FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8019184
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG200447324.995972158763287No Hit
TAGCAGCACGTAAATATTGGCG4038925.036572299625498No Hit
TAGCTTATCAGACTGATGTTGAC3176913.9616374932910876No Hit
TCGTACGACTCTTAGCGG2173472.7103381092141046No Hit
AAGCTGCCAGTTGAAGAACTGT2142742.672017502030132No Hit
CCCCCCACTGCTAAATTTGACTG1981632.4711117739660295No Hit
TTCAAGTAATCCAGGATAGGCT1645802.052328516218109No Hit
TAGCTTATCAGACTGATGTTGA1392301.7362115646679264No Hit
AACATTCAACGCTGTCGGTGAG1143811.4263421315684988No Hit
TGAGGTAGTAGGTTGTATAGTT1122431.3996810648065938No Hit
AACATTCAACGCTGTCGGTGAGT1063111.3257084511341803No Hit
CCCCCACTGCTAAATTTGACTG1062681.3251722369757322No Hit
CATAAAGTAGAAAGCACTACT1006491.2551027635729521No Hit
TTCACAGTGGCTAAGTTCCGC831051.0363273869261511No Hit
TGAGAACTGAATTCCATGGGTT726700.9062019277771903No Hit
TAGCTTATCAGACTGATGTTGACT644580.8037974936103224No Hit
TATTGCACTTGTCCCGGCCTGT526650.65673764313177No Hit
CATTGCACTTGTCTCGGTCTGA513830.6407509791519935No Hit
AGCAGCATTGTACAGGGCTATGA485160.6049992118898881No Hit
TAGCAGCACGTAAATATTGGC461050.5749338087266734No Hit
TAGCTTATCAGACTGATGTTGACA429590.5357028844830097No Hit
TTCAAGTAATTCAGGATAGGTT417140.5201776140814327No Hit
CAACGGAATCCCAAAAGCAGCT394060.4913966308791518No Hit
CCCCCACTGCTAAATTTGACTGGCTT360360.44937240497287506No Hit
TGTAAACATCCTTGACTGGAAGCT348120.434109006602168No Hit
TGATGTAGTAGGTTGTATAGTT346180.43168980784079775No Hit
TGTAAACATCCTACACTCTCAGCT292000.36412682387634454No Hit
CTCCCACTGCTTCACTTGACTAGCCT287740.3588145626786965No Hit
TGAGGTAGTAGATTGTATAGTT266550.3323904277542453No Hit
TAGCAGCACGTAAATATTGGCGT262870.3278014321656667No Hit
TTCACAGTGGCTAAGTTCTGC259200.3232249066737962No Hit
TATTGCACTTGTCCCGGCCTGTA240450.29984347534612No Hit
CAACGGAATCCCAAAAGCAGCTGT237600.29628949778431324No Hit
TGAGAACTGAATTCCATAGGCTGT236350.29473073569580144No Hit
CACCCGGCTGTGTGCACATGTGC227890.28418103388075394No Hit
AACGGAATCCCAAAAGCAGCTG220760.27528985492788294No Hit
ACTGGACTTGGAGTCAGAAGGC210610.26263270676916756No Hit
TGATAACTGAATTCCATGGGTT205590.2563727182217044No Hit
CAACACCAGTCGATGGGCTGT205050.2556993329994673No Hit
AATCCTTGGAACCTAGGTGTGAGT191110.23831601818838427No Hit
TGAGGTAGTAGTTTGTGCTGTT178930.22312744039792576No Hit
TCAGTGCATCACAGAACTTTGT176780.22044636960568556No Hit
CCCATAAAGTAGAAAGCACT163660.20408560272466625No Hit
ACCGGGTGCTGTAGGCTT157980.19700258779446886No Hit
TGTAAACATCCCCGACTGGAAGCT140100.1747060548803968No Hit
TTCACCACCTTCTCCACCCAGC137440.1713890091560438No Hit
AGCAGCATTGTACAGGGCTATG132660.1654283029295749No Hit
CCCATAAAGTAGAAAGCACTAC124020.15465413937378167No Hit
TGGCTCAGTTCAGCAGGAAC121250.15119992258563963No Hit
CAAAGAATTCTCCTTTTGGGCT120280.14999032320495453No Hit
CGCGACCTCAGATCAGACGTGG117720.14679797844768244No Hit
TATTGCACTTGTCCCGGCCTGTAT115670.1442416086225232No Hit
TCAGTGCATCACAGAACTTTGTT115390.14389244591469655No Hit
ACCGGGTGCTGTAGGCTTT113330.1413236059928292No Hit
TTAATGCTAATCGTGATAGGGGTT113200.14116149473562398No Hit
TGAGGTAGTAGGTTGTGTGGTT112750.14060034038375976No Hit
AACATTCAACGCTGTCGGTGAGTT112220.13993942525823078No Hit
CGACGGAATCCCAAAAGCAGCTG111810.1394281512931989No Hit
ATCACATTGCCAGGGATTTCC109390.13641038788984017No Hit
CCCATAAAGTAGAAAGCACTA104840.1307364938876574No Hit
CAAAGAATTCTCCTTTTGGGCTT103370.1289033896715676No Hit
TAGCAGCACGTAAATATTGGCGA101220.1262223188793274No Hit
TGTAAACATCCCCGACTGGA101040.1259978571385817No Hit
TAGCAGCACATAATGGTTTGTG98400.12270575160764487No Hit
TTATAAAGCAATGAGACTGATT95580.11918918433596237No Hit
CAAAGTGCTGTTCGTGCAGGTAG94760.11816663640589865No Hit
TGAGGTAGTAGTTTGTACAGTT94390.11770524282769919No Hit
AGCAGCATTGTACAGGGCTATGT94160.117418430603413No Hit
TATTGCACTTGTCCCGGCCTGTAA94070.11730619973304016No Hit
CAAAGAATTCTCCTTTTGGGC93380.11644576306018169No Hit
ACCACAGGGTAGAACCACGGAC92840.1157723778379446No Hit
TGTAAACATCCTACACTCTCAGC92090.11483712058483757No Hit
TTCACAGTGGCTAAGTTCCG89420.11150760476377646No Hit
TCCCACTGCTTCACTTGACTAGCCT89050.11104621118557699No Hit
CACTAGATTGTGAGCTCCTGGA87810.10949991919377332No Hit
CCCCCCACTGCTAAATTTGACTGGCT86740.10816561884600727No Hit
CTACGGAATCCCAAAAGCAGCTG86600.10799103749209396No Hit
TGAGGTAGTAAGTTGTATTGTT85260.10632004453320937No Hit
TCTCACACAGAAATCGCACCCGT84550.1054346676669347No Hit
CTTCTCACTACTGCACTTGACTAG83100.10362650364426106No Hit
TCGGACGACTCTTAGCGG83090.10361403354755296No Hit
TGATGTAGTAGATTGTATAGTT83040.10355168306401251No Hit
TAGCTTATCAGACTGATGTTG82550.1029406483253159No Hit
TATTGCACTTGTCCCGGCCTGTT82160.10245431455370023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)