FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14645385
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG9580486.54163751925948No Hit
CAACGGAATCCCAAAAGCAGCTG9367646.396308461675811No Hit
AACATTCAACGCTGTCGGTGAGT5181343.537865341197927No Hit
AAGCTGCCAGTTGAAGAACTGT5108763.488307067379929No Hit
TAGCAGCACGTAAATATTGGCG4283712.9249555406020393No Hit
TTCAAGTAATCCAGGATAGGCT3873582.6449151046558352No Hit
CCCCCACTGCTAAATTTGACTG3481932.377492978163428No Hit
TATTGCACTTGTCCCGGCCTGTAA2522051.7220783202353505No Hit
AACATTCAACGCTGTCGGTGAG2408301.6444088018170913No Hit
TAGCTTATCAGACTGATGTTGAC2398511.6377241021659723No Hit
TAGCTTATCAGACTGATGTTGACT2008421.3713671576404443No Hit
TATTGCACTTGTCCCGGCCTGTAT1845491.260117094907372No Hit
TATTGCACTTGTCCCGGCCTGT1507941.0296349327791656No Hit
TAGCTTATCAGACTGATGTTGA1397600.9542937928910712No Hit
TGAGGTAGTAGGTTGTATAGTT1357470.9268926696020623No Hit
TGTAAACATCCTTGACTGGAAGCT1271690.8683213176027808No Hit
AGCAGCATTGTACAGGGCTATGA1239110.8460754019098848No Hit
TATTGCACTTGTCCCGGCCTGTA1042400.7117600527401635No Hit
CGCCTGTCTGAGCGTCGCT897180.6126025365669799No Hit
ACTGGACTTGGAGTCAGAAGGC843900.5762224755443438No Hit
TGTAAACATCCCCGACTGGAAGCT784440.5356226551913794No Hit
CATAAAGTAGAAAGCACTACT783840.5352129698195028No Hit
TTCAAGTAATTCAGGATAGGTT661000.451336718017314No Hit
TGAGGTAGTAGATTGTATAGTT647580.4421734218663422No Hit
CAACGGAATCCCAAAAGCAGCTGT640500.4373391344781991No Hit
GCCTGTCTGAGCGTCGCT518420.35398181748038715No Hit
TGATGTAGTAGGTTGTATAGTT498660.34048951256658666No Hit
CCCATAAAGTAGAAAGCACTAC486280.3320363377268675No Hit
GGAATACCGGGTGCTGTAGGCTTT478280.32657386610184713No Hit
AACATTCAACGCTGTCGGTGAGTT469730.3207358495526065No Hit
CCCCCACTGCTAAATTTGACTGGCTT451570.3083360389638101No Hit
CATTGCACTTGTCTCGGTCTGA442220.30195177525206746No Hit
TCGTACGACTCTTAGCGG369910.25257785985141396No Hit
TGTAAACATCCTACACTCTCAGCT365730.24972371842734076No Hit
TTCTCACTACTGCACTTGACTAG352840.24092231102152656No Hit
CACCCGGCTGTGTGCACATGTGC350210.2391265234748011No Hit
ACCGGGTGCTGTAGGCTT346350.23649088091572873No Hit
ACTTGACTTGGAGTCAGAAGGC345880.2361699607077588No Hit
GGAATACCGGGTGCTGTAGGCTT344800.23543252703838105No Hit
CAACGGAATCCCAAAAGCAGCT335750.22925310601257667No Hit
AACCGGGCGGAAACACCA329500.22498555005552945No Hit
CTCCCACTGCTTCACTTGACTAG329170.22476022310099733No Hit
CTACGCCTGTCTGAGCGTCGCT328210.22410472650599486No Hit
TGAATACCGGGTGCTGTAGGCTTT323050.22058143230785668No Hit
AGCTCGGTCTGAGGCCCCTCAGT316850.21634801679846585No Hit
TACGCCTGTCTGAGCGTCGCT314640.21483900901205394No Hit
TCCTGTCTGAGCGTCGCT311220.21250380239235772No Hit
