FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5615552
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG77608413.820262015203491No Hit
CAACGGAATCCCAAAAGCAGCTG3467386.174602247472733No Hit
CCCCCACTGCTAAATTTGACTG2328804.147054465883318No Hit
AAGCTGCCAGTTGAAGAACTGT1786883.182020218137059No Hit
TAGCAGCACGTAAATATTGGCG1714443.0530213236383528No Hit
GCCTGTCTGAGCGTCGCT1537972.7387690471034727No Hit
AACATTCAACGCTGTCGGTGAGT1513192.6946415953409386No Hit
TTCAAGTAATCCAGGATAGGCT1511592.691792365202922No Hit
TCGTACGACTCTTAGCGG814071.4496704865345382No Hit
AACATTCAACGCTGTCGGTGAG731461.302561172971063No Hit
TAGCTTATCAGACTGATGTTGAC680991.2126857698049986No Hit
TATTGCACTTGTCCCGGCCTGT606821.0806061452195617No Hit
CGCCTGTCTGAGCGTCGCT521860.9293120248908745No Hit
TGAGGTAGTAGGTTGTATAGTT451050.8032157835952726No Hit
TAGCTTATCAGACTGATGTTGA442250.7875450178361807No Hit
TATTGCACTTGTCCCGGCCTGTAA430230.7661401764243302No Hit
TATTGCACTTGTCCCGGCCTGTAT425200.7571829091779402No Hit
CATAAAGTAGAAAGCACTACT410320.7306850688943848No Hit
TAGCTTATCAGACTGATGTTGACT400440.7130910727921316No Hit
TGTAAACATCCTTGACTGGAAGCT370360.6595255461974174No Hit
TTCAAGTAATTCAGGATAGGTT340390.606155904174692No Hit
AGCAGCATTGTACAGGGCTATGA335530.5975013676304662No Hit
TATTGCACTTGTCCCGGCCTGTA323100.5753664109957489No Hit
TGAGGTAGTAGATTGTATAGTT275860.4912428911708056No Hit
TTAATGCTAATCGTGATAGGGGTT264080.4702654342796576No Hit
ACTGGACTTGGAGTCAGAAGGC236150.42052856068290345No Hit
CAACGGAATCCCAAAAGCAGCTGT212170.3778257239893781No Hit
CATTGCACTTGTCTCGGTCTGA206160.3671233032834528No Hit
TGTAAACATCCCCGACTGGAAGCT199550.35535242127577127No Hit
TGTAAACATCCTACACTCTCAGCT199040.3544442291692785No Hit
CCCATAAAGTAGAAAGCACTAC193840.34518423122072417No Hit
GCGTTCGCGCTTTCCCCTG190840.33984192471194286No Hit
TTCTCACTACTGCACTTGACTAG174440.31063731579727155No Hit
CCCATAAAGTAGAAAGCACT166990.2973705879671313No Hit
CACCCGGCTGTGTGCACATGTGC158580.2823943220541809No Hit
TACGCCTGTCTGAGCGTCGCT156440.2785834767445836No Hit
CTACGCCTGTCTGAGCGTCGCT152130.2709083630603011No Hit
AACCGGGCGGAAACACCA149270.26581536418859625No Hit
ACCGGGTGCTGTAGGCTT135960.24211333097796972No Hit
AACATTCAACGCTGTCGGTGAGTT135950.24209552328960715No Hit
CCCCCCACTGCTAAATTTGACTGG130000.2314999487138575No Hit
CTCCCACTGCTTCACTTGACTAG128690.22916714153835635No Hit
CAACGGAATCCCAAAAGCAGCT127430.22692337280466818No Hit
ACGCCTGTCTGAGCGTCGCT125830.22407414266665146No Hit
GACCCGCGGGCGCTCTCCAGTCCT121980.21721818264704876No Hit
ATCCCGGACGAGCCCCCA121850.21698668269833488No Hit
CTTCTCACTACTGCACTTGACTAG120400.2144045678857573No Hit
