FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10010242
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG162716516.255001627333286No Hit
CAACGGAATCCCAAAAGCAGCTG8932818.923670376800082No Hit
CCCCCACTGCTAAATTTGACTG5448575.442995284229892No Hit
TTCAAGTAATCCAGGATAGGCT3277573.2742165474121405No Hit
TAGCAGCACGTAAATATTGGCG3212063.2087735741054018No Hit
GCCTGTCTGAGCGTCGCT2363162.360742127912592No Hit
AAGCTGCCAGTTGAAGAACTGT2325502.323120659820212No Hit
AACATTCAACGCTGTCGGTGAGT2040102.038012667426022No Hit
AACATTCAACGCTGTCGGTGAG1655101.6534065809797605No Hit
TATTGCACTTGTCCCGGCCTGTAA1225561.224306065727482No Hit
TAGCTTATCAGACTGATGTTGAC1181801.1805908388628366No Hit
TATTGCACTTGTCCCGGCCTGTAT1100601.0994739188123523No Hit
TAGCTTATCAGACTGATGTTGACT1029231.0281769411768467No Hit
TGAGGTAGTAGGTTGTATAGTT1017351.0163090962236478No Hit
TAGCTTATCAGACTGATGTTGA921680.9207369811838715No Hit
TGTAAACATCCTTGACTGGAAGCT828490.8276423287269179No Hit
CATAAAGTAGAAAGCACTACT803850.8030275391943571No Hit
TATTGCACTTGTCCCGGCCTGT759260.7584831615459446No Hit
TATTGCACTTGTCCCGGCCTGTA620660.6200249704252904No Hit
TGAGGTAGTAGATTGTATAGTT603330.602712701650969No Hit
TTCAAGTAATTCAGGATAGGTT594500.5938917360839029No Hit
AGCAGCATTGTACAGGGCTATGA540610.5400568737499054No Hit
CAACGGAATCCCAAAAGCAGCT508040.5075201978133995No Hit
TTCTCACTACTGCACTTGACTAG494240.4937343173122088No Hit
CAACGGAATCCCAAAAGCAGCTGT479950.4794589381555411No Hit
CCCATAAAGTAGAAAGCACTAC443650.44319607857632215No Hit
CACCCGGCTGTGTGCACATGTGC438690.43824115341067676No Hit
TGTAAACATCCCCGACTGGAAGCT418010.41758231219584896No Hit
ACTGGACTTGGAGTCAGAAGGC341420.34107067541424074No Hit
CATTGCACTTGTCTCGGTCTGA322860.32252966511698716No Hit
CCCCCACTGCTAAATTTGACTGGCTT321740.32141081104732533No Hit
CTTCTCACTACTGCACTTGACTAG319960.3196326322580413No Hit
CCCATAAAGTAGAAAGCACT305830.30551708939703953No Hit
ACCCCCCACTGCTAAATTTGACTG276970.2766866175662886No Hit
TAGCAGCACGTAAATATTGGC272040.2717616617060806No Hit
TGTAAACATCCTACACTCTCAGCT259890.25962409300394534No Hit
CCCCCCACTGCTAAATTTGACTGG253480.25322065140882705No Hit
GACCCGCGGGCGCTCTCCAGTCCT250960.2507032297520879No Hit
AACCGGGCGGAAACACCA250100.24984410966288326No Hit
CTCCCACTGCTTCACTTGACTAG243960.24371039181670132No Hit
TGAGGTAGTAGTTTGTACAGTT239020.23877544618801425No Hit
GGAATACCGGGTGCTGTAGGCTTT233900.23366068472670293No Hit
CAAAGAATTCTCCTTTTGGGCT224470.22424033305088928No Hit
TCGTTTCCCGGCCAATGCACCA221760.22153310579304678No Hit
CGCCTGTCTGAGCGTCGCT215150.21492986882834603No Hit
AACGGAATCCCAAAAGCAGCTG214990.21477003253268004No Hit
CTACGCCTGTCTGAGCGTCGCT206790.2065784223797986No Hit
AGCTCGGTCTGAGGCCCCTCAGT197990.19778742611816974No Hit
GGAATACCGGGTGCTGTAGGCTT192750.19255278743510895No Hit
GGGAATACCGGGTGCTGTAGGCTTT192110.19191344225244503No Hit
CCCCCCACTGCTAAATTTGACTGGCT185670.1854800313518894No Hit
CAACACCAGTCGATGGGCTGT169260.16908682127764743No Hit
GGGAATACCGGGTGCTGTAGGCTT168920.16874716914935722No Hit
TATTGCACTTGTCCCGGCCTGTAC168710.16853738401129564No Hit
TCGTACGACTCTTAGCGG162390.16222385033248946No Hit
TAGCTTATCAGACTGATGTTGACA156970.15680939581680442No Hit
TATTGCACTTGTCCCGGCCTGTAG151310.15115518685762044No Hit
TGATGTAGTAGGTTGTATAGTT148930.148777621959589No Hit
TTCACAGTGGCTAAGTTCTGC148690.14853786751609002No Hit
CAAAGAATTCTCCTTTTGGGCTT148180.14802838932365472No Hit
TATTGCACTTGTCCCGGCCTGTT146880.14672971942136864No Hit
TGTAAACATCCCCGACTGGAAGC145840.1456907834995398No Hit
TGTCAGTTTGTCAAATACCCCA145650.1455009778984364No Hit
TGAGGTAGTAGTTTGTGCTGTT144310.14416234892223387No Hit
TTCAAGTAATCCAGGATAGGC142900.14275379156667742No Hit
CAACGGAATCCCAAAAGCAGCTGA142720.14257397573405317No Hit
TGTCAGTTTGTCAAATACCCCAA141450.14130527513720448No Hit
AACATTCAACGCTGTCGGTGAGTT141340.14119538768393414No Hit
TTCAAGTAATCCAGGATAGGCTT139130.13898764885004777No Hit
CACTAGATTGTGAGCTCCTGGA128130.12799890352301171No Hit
TTCACAGTGGCTAAGTTCCG126670.12654039732505967No Hit
CACTAGATTGTGAGCTCCTGGAA124740.12461237200858881No Hit
CCCCCCACTGCTAAATTTGACTGTA118770.11864848022655197No Hit
CCCATAAAGTAGAAAGCACTA115000.11488233750992234No Hit
CCCCCCACTGCTAAATTTGACTGT114790.11467255237186075No Hit
TTCACAGTGGCTAAGTTCCGC114290.11417306394790455No Hit
AATGCACCCGGGCAAGGATTCT114280.11416307417942544No Hit
TACGCCTGTCTGAGCGTCGCT113350.11323402571086694No Hit
ATCACATTGCCAGGGATTTCC108410.10829908008217982No Hit
TGAGGTAGTAGGTTGTGTGGTT105370.10526219046452623No Hit
TAGCAGCACGTAAATATTGGCGT104870.10476270204057006No Hit
AGCAGCATTGTACAGGGCTATGT104740.10463283505034145No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC104480.10437310106988422No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)