FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5392822
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG126604523.476484111658053No Hit
CAACGGAATCCCAAAAGCAGCTG4174077.740047789450495No Hit
CCCCCACTGCTAAATTTGACTG4108507.618460242151512No Hit
TAGCAGCACGTAAATATTGGCG1371452.543102664986903No Hit
TTCAAGTAATCCAGGATAGGCT1314462.4374251551414083No Hit
AACATTCAACGCTGTCGGTGAGT930131.7247556103279507No Hit
AACATTCAACGCTGTCGGTGAG817241.5154217958612393No Hit
AAGCTGCCAGTTGAAGAACTGT772051.4316252233060909No Hit
TAGCTTATCAGACTGATGTTGAC617231.144539908789869No Hit
TAGCTTATCAGACTGATGTTGACT518730.9618897119170631No Hit
TATTGCACTTGTCCCGGCCTGTAA510380.9464061673090638No Hit
GCCTGTCTGAGCGTCGCT493840.9157357687681885No Hit
TATTGCACTTGTCCCGGCCTGTAT464750.8617936953973263No Hit
TGTAAACATCCTTGACTGGAAGCT386990.7176020272873831No Hit
TGAGGTAGTAGGTTGTATAGTT367180.6808680130736746No Hit
TAGCTTATCAGACTGATGTTGA346380.6422982253076405No Hit
GACCCGCGGGCGCTCTCCAGTCCT298380.5532910227706385No Hit
CCCCCACTGCTAAATTTGACTGGCTT287170.5325041323448094No Hit
TATTGCACTTGTCCCGGCCTGT275070.5100669000386069No Hit
TATTGCACTTGTCCCGGCCTGTA265060.49150518967620294No Hit
CAACGGAATCCCAAAAGCAGCT256160.47500177087246714No Hit
AGCAGCATTGTACAGGGCTATGA251810.46693549314255134No Hit
ACCCCCCACTGCTAAATTTGACTG238880.4429591779591464No Hit
TGTAAACATCCCCGACTGGAAGCT233050.43214851148433975No Hit
TGAGGTAGTAGATTGTATAGTT229520.42560277346443104No Hit
CAACGGAATCCCAAAAGCAGCTGT215520.39964233939113886No Hit
TTCAAGTAATTCAGGATAGGTT206010.3820077873884953No Hit
TTCTCACTACTGCACTTGACTAG203850.3780024632743302No Hit
ACTGGACTTGGAGTCAGAAGGC197720.3666355017836673No Hit
CATAAAGTAGAAAGCACTACT179040.3319968654630173No Hit
GGAATACCGGGTGCTGTAGGCTTT178510.3310140776016713No Hit
CCCATAAAGTAGAAAGCACTAC153440.2845263574432829No Hit
CCCCCCACTGCTAAATTTGACTGG150480.27903757995350115No Hit
GGAATACCGGGTGCTGTAGGCTT145200.26924678767443094No Hit
CTTCTCACTACTGCACTTGACTAG144950.26878320849455073No Hit
CACCCGGCTGTGTGCACATGTGC139870.25936327955938465No Hit
CAAAGAATTCTCCTTTTGGGCT130560.24209959090064534No Hit
CCCCCCACTGCTAAATTTGACTGGCT121240.2248173590747108No Hit
TAGCAGCACGTAAATATTGGC117130.21719611735747998No Hit
TAGCTTATCAGACTGATGTTGACA116290.21563849131308246No Hit
CATTGCACTTGTCTCGGTCTGA110400.20471656583510453No Hit
CTCCCACTGCTTCACTTGACTAG108310.20084104389130591No Hit
CCCCCCACTGCTAAATTTGACTGTA98430.18252039470243964No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC93190.17280377509215028No Hit
AACGGAATCCCAAAAGCAGCTG92890.172247480076294No Hit
CTACGGGGATGATTTTACGAACTGAA92650.171802444063609No Hit
CCCCCCACTGCTAAATTTGACTGT92100.17078256986787252No Hit
TGTAAACATCCTACACTCTCAGCT91660.16996667051128334No Hit
TCGTTTCCCGGCCAATGCACCA91510.1696885230033552No Hit
TGAGGTAGTAGTTTGTACAGTT87920.16303152598027526No Hit
CCCCCCACTGCTAAATTTGACTGA84960.15754274849049346No Hit
TGTAAACATCCCCGACTGGAAGC76100.14111350235553852No Hit
CTCCCACTGCTTCACTTGACTAGCCT76080.14107641602114812No Hit
GACCCGCGGGCGCTCTCCAGTCCTT75770.14050157783809664No Hit
GTGCGGTAACGCGACCGATCCCGGA73770.1367929443990549No Hit
CCCATAAAGTAGAAAGCACT70890.13145251224683477No Hit
CACTAGATTGTGAGCTCCTGGAA69310.1285226918299918No Hit
CCCCCACAACCGCGCTTGACTAG68830.1276326198046218No Hit
CAAAGAATTCTCCTTTTGGGCTT67760.12564850091473445No Hit
CGCGACCTCAGATCAGACGTGGCG66780.12383127052960398No Hit
TGTCAGTTTGTCAAATACCCCA65750.12192132430849748No Hit
CACTAGATTGTGAGCTCCTGGA63990.11865772688214075No Hit
AGCTCGGTCTGAGGCCCCTCAGT63990.11865772688214075No Hit
TATTGCACTTGTCCCGGCCTGTAG62610.11609876980920193No Hit
AACATTCAACGCTGTCGGTGAGTT62600.11608022664200673No Hit
TATTGCACTTGTCCCGGCCTGTT62540.11596896763883548No Hit
GTCAGGATGGCCGAGCGGTCTAAGG62070.11509743878066066No Hit
TGAGGTAGTAGTTTGTGCTGTT61870.11472657543675649No Hit
NCCCCCACTGCTAAATTTGACTG61490.11402193508333856No Hit
GGGAATACCGGGTGCTGTAGGCTT61290.1136510717394344No Hit
AACCGGGCGGAAACACCA60840.11281662921564999No Hit
CAACGGAATCCCAAAAGCAGCTGA60660.11248285220613623No Hit
CCCCCCACTGCTAAATTTGAC60230.11168549601674226No Hit
TTCAAGTAATCCAGGATAGGCTT59950.1111662873352764No Hit
ACTGGACTTGGAGTCAGAAGGCT59080.10955303178929324No Hit
GGGAATACCGGGTGCTGTAGGCTTT58560.1085887870951424No Hit
AACACCCTGATTGCTCCTATCTGATT58050.10764308556818676No Hit
ACTGGACTTGGAGTCAGAAGGCA57230.10612254585817962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)