FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6431913
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5393658.385763302457605No Hit
CCCCCCACTGCTAAATTTGACTG3642695.6634627987039No Hit
TAGCAGCACGTAAATATTGGCG2065993.212092576500957No Hit
AACATTCAACGCTGTCGGTGAGT1937773.012742865147585No Hit
AAGCTGCCAGTTGAAGAACTGT1889592.9378351355187795No Hit
TTCAAGTAATCCAGGATAGGCT1876372.917281374919095No Hit
CCCCCACTGCTAAATTTGACTG1291522.0079873592817563No Hit
AACATTCAACGCTGTCGGTGAG1158521.8012059553666229No Hit
TATTGCACTTGTCCCGGCCTGTAA1089001.6931199162675243No Hit
TATTGCACTTGTCCCGGCCTGTAT873251.3576831651796284No Hit
TGAGGTAGTAGGTTGTATAGTT771201.1990211932282044No Hit
TAGCTTATCAGACTGATGTTGAC703031.0930340631162143No Hit
TATTGCACTTGTCCCGGCCTGT702951.0929096833243857No Hit
TAGCTTATCAGACTGATGTTGACT633650.9851656886528161No Hit
TAGCTTATCAGACTGATGTTGA528090.8210465533349097No Hit
TGTAAACATCCTTGACTGGAAGCT466860.7258493701640554No Hit
CATAAAGTAGAAAGCACTACT461770.7179357059089574No Hit
TTCAAGTAATTCAGGATAGGTT450960.7011288865381108No Hit
TGAGGTAGTAGATTGTATAGTT445360.6924223011101052No Hit
TATTGCACTTGTCCCGGCCTGTA430940.6700028436329907No Hit
AGCAGCATTGTACAGGGCTATGA382670.5949551867383779No Hit
CAACGGAATCCCAAAAGCAGCTGT323160.5024321690918394No Hit
TGTAAACATCCCCGACTGGAAGCT300730.46755918495788107No Hit
ACTGGACTTGGAGTCAGAAGGC293030.45558762999437336No Hit
GGAATACCGGGTGCTGTAGGCTTT276880.43047845951896424No Hit
CACCCGGCTGTGTGCACATGTGC276270.4295300636062708No Hit
GCCTGTCTGAGCGTCGCT256500.39879270755061524No Hit
TGATGTAGTAGGTTGTATAGTT254020.394936934003927No Hit
ACCGGGTGCTGTAGGCTT242900.37764814293974436No Hit
CATTGCACTTGTCTCGGTCTGA232780.3619140992734199No Hit
ACCGGGTGCTGTAGGCTTA224130.34846553428194693No Hit
CCCATAAAGTAGAAAGCACTAC222030.34520056474644484No Hit
CGCCTGTCTGAGCGTCGCT219480.34123595888190655No Hit
TTCTCACTACTGCACTTGACTAG215260.33467492486294514No Hit
GGAATACCGGGTGCTGTAGGCTT214220.33305798756917265No Hit
TGAGGTAGTAGTTTGTACAGTT193080.3001906275784514No Hit
ACCGGGTGCTGTAGGCTTT190870.2967546358291849No Hit
CCCATAAAGTAGAAAGCACT169760.26393391826039936No Hit
CTTCTCACTACTGCACTTGACTAG161230.25067192295666935No Hit
CGCGACCTCAGATCAGACGTGG160420.24941257756440424No Hit
TGAATACCGGGTGCTGTAGGCTTT158510.24644301003449517No Hit
CTCCCACTGCTTCACTTGACTAG156330.24305366070716444No Hit
AACCGGGCGGAAACACCA151800.236010654994867No Hit
TGATGTAGTAGATTGTATAGTT147790.22977611792945582No Hit
CCCCCACTGCTAAATTTGACTGGCTT147140.22876553212084805No Hit
ACCCCCCACTGCTAAATTTGACTG140000.21766463570014083No Hit
CAACGGAATCCCAAAAGCAGCT139190.21640529030787573No Hit
CAACACCAGTCGATGGGCTGT137340.21352900762183816No Hit
TCGTACGACTCTTAGCGG133240.20715454329061975No Hit
TAGCAGCACGTAAATATTGGC128370.19958292346305057No Hit
