FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8893914
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG192873421.685997863257953No Hit
CAACGGAATCCCAAAAGCAGCTG7384228.302553858739808No Hit
CCCCCACTGCTAAATTTGACTG6938217.801076106650008No Hit
TAGCAGCACGTAAATATTGGCG2748013.089764528867718No Hit
TTCAAGTAATCCAGGATAGGCT2533712.8488132446524665No Hit
AAGCTGCCAGTTGAAGAACTGT2064982.3217899341055017No Hit
AACATTCAACGCTGTCGGTGAGT1271931.430112771497453No Hit
AACATTCAACGCTGTCGGTGAG1175051.321184351456513No Hit
GCCTGTCTGAGCGTCGCT1152691.2960435641720844No Hit
TAGCTTATCAGACTGATGTTGAC1053641.1846752734510362No Hit
CATAAAGTAGAAAGCACTACT806960.9073170709768499No Hit
TAGCTTATCAGACTGATGTTGACT777550.8742495148929931No Hit
TATTGCACTTGTCCCGGCCTGTAT745800.8385509461863472No Hit
TAGCTTATCAGACTGATGTTGA709540.7977814941768046No Hit
TATTGCACTTGTCCCGGCCTGTAA692980.779162020230913No Hit
TATTGCACTTGTCCCGGCCTGT666100.7489391059999007No Hit
TGTAAACATCCTTGACTGGAAGCT627690.7057522705976244No Hit
TGAGGTAGTAGGTTGTATAGTT618370.6952731946812168No Hit
TATTGCACTTGTCCCGGCCTGTA456270.5130137305127979No Hit
CAACGGAATCCCAAAAGCAGCTGT401780.4517471160616125No Hit
AGCAGCATTGTACAGGGCTATGA386950.43507279247359487No Hit
TCCTGTCTGAGCGTCGCT385080.4329702311041011No Hit
TTCAAGTAATTCAGGATAGGTT377520.4244700364766289No Hit
TTCTCACTACTGCACTTGACTAG373680.42015247730076993No Hit
CCCATAAAGTAGAAAGCACTAC372800.4191630366563023No Hit
TGAGGTAGTAGATTGTATAGTT350440.39402224937187386No Hit
CATTGCACTTGTCTCGGTCTGA316640.35601873370936576No Hit
ACCCCCCACTGCTAAATTTGACTG309820.34835056871474135No Hit
TCGTACGACTCTTAGCGG304670.3425600922158681No Hit
TGTAAACATCCCCGACTGGAAGCT297080.3340261666573344No Hit
CTTCTCACTACTGCACTTGACTAG276380.31075182422497No Hit
ACTGGACTTGGAGTCAGAAGGC249850.28092243752300733No Hit
CACCCGGCTGTGTGCACATGTGC226260.2543986820650616No Hit
CCCATAAAGTAGAAAGCACT223980.25183513130439533No Hit
CCCCCCACTGCTAAATTTGACTGG222810.25051962499300084No Hit
TAGCAGCACGTAAATATTGGC222300.24994619916495708No Hit
CTCCCACTGCTTCACTTGACTAG216860.24382965699915696No Hit
CAACGGAATCCCAAAAGCAGCT206250.23190015104710932No Hit
GACCCGCGGGCGCTCTCCAGTCCT190890.21462991434367368No Hit
TGTAAACATCCTACACTCTCAGCT189440.21299958600903943No Hit
TGATGTAGTAGGTTGTATAGTT189210.21274098220423537No Hit
CCCCCACTGCTAAATTTGACTGGCTT182850.20559002481921906No Hit
CAAAGAATTCTCCTTTTGGGCT172610.19407653368359531No Hit
TGTCAGTTTGTCAAATACCCCAA170440.19163666300348756No Hit
TGTCAGTTTGTCAAATACCCCA168960.18997260373779193No Hit
CAACACCAGTCGATGGGCTGT156510.17597426734731186No Hit
TGAGGTAGTAGTTTGTACAGTT145780.16390983767101863No Hit
AACGGAATCCCAAAAGCAGCTG141070.1586140814943792No Hit
CGCCCCACTGCTAAATTTGACTG133660.15028254152221396No Hit
TAGCTTATCAGACTGATGTTGACA131450.14779769626735764No Hit
TTCACAGTGGCTAAGTTCCGC130940.1472242704393139No Hit
CCCCCCACTGCTAAATTTGACTGT126560.14229955450434983No Hit
TTCAAGTAATCCAGGATAGGC125730.14136633207831784No Hit
TATTGCACTTGTCCCGGCCTGTT119620.13449646578547983No Hit
CACTAGATTGTGAGCTCCTGGA116430.13090974344928452No Hit
TGATGTAGTAGATTGTATAGTT108530.1220272649364498No Hit
CCCATAAAGTAGAAAGCACTA108390.12186985392482995No Hit
TAGCAGCACGTAAATATTGGCGT107600.12098160607354647No Hit
CCCGCCACTGCTAAATTTGACTG106550.11980102348639755No Hit
TTCAAGTAATCCAGGATAGGCTT106220.11942998324472219No Hit
ACCGGGTGCTGTAGGCTT105680.11882282648561701No Hit
CTCCCCACTGCTAAATTTGACTG105320.11841805531288024No Hit
CCCCCCACTGCTAAATTTGACTGA104080.1170238434956758No Hit
CAAAGAATTCTCCTTTTGGGCTT100330.1128074771130011No Hit
TGAGGTAGTAGTTTGTGCTGTT100180.1126388224576941No Hit
TGTAAACATCCCCGACTGGAAGC99910.11233524407814152No Hit
CCCCCCACTGCTAAATTTGACTGTA98860.11115466149099261No Hit
CTCCCACTGCTTCACTTGACTA98720.11099725047937276No Hit
CCCCCCACTGCTAAATTTGACTGGCT98560.11081735218037862No Hit
TTCACAGTGGCTAAGTTCTGC95970.10790524846541129No Hit
ATCACATTGCCAGGGATTTCC95930.10786027389066276No Hit
CCCCCACAACCGCGCTTGACTAG94730.10651103664820687No Hit
CACTAGATTGTGAGCTCCTGGAA94090.10579144345223036No Hit
AGCTCGGTCTGAGGCCCCTCAGT91810.10322789269156414No Hit
CTTCTCACTACTGCACTTGACTA91450.10282312151882737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)