FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5106444
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG3996067.825523984988379No Hit
CAACGGAATCCCAAAAGCAGCTG3714747.274612235050458No Hit
TATTGCACTTGTCCCGGCCTGTAA1548743.0329129233572325No Hit
TATTGCACTTGTCCCGGCCTGTAT1477922.8942254140063026No Hit
TAGCAGCACGTAAATATTGGCG1094942.144231876429077No Hit
CCCCCACTGCTAAATTTGACTG1041742.0400497880716992No Hit
AACATTCAACGCTGTCGGTGAGT1033552.0240112297324715No Hit
TTCAAGTAATCCAGGATAGGCT953711.8676597647991442No Hit
AAGCTGCCAGTTGAAGAACTGT886561.736159252896928No Hit
AACATTCAACGCTGTCGGTGAG747251.4633470963355322No Hit
TAGCTTATCAGACTGATGTTGACT693821.358714596693903No Hit
TAGCTTATCAGACTGATGTTGAC618001.2102355376853247No Hit
TATTGCACTTGTCCCGGCCTGT541821.0610514871013959No Hit
TATTGCACTTGTCCCGGCCTGTA516531.011525828933011No Hit
GGAATACCGGGTGCTGTAGGCTTT503590.9861852984190173No Hit
GCCTGTCTGAGCGTCGCT466080.9127290928873399No Hit
TGAGGTAGTAGGTTGTATAGTT461090.902957126329007No Hit
AGCAGCATTGTACAGGGCTATGA391640.7669525015842728No Hit
TGTAAACATCCTTGACTGGAAGCT384010.7520105968067015No Hit
GGAATACCGGGTGCTGTAGGCTT325690.6378019615998922No Hit
TGTAAACATCCCCGACTGGAAGCT325540.6375082151101628No Hit
TAGCTTATCAGACTGATGTTGA315400.6176509524044521No Hit
TGAGGTAGTAGATTGTATAGTT301590.5906066922500276No Hit
TTAATGCTAATCGTGATAGGGGTT295040.5777797621985084No Hit
ACTGGACTTGGAGTCAGAAGGC281330.5509313330372368No Hit
TTCAAGTAATTCAGGATAGGTT273420.5354411014788374No Hit
CAACGGAATCCCAAAAGCAGCTGT225910.4424017966318636No Hit
CCCCCACTGCTAAATTTGACTGGCTT218220.42734239325840057No Hit
TATTGCACTTGTCCCGGCCTGTAG205850.4031180994053788No Hit
TGAATACCGGGTGCTGTAGGCTTT195900.38363291558665874No Hit
CACCCGGCTGTGTGCACATGTGC193590.37910921964482525No Hit
TGATGTAGTAGGTTGTATAGTT172830.33845470546626966No Hit
CACTAGATTGTGAGCTCCTGGAA171930.336692226527893No Hit
ACCCCCCACTGCTAAATTTGACTG170930.33473391659636337No Hit
TCCTGTCTGAGCGTCGCT164210.32157407385648407No Hit
TATTGCACTTGTCCCGGCCTGTAAT152710.2990535096438931No Hit
CACTAGATTGTGAGCTCCTGGA145550.2850320105341408No Hit
CCCATAAAGTAGAAAGCACTAC144980.2839157738731689No Hit
CATAAAGTAGAAAGCACTACT144120.28223162733205337No Hit
CATTGCACTTGTCTCGGTCTGA140860.27584753695526676No Hit
TATTGCACTTGTCCCGGCCTGTAAA138230.27069718183534375No Hit
CAAAGAATTCTCCTTTTGGGCT133340.26112104627016375No Hit
TATTGCACTTGTCCCGGCCTGTTT128800.2522303191810191No Hit
CTCCCACTGCTTCACTTGACTAG126220.2471778795576726No Hit
TGAATACCGGGTGCTGTAGGCTT125100.24498457243435942No Hit
TATTGCACTTGTCCCGGCCTGTT118300.23166806489995778No Hit
