FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7055018
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG93716313.28363726357608No Hit
CCCCCCACTGCTAAATTTGACTG4801316.8055248051812205No Hit
TTCAAGTAATCCAGGATAGGCT3188354.519265578060892No Hit
TAGCAGCACGTAAATATTGGCG2847844.03661620707417No Hit
AAGCTGCCAGTTGAAGAACTGT2545383.607900079064291No Hit
CCCCCACTGCTAAATTTGACTG1683092.385663651035334No Hit
AACATTCAACGCTGTCGGTGAGT1519502.153786142005591No Hit
AACATTCAACGCTGTCGGTGAG1207661.7117745128361117No Hit
TGAGGTAGTAGGTTGTATAGTT1104051.5649145048247926No Hit
TAGCTTATCAGACTGATGTTGAC908831.2882036587291485No Hit
CATAAAGTAGAAAGCACTACT824931.1692812123229168No Hit
TATTGCACTTGTCCCGGCCTGTAA800171.1341856250402196No Hit
TATTGCACTTGTCCCGGCCTGTAT678480.9616984676722298No Hit
TATTGCACTTGTCCCGGCCTGT658390.9332222823527877No Hit
TAGCTTATCAGACTGATGTTGACT657350.9317481542924482No Hit
TGTAAACATCCTTGACTGGAAGCT647170.9173187084710486No Hit
TGAGGTAGTAGATTGTATAGTT640640.9080628851691095No Hit
TTCAAGTAATTCAGGATAGGTT635190.9003378871605997No Hit
CAACGGAATCCCAAAAGCAGCTGT601680.8528397801394696No Hit
TCGTACGACTCTTAGCGG534870.7581412265709315No Hit
TAGCTTATCAGACTGATGTTGA516000.7313943068607337No Hit
CCCATAAAGTAGAAAGCACTAC462620.6557318493021563No Hit
AGCAGCATTGTACAGGGCTATGA418270.5928687921136416No Hit
GCCTGTCTGAGCGTCGCT391530.5549666917929905No Hit
TATTGCACTTGTCCCGGCCTGTA352390.4994884492144457No Hit
CATTGCACTTGTCTCGGTCTGA341240.48368409549061386No Hit
ACTGGACTTGGAGTCAGAAGGC322780.4575183224195884No Hit
CTCCCACTGCTTCACTTGACTAG286630.4062781980145196No Hit
TGTAAACATCCCCGACTGGAAGCT279710.39646957668995314No Hit
CCCCCACTGCTAAATTTGACTGGCTT268190.3801407735600391No Hit
TGAGGTAGTAGTTTGTACAGTT254420.3606227510688137No Hit
CCCATAAAGTAGAAAGCACT253200.35889348545956934No Hit
CACCCGGCTGTGTGCACATGTGC244620.3467319289617688No Hit
AACGGAATCCCAAAAGCAGCTG228870.3244073934325894No Hit
CAACGGAATCCCAAAAGCAGCT223300.31651230372480976No Hit
TGTAAACATCCTACACTCTCAGCT219690.3113953784384391No Hit
TAGCAGCACGTAAATATTGGC205130.29075758559368664No Hit
TTCTCACTACTGCACTTGACTAG202650.2872423571421079No Hit
CCCCCCACTGCTAAATTTGACTGGCT181160.2567817686645165No Hit
ACCGGGTGCTGTAGGCTT177510.2516081461450559No Hit
CAACACCAGTCGATGGGCTGT168930.23944658964725535No Hit
TGAGGTAGTAGTTTGTGCTGTT166810.23644163629348644No Hit
TTCAAGTAATCCAGGATAGGC166420.23588883827085913No Hit
TTCACAGTGGCTAAGTTCCGC163340.23152315132293072No Hit
TTCACAGTGGCTAAGTTCTGC147650.2092836616433863No Hit
