FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7688663
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG111455014.496018358458421No Hit
CAACGGAATCCCAAAAGCAGCTG7254449.435242512254732No Hit
GCCTGTCTGAGCGTCGCT4097015.328637761857946No Hit
CCCCCACTGCTAAATTTGACTG3636644.729873061155105No Hit
TAGCAGCACGTAAATATTGGCG2623993.4128040206730352No Hit
TTCAAGTAATCCAGGATAGGCT2504073.25683412057467No Hit
AAGCTGCCAGTTGAAGAACTGT2337983.040814768445437No Hit
AACATTCAACGCTGTCGGTGAGT1404931.8272747810640158No Hit
AACATTCAACGCTGTCGGTGAG1121371.4584720386366263No Hit
TATTGCACTTGTCCCGGCCTGTAA931481.211498019876798No Hit
TGAGGTAGTAGGTTGTATAGTT816051.061367886718406No Hit
TATTGCACTTGTCCCGGCCTGTAT806401.0488169399543197No Hit
CATAAAGTAGAAAGCACTACT688030.8948629950356779No Hit
TATTGCACTTGTCCCGGCCTGT645220.8391836135879541No Hit
TAGCTTATCAGACTGATGTTGAC636050.8272569626214596No Hit
TAGCTTATCAGACTGATGTTGACT582490.7575959565401683No Hit
TGTAAACATCCTTGACTGGAAGCT544790.7085627241043079No Hit
TGAGGTAGTAGATTGTATAGTT501440.6521810098842933No Hit
TAGCTTATCAGACTGATGTTGA500170.6505292272531649No Hit
CAACGGAATCCCAAAAGCAGCTGT480670.6251672104759957No Hit
TATTGCACTTGTCCCGGCCTGTA429960.5592129606929059No Hit
CCCATAAAGTAGAAAGCACTAC393720.5120786279747207No Hit
CACCCGGCTGTGTGCACATGTGC353410.45965078713945456No Hit
TTCAAGTAATTCAGGATAGGTT333740.43406766560063825No Hit
TTCTCACTACTGCACTTGACTAG323560.42082739222671095No Hit
AGCAGCATTGTACAGGGCTATGA318850.4147014897128408No Hit
TGAGAACTGAATTCCATGGGTT299810.38993775640836387No Hit
TGTAAACATCCCCGACTGGAAGCT289140.3760601810743949No Hit
AACCGGGCGGAAACACCA285470.3712869194553071No Hit
TCGTACGACTCTTAGCGG271600.35324737213739243No Hit
ACTGGACTTGGAGTCAGAAGGC261460.34005912341326444No Hit
CATTGCACTTGTCTCGGTCTGA242080.3148531805854932No Hit
CCCATAAAGTAGAAAGCACT219400.28535520414927795No Hit
TGAGGTAGTAGTTTGTACAGTT209810.27288229435989064No Hit
CGCCTGTCTGAGCGTCGCT205630.26744571845586157No Hit
TCCTGTCTGAGCGTCGCT202170.26294558624822023No Hit
CTTCTCACTACTGCACTTGACTAG197620.25702778233354745No Hit
TGTAAACATCCTACACTCTCAGCT195880.25476471006727697No Hit
CTACGCCTGTCTGAGCGTCGCT189690.246713895510832No Hit
AACGGAATCCCAAAAGCAGCTG183690.23891019804093377No Hit
CCCCCCACTGCTAAATTTGACTGG182540.23741448935920328No Hit
ACCCCCCACTGCTAAATTTGACTG176870.23003999525014945No Hit
TAGCAGCACGTAAATATTGGC176070.22899950225416305No Hit
TTAATGCTAATCGTGATAGGGGTT173650.2258520109413041No Hit
CAACGGAATCCCAAAAGCAGCT168080.2186075784567486No Hit
CTCCCACTGCTTCACTTGACTAG154190.20054201881393421No Hit
CAAAGAATTCTCCTTTTGGGCT143440.1865603941803666No Hit
GACCCGCGGGCGCTCTCCAGTCCT143220.18627425860647034No Hit
CAACACCAGTCGATGGGCTGT137930.17939399867051006No Hit
AGCTCGGTCTGAGGCCCCTCAGT133810.17403545974117995No Hit
GGAATACCGGGTGCTGTAGGCTTT126300.16426783174135737No Hit
TTCAAGTAATCCAGGATAGGC122870.15980671802106555No Hit
TGAGGTAGTAGTTTGTGCTGTT121700.1582849970144354No Hit
ATCCCGGACGAGCCCCCA118620.15427909897988765No Hit
CCCCCACTGCTAAATTTGACTGGCTT118430.15403198189334089No Hit
TTCACAGTGGCTAAGTTCCGC115310.14997405920899382No Hit
TACGCCTGTCTGAGCGTCGCT112510.14633233372304133No Hit
GGAATACCGGGTGCTGTAGGCTT110230.14336692868447998No Hit
TATTGCACTTGTCCCGGCCTGTAG109620.14257355277504036No Hit
TTCACAGTGGCTAAGTTCTGC109600.14254754045014068No Hit
TGAGAACTGAATTCCATAGGCTGT108550.1411818933929085No Hit
TGTCAGTTTGTCAAATACCCCA108230.14076569619451393No Hit
TATTGCACTTGTCCCGGCCTGTT106140.13804740824249936No Hit
TGTCAGTTTGTCAAATACCCCAA105840.13765722336900446No Hit
TGTAAACATCCCCGACTGGAAGC105520.1372410261706099No Hit
CAAAGAATTCTCCTTTTGGGCTT98430.12801965699368018No Hit
TCGTTTCCCGGCCAATGCACCA97960.12740836735853817No Hit
CCCATAAAGTAGAAAGCACTA90710.11797889958241115No Hit
TTCAAGTAATCCAGGATAGGCTT89160.11596294440268744No Hit
CCCCCCACTGCTAAATTTGACTGT88350.11490944524425119No Hit
AACATTCAACGCTGTCGGTGAGTT88120.11461030350790509No Hit
TGAGGTAGTAGGTTGTGTGGTT87590.11392097689806406No Hit
GGGAATACCGGGTGCTGTAGGCTTT87390.11366085364906747No Hit
CACTAGATTGTGAGCTCCTGGA86820.11291950238942712No Hit
TAGCAGCACGTAAATATTGGCGT84970.11051336233620851No Hit
ACGCCTGTCTGAGCGTCGCT84270.1096029309647204No Hit
ACCGGGTGCTGTAGGCTT82780.10766501275969566No Hit
GGGAATACCGGGTGCTGTAGGCTT82470.10726182172375093No Hit
CCCCCCACTGCTAAATTTGACTGGCT81920.10654648278901026No Hit
TTCACAGTGGCTAAGTTCCG81900.1065204704641106No Hit
AATGACACGATCACTCCCGTTG81620.10615629791551535No Hit
ATCACATTGCCAGGGATTTCC80860.10516782956932824No Hit
TAGCTTATCAGACTGATGTTGACA79550.10346402228840047No Hit
CACTAGATTGTGAGCTCCTGGAA78520.10212438755606792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)