FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062006
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG1981234.877466946134496No Hit
CCCCCCACTGCTAAATTTGACTG1661464.090245066107731No Hit
AACATTCAACGCTGTCGGTGAGT1386443.413190428571499No Hit
AAGCTGCCAGTTGAAGAACTGT1115772.746844785556693No Hit
TTCAAGTAATCCAGGATAGGCT923312.2730394785236654No Hit
TAGCAGCACGTAAATATTGGCG823722.0278650499285327No Hit
CGCCTGTCTGAGCGTCGCT707861.7426365199854454No Hit
CCCCCACTGCTAAATTTGACTG678771.6710216577720465No Hit
AACATTCAACGCTGTCGGTGAG617731.5207510771771384No Hit
TATTGCACTTGTCCCGGCCTGTAA542451.3354239260109413No Hit
TATTGCACTTGTCCCGGCCTGT494331.2169602900635794No Hit
TAGCTTATCAGACTGATGTTGAC492751.2130705863063718No Hit
TATTGCACTTGTCCCGGCCTGTAT434791.0703824662001977No Hit
TGAGGTAGTAGGTTGTATAGTT393210.96801924960229No Hit
TAGCTTATCAGACTGATGTTGACT388630.95674403238203No Hit
CATAAAGTAGAAAGCACTACT335720.8264881932719941No Hit
TAGCTTATCAGACTGATGTTGA316970.7803287341279161No Hit
ACCGGGTGCTGTAGGCTT315250.776094373075766No Hit
TATTGCACTTGTCCCGGCCTGTA292220.719398248057733No Hit
AGCAGCATTGTACAGGGCTATGA261950.6448784172155334No Hit
TCGTACGACTCTTAGCGG256270.630895178392154No Hit
TGTAAACATCCTTGACTGGAAGCT238250.5865328608574187No Hit
TTAATGCTAATCGTGATAGGGGTT227060.5589848956402329No Hit
TGAGGTAGTAGATTGTATAGTT203230.500319300365386No Hit
ACCGGGTGCTGTAGGCTTT202800.4992607101023484No Hit
ACTGGACTTGGAGTCAGAAGGC201520.49610955769144605No Hit
ACCGGGTGCTGTAGGCTTA186290.4586157676773495No Hit
CCCATAAAGTAGAAAGCACTAC163630.40283052265309305No Hit
ATCCCGGACGAGCCCCCA159540.39276160596513154No Hit
TTCAAGTAATTCAGGATAGGTT150350.3701373163899807No Hit
ACGCCTGTCTGAGCGTCGCT148250.364967456965844No Hit
TGTAAACATCCCCGACTGGAAGCT148040.3644504710234303No Hit
TGATGTAGTAGGTTGTATAGTT144610.35600636729734025No Hit
CATTGCACTTGTCTCGGTCTGA144310.355267815951035No Hit
GCCTGTCTGAGCGTCGCT139350.3430571003587882No Hit
CTACGCCTGTCTGAGCGTCGCT138460.34086606469808267No Hit
TACGCCTGTCTGAGCGTCGCT134230.3304524907151786No Hit
AACATTCAACGCTGTCGGTGAGTT123340.30364307684429814No Hit
CAACGGAATCCCAAAAGCAGCTGT121880.30004879362561254No Hit
CCCATAAAGTAGAAAGCACT119490.29416500123338074No Hit
CAACACCAGTCGATGGGCTGT113990.2806248932177845No Hit
ACCTGGTGCTGTAGGCTT106100.26120099280995646No Hit
TCCTGTCTGAGCGTCGCT96620.23786277026671057No Hit
TGAGGTAGTAGTTTGTACAGTT96530.237641204862819No Hit
GCGTTCGCGCTTTCCCCTG95460.2350070383943303No Hit
CACCCGGCTGTGTGCACATGTGC90950.22390414982154136No Hit
CGCGACCTCAGATCAGACGTGG89670.220752997410639No Hit
ACTTGACTTGGAGTCAGAAGGC80250.19756248513665414No Hit
TGTAAACATCCTACACTCTCAGCT79680.19615923757867418No Hit
