FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7504867
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6488838.646162550249057No Hit
CCCCCCACTGCTAAATTTGACTG4930716.570016497294357No Hit
AACATTCAACGCTGTCGGTGAGT1944822.591411679913848No Hit
TATTGCACTTGTCCCGGCCTGTAA1931702.5739296912257075No Hit
CCCCCACTGCTAAATTTGACTG1892822.5221233101132903No Hit
TTCAAGTAATCCAGGATAGGCT1551392.0671785389401305No Hit
TAGCAGCACGTAAATATTGGCG1509462.0113081284451813No Hit
TATTGCACTTGTCCCGGCCTGTAT1436201.9136914751453955No Hit
AAGCTGCCAGTTGAAGAACTGT1415771.8864691406256768No Hit
AACATTCAACGCTGTCGGTGAG1408831.8772218081946024No Hit
TAGCTTATCAGACTGATGTTGACT1177661.5691950303716242No Hit
TAGCTTATCAGACTGATGTTGAC1135681.5132579964441741No Hit
TGAGGTAGTAGGTTGTATAGTT890921.1871229696675505No Hit
GCCTGTCTGAGCGTCGCT808551.0773675269661676No Hit
TATTGCACTTGTCCCGGCCTGT769051.0247350152907442No Hit
TAGCTTATCAGACTGATGTTGA653100.8702352753220011No Hit
TCGTACGACTCTTAGCGG622240.8291152927826703No Hit
TATTGCACTTGTCCCGGCCTGTA613910.8180158289280809No Hit
TCCTGTCTGAGCGTCGCT540150.7197329413032902No Hit
AGCAGCATTGTACAGGGCTATGA456710.6085517571463959No Hit
TGTAAACATCCTTGACTGGAAGCT444070.5917093534102603No Hit
TGAGGTAGTAGATTGTATAGTT439590.5857398938582122No Hit
CACCCGGCTGTGTGCACATGTGC397140.5291765996652572No Hit
ACTGGACTTGGAGTCAGAAGGC373620.49783693701700504No Hit
TGTAAACATCCCCGACTGGAAGCT357700.47662403610883447No Hit
TTCAAGTAATTCAGGATAGGTT352710.4699750175452809No Hit
CAACGGAATCCCAAAAGCAGCTGT351890.46888239325227216No Hit
CATTGCACTTGTCTCGGTCTGA326690.435304183272002No Hit
TGATGTAGTAGGTTGTATAGTT320950.4276558132209405No Hit
CATAAAGTAGAAAGCACTACT308040.4104536429493021No Hit
CAACGGAATCCCAAAAGCAGCT306590.4085215634067866No Hit
TTAATGCTAATCGTGATAGGGGTT274770.36612241096344544No Hit
CCCCCACTGCTAAATTTGACTGGCTT259520.34580226405078196No Hit
ACCGGGTGCTGTAGGCTT229750.30613467233996283No Hit
CGCGACCTCAGATCAGACGTGG216060.2878931765213161No Hit
TATTGCACTTGTCCCGGCCTGTAG212060.2825633019212732No Hit
ACCGGGTGCTGTAGGCTTA211260.2814973270012647No Hit
CCCATAAAGTAGAAAGCACTAC208990.27847262316574034No Hit
TATTGCACTTGTCCCGGCCTGTAAT187620.24999776811501123No Hit
TGAGAACTGAATTCCATAGGCTGT186030.2478791429614942No Hit
ACCCCCCACTGCTAAATTTGACTG185040.24655999899798356No Hit
CACTAGATTGTGAGCTCCTGGA182690.24342869767045838No Hit
CACTAGATTGTGAGCTCCTGGAA179570.23927139548242496No Hit
ACCGGGTGCTGTAGGCTTT175730.23415471586638378No Hit
CAACACCAGTCGATGGGCTGT171160.2280653341358348No Hit
TAGCTTATCAGACTGATGTTGACA164920.21975072975976792No Hit
