FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7773874
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG140230918.03874104468377No Hit
GCCTGTCTGAGCGTCGCT6371158.195592056161445No Hit
CAACGGAATCCCAAAAGCAGCTG5734877.377106961085297No Hit
CCCCCACTGCTAAATTTGACTG5146836.6206758689425635No Hit
TTCAAGTAATCCAGGATAGGCT1992222.5627119760366583No Hit
TAGCAGCACGTAAATATTGGCG1960842.522346001491663No Hit
AAGCTGCCAGTTGAAGAACTGT1777402.2863761362738835No Hit
AACATTCAACGCTGTCGGTGAGT1229701.5818368036322688No Hit
AACATTCAACGCTGTCGGTGAG1058761.3619464375162242No Hit
TGAGGTAGTAGGTTGTATAGTT660740.8499494589184234No Hit
TAGCTTATCAGACTGATGTTGAC643210.8273995693781504No Hit
TATTGCACTTGTCCCGGCCTGTAT611550.7866734140532764No Hit
TATTGCACTTGTCCCGGCCTGTAA595160.7655899748310817No Hit
TATTGCACTTGTCCCGGCCTGT584790.7522504223762825No Hit
TAGCTTATCAGACTGATGTTGACT558360.7182519294755743No Hit
CATAAAGTAGAAAGCACTACT537420.6913155525803479No Hit
TAGCTTATCAGACTGATGTTGA478210.6151501812352503No Hit
TGTAAACATCCTTGACTGGAAGCT445680.5733048927728954No Hit
TATTGCACTTGTCCCGGCCTGTA384050.4940265304017019No Hit
TCCTGTCTGAGCGTCGCT352440.4533646930732348No Hit
TGAGGTAGTAGATTGTATAGTT350490.4508562912133642No Hit
CCCATAAAGTAGAAAGCACTAC333810.42939980761200913No Hit
CAACGGAATCCCAAAAGCAGCTGT331980.42704576894351515No Hit
AGCAGCATTGTACAGGGCTATGA297500.3826920786212897No Hit
GACCCGCGGGCGCTCTCCAGTCCT291850.37542414502730553No Hit
TTCAAGTAATTCAGGATAGGTT276280.3553955209461846No Hit
TTCTCACTACTGCACTTGACTAG253610.3262337413752783No Hit
TCGTACGACTCTTAGCGG249460.3208953476735023No Hit
ACTGGACTTGGAGTCAGAAGGC243530.31326723329963924No Hit
TGTAAACATCCCCGACTGGAAGCT241940.3112219210138986No Hit
ACCCCCCACTGCTAAATTTGACTG222540.2862665384080061No Hit
CATTGCACTTGTCTCGGTCTGA217240.2794488307888705No Hit
CACCCGGCTGTGTGCACATGTGC210720.2710617640574056No Hit
AACCGGGCGGAAACACCA198590.2554582181290821No Hit
CCCATAAAGTAGAAAGCACT169520.2180637350180875No Hit
CCCCCCACTGCTAAATTTGACTGG163100.20980530427943647No Hit
CGCCTGTCTGAGCGTCGCT162130.20855753514914185No Hit
CTTCTCACTACTGCACTTGACTAG161500.20774712839441442No Hit
GGAATACCGGGTGCTGTAGGCTTT150110.1930954888129136No Hit
ACCGGGTGCTGTAGGCTT147510.18975095299975275No Hit
CTCCCACTGCTTCACTTGACTAG147130.18926213622706No Hit
CAACGGAATCCCAAAAGCAGCT145580.1872682783384449No Hit
TAGCAGCACGTAAATATTGGC144930.18643214438515468No Hit
TGAGAACTGAATTCCATGGGTT143010.1839623333231282No Hit
GGAATACCGGGTGCTGTAGGCTT142700.18356356174540517No Hit
TGAGGTAGTAGTTTGTACAGTT142170.18288179098349164No Hit
TGTAAACATCCTACACTCTCAGCT133290.17145891482161918No Hit
TTAATGCTAATCGTGATAGGGGTT132220.1700825096985107No Hit
CAACACCAGTCGATGGGCTGT132170.17001819170210375No Hit
AACGGAATCCCAAAAGCAGCTG125970.16204276014764324No Hit
CTACGCCTGTCTGAGCGTCGCT120180.15459473616371966No Hit
CCCCCACTGCTAAATTTGACTGGCTT114030.14668362260566611No Hit
CCCCCCACTGCTAAATTTGACTGT112660.14492130950411597No Hit
TGATGTAGTAGGTTGTATAGTT110910.14267017962987308No Hit
CAAAGAATTCTCCTTTTGGGCT109920.14139668330101568No Hit
AGCTCGGTCTGAGGCCCCTCAGT101360.13038544231614765No Hit
TATTGCACTTGTCCCGGCCTGTT101350.13037257871686628No Hit
TTCAAGTAATCCAGGATAGGC99950.12857167481747195No Hit
ACCGGGTGCTGTAGGCTTT95180.12243573796024992No Hit
CCCCCCACTGCTAAATTTGACTGTA94090.12103360563857865No Hit
ACCGGGTGCTGTAGGCTTA91980.11831938619020581No Hit
TGTCAGTTTGTCAAATACCCCA89890.11563089394039575No Hit
CCCCCCACTGCTAAATTTGACTGA89710.11539934915333076No Hit
TGAGGTAGTAGTTTGTGCTGTT88910.11437026121081974No Hit
TTCACAGTGGCTAAGTTCCGC85970.11058836302209168No Hit
CACTAGATTGTGAGCTCCTGGA84970.10930200309395291No Hit
TTCAAGTAATCCAGGATAGGCTT83120.10692223722689614No Hit
GGGAATACCGGGTGCTGTAGGCTT82680.10635623885851507No Hit
TGTAAACATCCCCGACTGGAAGC81870.10531428731672267No Hit
TAGCTTATCAGACTGATGTTGACA81440.10476115254762297No Hit
TCGTTTCCCGGCCAATGCACCA81380.10468397095193464No Hit
GCCTGTCTGAGCGTCGCA81020.10422088137780469No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)