TGATGTAGTAGGTTGTATAGTT146480.20762526757550442No Hit
CTTCTCACTACTGCACTTGACTAG144570.2049179746954579No Hit
CAAAGAATTCTCCTTTTGGGCT140340.1989222422961926No Hit
CGCCTGTCTGAGCGTCGCT137060.19427306918281426No Hit
TTCAAGTAATCCAGGATAGGCTT135460.1920051798592151No Hit
ACCCCCCACTGCTAAATTTGACTG133440.18914196958817114No Hit
CCCCCCACTGCTAAATTTGACTGG131640.18659059409912207No Hit
ACCGGGTGCTGTAGGCTTT122250.17328091863124942No Hit
CTCCCACTGCTTCACTTGACTAGCCT117930.16715761745753163No Hit
TGTCAGTTTGTCAAATACCCCA111330.15780257399768505No Hit
TGAGGTAGTAGGTTGTGTGGTT111240.15767500522323258No Hit
AGCTCGGTCTGAGGCCCCTCAGT111220.15764665660668759No Hit
ACCGGGTGCTGTAGGCTTA110990.15732064751642022No Hit
TAGCTTATCAGACTGATGTTGACA109940.15583234514780828No Hit
CAAAGAATTCTCCTTTTGGGCTT105450.14946808073345808No Hit
TGTCAGTTTGTCAAATACCCCAA102420.14517326532689215No Hit
ATCACATTGCCAGGGATTTCC101390.1437133115748252No Hit
CACTAGATTGTGAGCTCCTGGA101250.14351487125901027No Hit
TATTGCACTTGTCCCGGCCTGTT100580.1425651926047531No Hit
TGAGGTAGTAGGTTGTATAGT100000.1417430827249484No Hit
CAAAGTGCTGTTCGTGCAGGTAG99040.1403823491307889No Hit
AACATTCAACGCTGTCGGTGAGTT98190.13917753292762683No Hit
TGAGAACTGAATTCCATAGGCTGT97530.1382420285816422No Hit
CCCATAAAGTAGAAAGCACTA97450.13812863411546222No Hit
CAAAGTGCTGTTCGTGCAGGT96330.1365411115889428No Hit
TAGCAGCACGTAAATATTGGCGT95850.13586074479186303No Hit
TTATAAAGCAATGAGACTGATT95120.13482602028797092No Hit
AGAGGTAGTAGGTTGCATAGTT94910.13452835981424852No Hit
CGCGACCTCAGATCAGACGTGG93950.13316762622008904No Hit
AACCGGGCGGAAACACCA90520.12830583848262328No Hit
TCCCACTGCTTCACTTGACTAG89590.12698762781328127No Hit
TTCACAGTGGCTAAGTTCCG87400.12388345430160491No Hit
ACCACAGGGTAGAACCACGGAC86650.1228203811811678No Hit
TGATGTAGTAGATTGTATAGTT86330.12236680331644795No Hit
TCGTTTCCCGGCCAATGCACCA85780.12158721636146073No Hit
AGCAGCATTGTACAGGGCTATGT82720.11724987803007732No Hit
CACTAGATTGTGAGCTCCTGGAA81260.11518042902229307No Hit
TTCACAGTGGCTAAGTTCTG80100.11353620926268368No Hit
TGTAAACATCCTACACTCAGCT79930.11329524602205125No Hit
TATTGCACTTGTCCCGGCCTGTAG79730.11301175985660135No Hit
AATGACACGATCACTCCCGTTG77220.10945400848020516No Hit
TCCTGTCTGAGCGTCGCT74270.10527258753981916No Hit
CCCCCACTGCTAAATTTGACTGGCT73910.10476231244200937No Hit
CAAGGGAATCCCAAAAGCAGCTG72880.10330235868994239No Hit
CTACGCCTGTCTGAGCGTCGCT72140.10225345987777776No Hit
TGTAAACATCCCCGACTGGAAGC71770.10172901047169547No Hit
TAGCAGCACATCATGGTTTACA71740.10168648754687798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)