CCCATAAAGTAGAAAGCACT164490.21917776824026328No Hit
TATTGCACTTGTCCCGGCCTGTAAA162140.2160464669127381No Hit
TGATGTAGTAGATTGTATAGTT159260.2122089572007072No Hit
ACTTGACTTGGAGTCAGAAGGC145570.19396746138206047No Hit
CGCGACCTCAGATCAGACGTGGCG145300.19360769484655757No Hit
TGAGGTAGTAGTTTGTACAGTT138730.18485337581598715No Hit
TCGTTTCCCGGCCAATGCACCA137230.18285467284097107No Hit
AACATTCAACGCTGTCGGTGAGTT129790.1729411060848913No Hit
TATTGCACTTGTCCCGGCCTGTT129520.17258133954938842No Hit
CAAAGAATTCTCCTTTTGGGCT127460.16983645413036633No Hit
AACCGGGCGGAAACACCA120410.16044255014779077No Hit
TAGCAGCACGTAAATATTGGC120370.16038925140179033No Hit
GGAATACCGGGTGCTGTAGGCTTT120090.16001616017978734No Hit
AACGGAATCCCAAAAGCAGCTG120060.159976186120287No Hit
TGAGAACTGAATTCCATGGGTT117680.1568049107332615No Hit
CAACGGAATCCCAAAAGCAGCTGA116720.1555257408292512No Hit
CTCCCACTGCTTCACTTGACTAG116200.15483285713124564No Hit
TTCTCACTACTGCACTTGACTAG114550.15263428385872796No Hit
TGTAAACATCCTACACTCTCAGCT111520.14859690384919547No Hit
AGCTCGGTCTGAGGCCCCTCAGT107350.14304050957865078No Hit
TTCAAGTAATCCAGGATAGGCTT101230.13488580144058515No Hit
CTTCTCACTACTGCACTTGACTAG100950.13451271021858216No Hit
CAAAGAATTCTCCTTTTGGGCTT100790.13429951523458045No Hit
CGCGACCTCAGATCAGACGTGGC97230.1295559268405423No Hit
TGAGGTAGTAGTTTGTGCTGTT94740.12623807990201558No Hit
TTATAAAGCAATGAGACTGATT93300.12431932504600016No Hit
CGCCTGTCTGAGCGTCGCT91790.12230729738448395No Hit
CCCCCCACTGCTAAATTTGACTGG91290.12164106305947861No Hit
CCCCCCACTGCTAAATTTGACTGGCT90320.12034856846896821No Hit
GGAATACCGGGTGCTGTAGGCTT87400.11645776001093691No Hit
TATTGCACTTGTCCCGGCCTGTTT86490.11524521353942715No Hit
AGCAGCATTGTACAGGGCTATGT85320.11368622521891461No Hit
CACCCGTAGAACCGACCTTGCG85070.11335310805641192No Hit
CTCCCACTGCTTCACTTGACTAGCCT83260.11094133979989253No Hit
ACTGGACTTGGAGTCAGAAGGCA82380.10976876738788309No Hit
CTCCCCACTGCTAAATTTGACTG78250.10426567186333881No Hit
TGTAAACATCCCCGACTGGAAGC77700.10353281410583291No Hit
CAAAGTGCTGTTCGTGCAGGTAG77190.10285325509432745No Hit
TCAGTGCACTACAGAACTTTGT77110.10274665760232658No Hit
ACCACAGGGTAGAACCACGGAC77020.10262673542382564No Hit
TGCAAGTAATCCAGGATAGGCT76840.10238689106682371No Hit
TGAATACCGGGTGCTGTAGGCTTT76030.10130759146031501No Hit
TACCACCCTGAACGCGCCCGA75880.1011077211628134No Hit
TTCACAGTGGCTAAGTTCTGC75350.10040151277830774No Hit
ACCTGGTGCTGTAGGCTT75100.10006839561580505No Hit
ATGCACCTGGGCAAGGATTCTGA75060.10001509686980463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)