ACTTGACTTGGAGTCAGAAGGC118050.23117848741707533No Hit
AGCTCGGTCTGAGGCCCCTCAGT117860.23080640853008474No Hit
TGATGTAGTAGATTGTATAGTT115580.22634146188619714No Hit
TTCTCACTACTGCACTTGACTAG115450.22608688159509827No Hit
TGAGAACTGAATTCCATAGGCTGT113970.2231885828964344No Hit
ACCGGGTGCTGTAGGCTTA113170.22162193495121066No Hit
CCCCCCACTGCTAAATTTGACTGGCT109320.21408244171482152No Hit
CTTCTCACTACTGCACTTGACTAG109230.21390619382098383No Hit
TAGCTTATCAGACTGATGTTGACA108730.212927038855219No Hit
ACTGGACTTGGAGTCAGAAGGCA105410.20642544988254055No Hit
TGAGGTAGTAGTTTGTACAGTT98830.19353977053307547No Hit
GGAATACCGGGTGCTGTAGGCTTA95690.18739067734807235No Hit
CCCCCCACTGCTAAATTTGACTGG94360.18478612513913792No Hit
TGTAAACATCCTACACTCTCAGCT93830.1837482208754272No Hit
CAAAGAATTCTCCTTTTGGGCTT93750.18359155608090483No Hit
CGCGACCTCAGATCAGACGTGGCG93220.1825536518171941No Hit
CAACGGAATCCCAAAAGCAGCT88590.17348667683421182No Hit
AGCTACATCTGGCTACTGGGTCTC81250.15911268193678418No Hit
TGTAAACATCCCCGACTGGAAGC80460.15756561709087574No Hit
CCCCCCACTGCTAAATTTGACTGT78280.15329650144014112No Hit
TGAGAACTGAATTCCATGGGTT76450.14971279426544185No Hit
AACATTCAACGCTGTCGGTGAGTT74980.14683407866609327No Hit
TAGCAGCACGTAAATATTGGC74250.14540451241607663No Hit
AACCGGGCGGAAACACCA73010.14297620810097986No Hit
ACCGGGTGCTGTAGGCTT72280.14154664185096322No Hit
CCCATAAAGTAGAAAGCACT71130.1392945854297041No Hit
ATCCCGGACGAGCCCCCA68160.13347840493306104No Hit
CGCGACCTCAGATCAGACGTGG66840.1308934358234419No Hit
ACCGGGTGCTGTAGGCTTT65640.12854346390560634No Hit
AGCAGCATTGTACAGGGCTATGT65620.12850429770697572No Hit
TGAGGTAGTAGTTTGTGCTGTT65450.1281713850186157No Hit
TATTGCACTTGTCCCGGCCTGTATT62470.12233562142265735No Hit
CTCCCACTGCTTCACTTGACTAGCCT62470.12233562142265735No Hit
TTCAAGTAATCCAGGATAGGCTT62320.12204187493292788No Hit
GGAATACCGGGTGCTGTAGGCT61970.12135646645689252No Hit
TATTGCACTTGTCCCGGCCTGTTA61480.12039689459044298No Hit
CAAAGTGCTGTTCGTGCAGGTAG61450.12033814529249709No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC58380.11432613380270104No Hit
AACGGAATCCCAAAAGCAGCTG57300.11221115907664903No Hit
TATTGCACTTGTCCCGGCCTGA56560.1107620097273171No Hit
TTAATGCTAATCGTGATAGGGGTTT56540.1107228435286865No Hit
TCGTACGACTCTTAGCGG56310.11027243224443468No Hit
TCGTTTCCCGGGCGGCGCACCA55340.10837287161085092No Hit
GGGAATACCGGGTGCTGTAGGCTT54130.10600331659370005No Hit
ATGCACCTGGGCAAGGATTCTGA52800.10339876438476561No Hit
ACTGGACTTGGAGTCAGAAGGCT52180.10218461222721723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)