TGAGGTAGTAGTTTGTACAGTT104130.18543145891979987No Hit
TGAGGTAGTAGTTTGTGCTGTT103240.1838465746555281No Hit
CAACACCAGTCGATGGGCTGT101600.18092611376406095No Hit
TGTCAGTTTGTCAAATACCCCAA96540.17191542345258312No Hit
AGCTCGGTCTGAGGCCCCTCAGT95400.1698853469792462No Hit
TTCACAGTGGCTAAGTTCCG94420.168140193519711No Hit
TGTAAACATCCTACACTCAGCT91050.16213900254151328No Hit
AACGGAATCCCAAAAGCAGCTG90150.16053631058887888No Hit
ACCCCCCACTGCTAAATTTGACTG89970.160215772198352No Hit
TATTGCACTTGTCCCGGCCTGTT87140.15517619639173497No Hit
TGTCAGTTTGTCAAATACCCCA84900.15118727419851155No Hit
TAGCAGCACGTAAATATTGGC83040.14787504416306713No Hit
TTCAAGTAATCCAGGATAGGC82730.1473230058238264No Hit
TTCAAGTAATCCAGGATAGGCTT82160.14630796758715794No Hit
ACCACAGGGTAGAACCACGGAC81680.14545319854575295No Hit
TGAGGTAGTAGGTTGTGTGGTT79960.14239027614738498No Hit
AGCAGCATTGTACAGGGCTATG78910.14052046886931152No Hit
ACCGGGTGCTGTAGGCTTT77590.13816985400544773No Hit
AATGACACGATCACTCCCGTTG74860.13330835508245673No Hit
CACTAGATTGTGAGCTCCTGGA74630.1328987782501168No Hit
ATCCCACCGCTGCCACCA73960.13170566312982232No Hit
CCCCCCACTGCTAAATTTGACTGT73860.1315275862461963No Hit
TTATAAAGCAATGAGACTGATT73170.13029885574917657No Hit
AGCAGCATTGTACAGGGCTATGT72690.12944408670777155No Hit
CAAAGAATTCTCCTTTTGGGCT72640.12935504826595853No Hit
TCGTTTCCCGGCCAATGCACCA71650.12759208711806072No Hit
TACCTGGTTGATCCTGCC71490.12730716410425902No Hit
TAGCAGCACGTAAATATTGGCGT71400.12714689490899558No Hit
GGGCTACGCCTGTCTGAGCGTCGCT71310.12698662571373215No Hit
CAAAGTGCTGTTCGTGCAGGTAG68460.12191143453038988No Hit
TGATGTAGTAGGTTGTATAGTT67850.12082516554027102No Hit
CAAAGTGCTGTTCGTGCAGGT66260.11799374309061691No Hit
CCTCGACACAAGGGTTTGT66020.1175663585699144No Hit
CCCCCCACTGCTAAATTTGACTGA65690.11697870485394846No Hit
CCCCCCACTGCTAAATTTGACTGGCT65500.11664035877505899No Hit
ACCGGGTGCTGTAGGCTTA65400.11646228189143293No Hit
CCCCCCACTGCTAAATTTGACTGTA64810.11541162827803927No Hit
TTCACCACCTTCTCCACCCAGC64490.11484178225043594No Hit
CTCCCACTGCTTCACTTGACTA62800.11183228291715577No Hit
TAGCTTATCAGACTGATGTTGACA61440.10941043729984158No Hit
GGAATACCGGGTGCTGTAGGCTTT59970.10679270711053875No Hit
GGAATACCGGGTGCTGTAGGCTT59410.10579547656223288No Hit
CAAAGAATTCTCCTTTTGGGCTT58550.1042640153630489No Hit
TGTAAACATCCCCGACTGGAAGC57290.10202024662936074No Hit
TATTGCACTTGTCCCGGCCTGTAG56870.10127232371813136No Hit
TCGTTTCCCGGGCGGCGCACCA56850.10123670834140616No Hit
CTCGACACAAGGGTTTGT56320.10029290085818811No Hit
CACTAGATTGTGAGCTCCTGGAA56230.10013263166292467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)