CGCGACCTCAGATCAGACGTGGCG126960.19739072963207058No Hit
TCCTGTCTGAGCGTCGCT125360.19490313379549754No Hit
CCCCCCACTGCTAAATTTGACTGG123360.19179363899978125No Hit
TGAATACCGGGTGCTGTAGGCTT121460.18883961894385076No Hit
AACATTCAACGCTGTCGGTGAGTT120770.18776684323932863No Hit
TGTAAACATCCTACACTCTCAGCT120430.18723822912405685No Hit
AGCTCGGTCTGAGGCCCCTCAGT116080.18047507794337392No Hit
TATTGCACTTGTCCCGGCCTGTAG114030.1772878457777647No Hit
AACGGAATCCCAAAAGCAGCTG112610.17508010447280614No Hit
TGTCAGTTTGTCAAATACCCCAA109230.16982505826804561No Hit
CTACGCCTGTCTGAGCGTCGCT107410.16699541800394377No Hit
TATTGCACTTGTCCCGGCCTGTT104790.16292197982155543No Hit
ATCCCGGACGAGCCCCCA104530.16251774549811232No Hit
TGAGGTAGTAGTTTGTGCTGTT101540.15786905077851643No Hit
ACTTGACTTGGAGTCAGAAGGC100880.15684291749593007No Hit
CAAAGAATTCTCCTTTTGGGCT99210.15424648934150695No Hit
CACTAGATTGTGAGCTCCTGGA97190.1511058995978335No Hit
TTCAAGTAATCCAGGATAGGCTT96410.14989319662750414No Hit
CAAAGAATTCTCCTTTTGGGCTT92690.1441095363074718No Hit
TGAGGTAGTAGGTTGTGTGGTT91700.14257033638359226No Hit
AGTTCTACAGTCCGACGATC90470.14065799708422672No Hit
CCCCCCACTGCTAAATTTGACTGGCT89510.13916543958228292No Hit
TTCACAGTGGCTAAGTTCCGC89360.1389322274726042No Hit
TGTAAACATCCCCGACTGGAAGC89340.13890113252464703No Hit
CAGAGTTCTACAGTCCGACGATC88200.13712872049108873Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTCAAGTAATCCAGGATAGGC85150.1323867409276214No Hit
TGTCAGTTTGTCAAATACCCCA84950.13207579144804976No Hit
TTCACAGTGGCTAAGTTCTGC83910.13045885415427727No Hit
CACTAGATTGTGAGCTCCTGGAA83680.13010126225276988No Hit
TAGCTTATCAGACTGATGTTGACA83110.12921505623599075No Hit
TTATAAAGCAATGAGACTGATT81920.12736490683253957No Hit
TATTGCACTTGTCCCGGCCTGTAAT81680.12699176745705362No Hit
ATCCCACCGCTGCCACCA80670.12542147258521688No Hit
TGAGGTAGTAGGTTGTATAGT73750.1146626205920385No Hit
CAAAGTGCTGTTCGTGCAGGT72990.11348101256966629No Hit
ACCTGGTGCTGTAGGCTT72520.11275028129267296No Hit
CGCGACCTCAGATCAGACGTGGC72300.11240823686514416No Hit
ACTGGACTTGGAGTCAGAAGGCA72250.11233049949525126No Hit
CAACGGAATCCCAAAAGCAGCTGA72030.11198845506772245No Hit
CAAAGTGCTGTTCGTGCAGGTAG70490.10959414407502091No Hit
TCGTTTCCCGGCCAATGCACCA70250.10922100469953495No Hit
ACCACAGGGTAGAACCACGGAC70230.10918990975157779No Hit
AGCAGCATTGTACAGGGCTATGT69650.10828815626082007No Hit
ACGCCTGTCTGAGCGTCGCT69560.10814822899501284No Hit
TATTGCACTTGTCCCGGCCTGTAAA68220.10606486748188293No Hit
TTCACAGTGGCTAAGTTCCG67530.1049920917773608No Hit
TACGCCTGTCTGAGCGTCGCT66740.10376384133305287No Hit
ACCTGGTGCTGTAGGCTTA66480.10335960700960975No Hit
AGCAGCATTGTACAGGGCTATG66040.10267551815455216No Hit
ACTGGACTTGGAGTCAGAAGGCT64690.10057660916744364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)