FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8273459
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG181669021.958046809683836No Hit
CCCCCACTGCTAAATTTGACTG5374346.495880380866092No Hit
CAACGGAATCCCAAAAGCAGCTG4565965.518804166431477No Hit
AAGCTGCCAGTTGAAGAACTGT2220382.6837384460356906No Hit
TAGCAGCACGTAAATATTGGCG2141362.5882282126496308No Hit
TTCAAGTAATCCAGGATAGGCT1862162.250763556089418No Hit
AACATTCAACGCTGTCGGTGAGT1526931.8455763182001625No Hit
TAGCTTATCAGACTGATGTTGAC1129001.3646045747008597No Hit
AACATTCAACGCTGTCGGTGAG952321.1510542325767252No Hit
GCCTGTCTGAGCGTCGCT811090.9803517488876177No Hit
TAGCTTATCAGACTGATGTTGACT740690.8952603741675641No Hit
TGTAAACATCCTTGACTGGAAGCT696020.8412684464865299No Hit
TATTGCACTTGTCCCGGCCTGTAT574630.6945462593094376No Hit
TATTGCACTTGTCCCGGCCTGTAA571100.6902796037304348No Hit
TAGCTTATCAGACTGATGTTGA559710.6765126895534262No Hit
TATTGCACTTGTCCCGGCCTGT540860.6537289904984119No Hit
AGCAGCATTGTACAGGGCTATGA505520.611014087336385No Hit
TGAGGTAGTAGGTTGTATAGTT492760.5955912756683752No Hit
GACCCGCGGGCGCTCTCCAGTCCT484160.5851965906883687No Hit
TATTGCACTTGTCCCGGCCTGTA400230.4837517173893048No Hit
TTAATGCTAATCGTGATAGGGGTT360650.43591199279527465No Hit
TGTAAACATCCCCGACTGGAAGCT357240.43179037933227205No Hit
CATAAAGTAGAAAGCACTACT351350.42467122880526753No Hit
ACTGGACTTGGAGTCAGAAGGC332670.40209300608125326No Hit
TTCAAGTAATTCAGGATAGGTT318670.3851714258812427No Hit
TTCTCACTACTGCACTTGACTAG310250.37499430407523626No Hit
TGAGGTAGTAGATTGTATAGTT302290.36537317704723016No Hit
GGAATACCGGGTGCTGTAGGCTTT289410.3498053232632204No Hit
CAACGGAATCCCAAAAGCAGCTGT277480.33538571956421126No Hit
ACCCCCCACTGCTAAATTTGACTG271070.32763805320120637No Hit
TGTAAACATCCTACACTCTCAGCT252020.3046126172861919No Hit
CCCCCCACTGCTAAATTTGACTGG245400.29661112722018684No Hit
TCGTACGACTCTTAGCGG243460.2942662796781854No Hit
GGAATACCGGGTGCTGTAGGCTT233870.28267499724117806No Hit
CGCCTGTCTGAGCGTCGCT229010.2768007915431744No Hit
CTTCTCACTACTGCACTTGACTAG219740.26559628808216734No Hit
CCCATAAAGTAGAAAGCACTAC216560.2617526720081649No Hit
CATTGCACTTGTCTCGGTCTGA187980.22720847471414313No Hit
AACCGGGCGGAAACACCA183220.2214551374461395No Hit
CAAAGAATTCTCCTTTTGGGCT171510.2073014442931306No Hit
CCCCCCACTGCTAAATTTGACTGT162810.19678589088312398No Hit
CCCCCACTGCTAAATTTGACTGGCTT159040.1922291510721211No Hit
CCCCCCACTGCTAAATTTGACTGA155100.18746693493011812No Hit
CCCCCCACTGCTAAATTTGACTGTA154880.18720102438411793No Hit
TAGCTTATCAGACTGATGTTGACA151900.18359914517011566No Hit
CTCCCACTGCTTCACTTGACTAG151890.18358705832711564No Hit
ACCGGGTGCTGTAGGCTT146470.17703598942111154No Hit
CCCCCACAACCGCGCTTGACTAG145810.17623825778311103No Hit
AGCTCGGTCTGAGGCCCCTCAGT140820.17020692312610722No Hit
CCCATAAAGTAGAAAGCACT140730.17009814153910716No Hit
GGGCTACGCCTGTCTGAGCGTCGCT140300.16957840729010684No Hit
TGTCAGTTTGTCAAATACCCCA139700.16885319671010637No Hit
AACATTCAACGCTGTCGGTGAGTT136860.16542053329810422No Hit
CACCCGGCTGTGTGCACATGTGC134470.1625317778211024No Hit
GACCCGCGGGCGCTCTCCAGTCCTT131190.1585672933170999No Hit
CAACGGAATCCCAAAAGCAGCT127320.15388968507609696No Hit
CCCCCCACTGCTAAATTTGACTGGCT126830.15329742976909658No Hit
TGAGGTAGTAGTTTGTACAGTT125280.1514239691040954No Hit
TTCACAGTGGCTAAGTTCCG118540.14327743692209027No Hit
TAGCAGCACGTAAATATTGGC117400.1418995368200894No Hit
CTACGCCTGTCTGAGCGTCGCT115190.1392283445170877No Hit
GTGCGGTAACGCGACCGATCCCGGA114830.13879321816908743No Hit
CGCGACCTCAGATCAGACGTGGCG109500.1323509308500834No Hit
TGTAAACATCCCCGACTGGAAGC108310.1309125965330825No Hit
TGTAAACATCCTACACTCAGCT105150.12709315414508007No Hit
CTCCCACTGCTTCACTTGACTA104700.12654924621007974No Hit
TGAGGTAGTAGTTTGTGCTGTT104340.12611411986207943No Hit
CCCCCCACTGCTAAATTTGAC103360.12492960924807871No Hit
TATTGCACTTGTCCCGGCCTGTT103150.12467578554507855No Hit
AACGGAATCCCAAAAGCAGCTG102690.1241197907670782No Hit
TACGCCTGTCTGAGCGTCGCT102670.12409561708107819No Hit
GCGTTCGCGCTTTCCCCTG100230.12114642738907631No Hit
TTCAAGTAATCCAGGATAGGCTT98980.11963557201407538No Hit
TGTCAGTTTGTCAAATACCCCAA97870.11829393244107453No Hit
GGGAATACCGGGTGCTGTAGGCTT95810.11580404278307295No Hit
GGGAATACCGGGTGCTGTAGGCTTT91430.11051000554906962No Hit
AGCAGCATTGTACAGGGCTATG91030.11002653182906931No Hit
AGCAGCATTGTACAGGGCTATGT89670.10838272118106829No Hit
CAACACCAGTCGATGGGCTGT86780.10488962355406609No Hit
ACCGGGTGCTGTAGGCTTT85960.10389850242806546No Hit
CCCCCCACAACCGCGCTTGACTAG85510.10335459449306511No Hit
CAAAGAATTCTCCTTTTGGGCTT85220.1030040760460649No Hit
CAAAGTGCTGTTCGTGCAGGTAG84250.10183165227506416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)