FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9203171
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG161143517.509562736582858No Hit
CAACGGAATCCCAAAAGCAGCTG107049511.63180603728867No Hit
CCCCCACTGCTAAATTTGACTG5170205.617846283634195No Hit
GCCTGTCTGAGCGTCGCT3566063.874816625704336No Hit
TAGCAGCACGTAAATATTGGCG2758822.9976841677721735No Hit
TTCAAGTAATCCAGGATAGGCT2573682.7965143753169426No Hit
AACATTCAACGCTGTCGGTGAGT2034682.210846674477742No Hit
AAGCTGCCAGTTGAAGAACTGT1963502.1335037673427997No Hit
AACATTCAACGCTGTCGGTGAG1548971.6830829286992495No Hit
TATTGCACTTGTCCCGGCCTGTAA1183711.2861979854552306No Hit
TAGCTTATCAGACTGATGTTGAC1034761.1243515957706316No Hit
TAGCTTATCAGACTGATGTTGACT950851.033176499708633No Hit
TATTGCACTTGTCCCGGCCTGTAT933151.0139439982153977No Hit
TGAGGTAGTAGGTTGTATAGTT787200.8553573545466014No Hit
TGTAAACATCCTTGACTGGAAGCT661040.7182741687620495No Hit
TAGCTTATCAGACTGATGTTGA658310.7153077998876691No Hit
CAACGGAATCCCAAAAGCAGCTGT617560.6710295831730172No Hit
TATTGCACTTGTCCCGGCCTGT546320.5936214811177583No Hit
CATAAAGTAGAAAGCACTACT540340.5871237207262583No Hit
TATTGCACTTGTCCCGGCCTGTA448100.48689739656038117No Hit
AGCAGCATTGTACAGGGCTATGA426090.4629817266244428No Hit
CCCATAAAGTAGAAAGCACTAC417790.45396309598072226No Hit
TGTAAACATCCCCGACTGGAAGCT411040.44662866744516644No Hit
CACCCGGCTGTGTGCACATGTGC403460.43839237584523855No Hit
TGAGGTAGTAGATTGTATAGTT397820.43226405333552964No Hit
TCGTACGACTCTTAGCGG385920.41933372747284603No Hit
ACTGGACTTGGAGTCAGAAGGC344420.37424057425424345No Hit
TTCAAGTAATTCAGGATAGGTT341400.3709590965983355No Hit
CAACGGAATCCCAAAAGCAGCT330590.35921314512139346No Hit
TTCTCACTACTGCACTTGACTAG312660.3397307297669466No Hit
CCCCCACTGCTAAATTTGACTGGCTT291170.3163800824737474No Hit
ACCCCCCACTGCTAAATTTGACTG271700.2952243308311885No Hit
CCCCCCACTGCTAAATTTGACTGG263110.2858905914059404No Hit
CATTGCACTTGTCTCGGTCTGA258570.2809575091020258No Hit
CAAAGAATTCTCCTTTTGGGCT256600.27881694255164874No Hit
CTTCTCACTACTGCACTTGACTAG253450.27539420923505603No Hit
TGTAAACATCCTACACTCTCAGCT250410.2720909999390428No Hit
CCCATAAAGTAGAAAGCACT249480.271080478674144No Hit
TCCTGTCTGAGCGTCGCT237520.2580849578911443No Hit
TAGCAGCACGTAAATATTGGC228170.24792541614189284No Hit
AACGGAATCCCAAAAGCAGCTG221850.24105821786860204No Hit
TTAATGCTAATCGTGATAGGGGTT211440.229746899193767No Hit
AACCGGGCGGAAACACCA189730.20615720385940892No Hit
TGAGAACTGAATTCCATGGGTT188400.2047120497924031No Hit
GACCCGCGGGCGCTCTCCAGTCCT187450.20367979688739893No Hit
CCCCCCACTGCTAAATTTGACTGGCT162870.17697161119792296No Hit
TAGCTTATCAGACTGATGTTGACA161130.17508095850875746No Hit
CAAAGAATTCTCCTTTTGGGCTT160320.17420082708449078No Hit
TGAGGTAGTAGTTTGTACAGTT153910.1672358364307259No Hit
CTCCCACTGCTTCACTTGACTAG148480.1613356961421232No Hit
CACTAGATTGTGAGCTCCTGGA147740.16053162545822522No Hit
CACTAGATTGTGAGCTCCTGGAA142240.15455542442925377No Hit
AACATTCAACGCTGTCGGTGAGTT140470.15263217427993025No Hit
CAACACCAGTCGATGGGCTGT132560.14403731061826408No Hit
TGATGTAGTAGGTTGTATAGTT132290.14374393347684186No Hit
TATTGCACTTGTCCCGGCCTGTAG130670.14198367062830844No Hit
TGTAAACATCCCCGACTGGAAGC121390.13190018961942573No Hit
ATCACATTGCCAGGGATTTCC121020.13149815427747677No Hit
TCGTTTCCCGGCCAATGCACCA120500.13093313163473763No Hit
TTCACAGTGGCTAAGTTCCGC119270.12959663576825858No Hit
AGCTCGGTCTGAGGCCCCTCAGT117100.12723875281682803No Hit
CCCATAAAGTAGAAAGCACTA116620.1267171934542996No Hit
TGAGAACTGAATTCCATAGGCTGT116600.12669546181419425No Hit
TGTCAGTTTGTCAAATACCCCA110380.11993692174143021No Hit
GGAATACCGGGTGCTGTAGGCTTT110140.119676142060166No Hit
TGAGGTAGTAGTTTGTGCTGTT108340.11772029445068445No Hit
TATTGCACTTGTCCCGGCCTGTAAT107460.116764102286049No Hit
TTCAAGTAATCCAGGATAGGCTT106530.11575358102115021No Hit
TTCAAGTAATCCAGGATAGGC105360.1144822800749872No Hit
TATTGCACTTGTCCCGGCCTGTT105090.11418890293356497No Hit
TATTGCACTTGTCCCGGCCTGTAAA103450.11240690844492622No Hit
ACTGGACTTGGAGTCAGAAGGCA102940.11185275162223977No Hit
CCCCCCACTGCTAAATTTGACTGT100780.1095057344908619No Hit
ACTGGACTTGGAGTCAGAAGGCT99360.10796278804338201No Hit
ATGCACCTGGGCAAGGATTCTGA97190.10560490509195146No Hit
TTCACAGTGGCTAAGTTCTGC96800.10518113810989711No Hit
CGCCTGTCTGAGCGTCGCT95430.10369252076268061No Hit
CCCCCCACTGCTAAATTTGACTGTA94820.1030297057394674No Hit
TGTCAGTTTGTCAAATACCCCAA93950.10208437939488466No Hit
CTCCCACTGCTTCACTTGACTAGCCT92560.1005740304075628No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)