No Hit
TATTGCACTTGTCCCGGCCTGTAG155190.13518549394056437No Hit
GACCCGCGGGCGCTCTCCAGTCCT149530.13025508672551447No Hit
ATCACATTGCCAGGGATTTCC144500.12587347041956023No Hit
TAGCAGCACGTAAATATTGGCGT138230.12041169422903676No Hit
CCCATAAAGTAGAAAGCACTA137140.11946219884663314No Hit
CACTAGATTGTGAGCTCCTGGA136650.11903536147289209No Hit
CACTAGATTGTGAGCTCCTGGAA136050.11851270346422955No Hit
CCCCCCACTGCTAAATTTGACTGTA134670.11731059004430572No Hit
TATTGCACTTGTCCCGGCCTGTT132390.1153244896113881No Hit
TGTCAGTTTGTCAAATACCCC127790.11131744487830866No Hit
CCCCCCACTGCTAAATTTGACTGA125150.1090177496401935No Hit
TGAGAACTGAATTCCATAGGCTGT124320.10829473939487702No Hit
TGTAAACATCCTACACTCAGCT122430.10664836666759003No Hit
TAGCAGCACATCATGGTTTACA117200.10209253102541493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)