TAATGTGAATTGCAGGACA78820.19404205705259914No Hit
TTATAAAGCAATGAGACTGATT76400.1880844095257368No Hit
TTCACAGTGGCTAAGTTCCG74450.18328382577475266No Hit
TGATGTAGTAGATTGTATAGTT72490.17845862364555837No Hit
TATTGCACTTGTCCCGGCCTGTT70980.17474124853582196No Hit
AACCGGGCGGAAACACCA70250.17294410692647919No Hit
ACCTGGTGCTGTAGGCTTT69200.17035917721441082No Hit
CGCGACCTCAGATCAGACGTGGCG68170.16782348425876278No Hit
ACCACAGGGTAGAACCACGGAC67240.16553397508521653No Hit
TCGTTCGCGCTTTCCCCTG67050.1650662258992232No Hit
TTCTCACTACTGCACTTGACTAG64930.15984712971866608No Hit
ACCTGGTGCTGTAGGCTTA63060.15524349299336337No Hit
CTCCTGTCTGAGCGTCGCT62230.15320016760191887No Hit
CACTAGATTGTGAGCTCCTGGA60860.14982744978712487No Hit
TATTGCACTTGTCCCGGCCTGTAG60060.14785797953031088No Hit
TACCTGGTTGATCCTGCC59260.1458885092734969No Hit
AGCTCGGTCTGAGGCCCCTCAGT59000.14524843144003236No Hit
ATCCCACCGCTGCCACCA58660.1444114065808864No Hit
TGTCAGTTTGTCAAATACCCCAA58360.14367285523458115No Hit
TTCAAGTAATCCAGGATAGGCTT55720.13717360338709494No Hit
TGAGGTAGTAGTTTGTGCTGTT55580.13682894609215251No Hit
CTCCCACTGCTTCACTTGACTAG55350.1362627233933185No Hit
AGCTACATCTGGCTACTGGGTCTC53530.13178217855906663No Hit
AGCAGCATTGTACAGGGCTATG52790.1299604185715137No Hit
ACTGGACTTGGAGTCAGAAGGCA52570.12941881425088983No Hit
TTCAAGTAATCCAGGATAGGC51850.12764629101975725No Hit
GGAATACCGGGTGCTGTAGGCTTT51220.1260953331925162No Hit
ACTGGACTTGGAGTCAGAAGG50520.12437204671780396No Hit
CCCCCACTGCTAAATTTGACTGGCTT48960.12053157971701667No Hit
TGAGGTAGTAGGTTGTGTGGTT48920.12043310620417597No Hit
CCTCGACACAAGGGTTTGT48360.11905447702440616No Hit
CTTCTCACTACTGCACTTGACTAG47820.11772508460105673No Hit
TTCACAGTGGCTAAGTTCTG47500.11693729649833112No Hit
TAGCTTATCAGACTGATGTTGACA47370.11661725758159884No Hit
AGCAGCATTGTACAGGGCTATGT47050.11582946947887324No Hit
TGCAAGTAATCCAGGATAGGCT47000.11570637758782237No Hit
TACTCCTGTCTGAGCGTCGCT46740.11506629975435781No Hit
AACATTCAACGCTGTCGGTGA46570.11464778732478485No Hit
GGAATACCGGGTGCTGTAGGCTT45380.11171820031777402No Hit
CCCCCCACTGCTAAATTTGACTGG45120.11107812248430948No Hit
CAAAGTGCTGTTCGTGCAGGTAG45000.11078270194578738No Hit
ACCCCCCACTGCTAAATTTGACTG44430.1093794543878074No Hit
CAACGGAATCCCAAAAGCAGCT44420.10935483600959724No Hit
CGCGACCTCAGATCAGAC43320.10664681440647797No Hit
CACTAGATTGTGAGCTCCTGGAA43110.10612982846406431No Hit
TGAGGTAGTAGGTTGTATAGT42660.10502200144460642No Hit
AGCTACATTGTCTGCTGGGTTT41640.10251092686716858No Hit
AACGGAATCCCAAAAGCAGCTG41590.1023878349761177No Hit
TAGCAGCACGTAAATATTGGC41260.10157542849518193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)