CCCATAAAGTAGAAAGCACT300470.2051636061462365No Hit
CTTCTCACTACTGCACTTGACTAG300380.20510215334045503No Hit
CCCCCCACTGCTAAATTTGACTGGCT282290.19275013937837757No Hit
TAGCTTATCAGACTGATGTTGACA279700.1909816641897772No Hit
TGAGGTAGTAGTTTGTACAGTT271050.18507536674522385No Hit
TTCAAGTAATCCAGGATAGGCTT267400.18258311406630826No Hit
TATTGCACTTGTCCCGGCCTGTAG260470.1778512480211343No Hit
ACGCCTGTCTGAGCGTCGCT259560.17722989187378824No Hit
ACCGGGTGCTGTAGGCTTA258690.17663584808456725No Hit
CCCCCCACTGCTAAATTTGACTGG257950.17613056945925284No Hit
TATTGCACTTGTCCCGGCCTGTT256560.17518146501440554No Hit
ACCGGGTGCTGTAGGCTTT254400.17370659767565005No Hit
ACCCCCCACTGCTAAATTTGACTG235900.16107463204279027No Hit
TGATGTAGTAGATTGTATAGTT235820.16102000732654007No Hit
GCGTTCGCGCTTTCCCCTG233430.1593880939285652No Hit
AGCAGCATTGTACAGGGCTATGT230440.15734649515871382No Hit
TGAATACCGGGTGCTGTAGGCTT227070.15504542898667395No Hit
CAACACCAGTCGATGGGCTGT223250.1524370987857267No Hit
TAGCAGCACGTAAATATTGGC220350.15045695282165678No Hit
ACCACAGGGTAGAACCACGGAC216550.1478622787997721No Hit
CAAAGAATTCTCCTTTTGGGCT213540.14580702385085811No Hit
TTCACAGTGGCTAAGTTCCG210820.1439497834983512No Hit
TTATAAAGCAATGAGACTGATT208080.14207888696678167No Hit
TCGTTTCCCGGCCAATGCACCA206790.14119806341724714No Hit
CAAAGAATTCTCCTTTTGGGCTT195420.13343452562018684No Hit
TGTAAACATCCCCGACTGGAAGC192290.1312973335968976No Hit
CAAAGTGCTGTTCGTGCAGGTAG188670.12882556518657584No Hit
CACTAGATTGTGAGCTCCTGGA185130.1264084214925043No Hit
CACTAGATTGTGAGCTCCTGGAA184670.12609432937406562No Hit
CGCGACCTCAGATCAGACGTGGCG179390.12248909810155212No Hit
TATTGCACTTGTCCCGGCCTGTAAT178050.1215741341043612No Hit
AGCAGCATTGTACAGGGCTATG175700.11996953306451145No Hit
AACGGAATCCCAAAAGCAGCTG172730.11794159047372262No Hit
TTCAAGTAATCCAGGATAGGC169970.11605703776309056No Hit
TGAGGTAGTAGTTTGTGCTGTT169870.1159887568677778No Hit
TATTGCACTTGTCCCGGCCTGTAAA169070.11544250970527575No Hit
TCGTTCGCGCTTTCCCCTG168930.11534691645183791No Hit
CTCCCACTGCTTCACTTGACTAGCCT168700.11518987039261858No Hit
CCAAGTCCTTCTGATCGAGGCCC163130.11138662452369807No Hit
TGAGGTAGTAGGTTGTGTGGTT161820.11049214479510099No Hit
CTCCCCACTGCTAAATTTGACTG158720.10837543704040556No Hit
GTCAGGATGGCCGAGCGGTCTAAGG157100.10726928653633894No Hit
ACTGGACTTGGAGTCAGAAGGCA156800.10706444385040065No Hit
CCCCCACTGCTAAATTTGACTGGCT150880.10302221484788553No Hit
CAAAGTGCTGTTCGTGCAGGT149610.10215504747741354No Hit
CGCGACCTCAGATCAGACGTGG149480.10206628231350695No Hit
CAACGGAATCCCAAAAGCAGCTGA149280.10192972052288143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)