FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8235340
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG90989611.048675585950306No Hit
CCCCCCACTGCTAAATTTGACTG5084356.173819174436028No Hit
TTCAAGTAATCCAGGATAGGCT3771174.579252344165511No Hit
TAGCAGCACGTAAATATTGGCG3667304.453125189731086No Hit
AAGCTGCCAGTTGAAGAACTGT2936813.56610656026345No Hit
GCCTGTCTGAGCGTCGCT2219532.6951285557123326No Hit
CCCCCACTGCTAAATTTGACTG1766882.145485189439659No Hit
AACATTCAACGCTGTCGGTGAGT1603871.9475455779603512No Hit
AACATTCAACGCTGTCGGTGAG1396471.6957041239341668No Hit
TATTGCACTTGTCCCGGCCTGTAA1362241.654139355509305No Hit
TGAGGTAGTAGGTTGTATAGTT1340471.6277045027891988No Hit
TAGCTTATCAGACTGATGTTGAC1250501.5184558257461136No Hit
TATTGCACTTGTCCCGGCCTGTAT1191901.4472990793337008No Hit
TAGCTTATCAGACTGATGTTGACT1101081.337018265183951No Hit
TGTAAACATCCTTGACTGGAAGCT1073141.3030913113484082No Hit
CATAAAGTAGAAAGCACTACT923141.1209494689958155No Hit
TGAGGTAGTAGATTGTATAGTT891531.0825661114173792No Hit
TATTGCACTTGTCCCGGCCTGT840781.0209414547547522No Hit
TTCAAGTAATTCAGGATAGGTT755890.9178613147726747No Hit
TAGCTTATCAGACTGATGTTGA720100.8744022711873461No Hit
CAACGGAATCCCAAAAGCAGCTGT568840.6907304373589919No Hit
TATTGCACTTGTCCCGGCCTGTA548500.6660320035359802No Hit
AGCAGCATTGTACAGGGCTATGA515220.6256208001126852No Hit
CCCATAAAGTAGAAAGCACTAC474650.5763575031510539No Hit
TGTAAACATCCCCGACTGGAAGCT452530.5494976527987916No Hit
CATTGCACTTGTCTCGGTCTGA346300.4205048000446855No Hit
TGAGAACTGAATTCCATAGGCTGT343300.4168619631976336No Hit
ACTGGACTTGGAGTCAGAAGGC337540.4098677164512941No Hit
TGAGGTAGTAGTTTGTACAGTT333960.40552059781381217No Hit
CACCCGGCTGTGTGCACATGTGC329080.39959491654260787No Hit
CCCCCACTGCTAAATTTGACTGGCTT326470.3964256484856727No Hit
TGTAAACATCCTACACTCTCAGCT324930.3945556589041861No Hit
TCGTACGACTCTTAGCGG303490.36852151823725554No Hit
TGATGTAGTAGGTTGTATAGTT303030.36796294992070755No Hit
TGAGAACTGAATTCCATGGGTT282220.3426938049916579No Hit
TTCTCACTACTGCACTTGACTAG279780.3397309643560557No Hit
CAAAGAATTCTCCTTTTGGGCT262280.31848108274825326No Hit
TAGCAGCACGTAAATATTGGC257500.3126768293719506No Hit
CTCCCACTGCTTCACTTGACTAG242600.29458407303159306No Hit
CCCATAAAGTAGAAAGCACT223360.27122134605250053No Hit
TTAATGCTAATCGTGATAGGGGTT222050.26963064062928793No Hit
CAACGGAATCCCAAAAGCAGCT208430.2530921613436725No Hit
TGATGTAGTAGATTGTATAGTT206420.2506514606561478No Hit
CTTCTCACTACTGCACTTGACTAG196750.23890938321915065No Hit
TTCACAGTGGCTAAGTTCCGC194600.23629868347876348No Hit
TGAGGTAGTAGTTTGTGCTGTT193830.23536368868802018No Hit
AACGGAATCCCAAAAGCAGCTG188370.22873372562638578No Hit
TGTCAGTTTGTCAAATACCCCA184200.22367018240898373No Hit
AACCGGGCGGAAACACCA177500.2155345134505679No Hit
TGTCAGTTTGTCAAATACCCCAA175710.213360954131827No Hit
CAAAGAATTCTCCTTTTGGGCTT175240.21279024302578883No Hit
TTCACAGTGGCTAAGTTCTGC173580.21077453997042014No Hit
TTCAAGTAATCCAGGATAGGC173210.21032525675928376No Hit
CCCCCCACTGCTAAATTTGACTGGCT165570.2010481655887917No Hit
ACCCCCCACTGCTAAATTTGACTG165170.20056245400918482No Hit
TAGCTTATCAGACTGATGTTGACA162980.19790318311083696No Hit
TTCAAGTAATCCAGGATAGGCTT162860.1977574696369549No Hit
CAACACCAGTCGATGGGCTGT161670.1963124776876243No Hit
AGCTCGGTCTGAGGCCCCTCAGT159720.19394463373704063No Hit
TATTGCACTTGTCCCGGCCTGTAG147710.17936114355934304No Hit
ATCACATTGCCAGGGATTTCC142040.17247618191841502No Hit
TATTGCACTTGTCCCGGCCTGTT140230.17027833702069373No Hit
CCCCCCACTGCTAAATTTGACTGG137750.16726692522713088No Hit
TTATAAAGCAATGAGACTGATT136050.1652026510138015No Hit
TGTAAACATCCCCGACTGGAAGC135010.1639398009068235No Hit
CGCCTGTCTGAGCGTCGCT125160.15197915325900327No Hit
CACTAGATTGTGAGCTCCTGGA122900.1492348828342242No Hit
TCGTTTCCCGGCCAATGCACCA121440.14746203556865897No Hit
TAGCAGCACGTAAATATTGGCGT120400.146199185461681No Hit
CACTAGATTGTGAGCTCCTGGAA117450.14261706256208No Hit
ACCGGGTGCTGTAGGCTT117010.1420827798245124No Hit
AACATTCAACGCTGTCGGTGAGTT112050.13605995623738668No Hit
TATTGCACTTGTCCCGGCCTGTAAT110820.1345663931300954No Hit
TGTAAACATCCTACACTCAGCT110010.13358282718139142No Hit
CTCCCACTGCTTCACTTGACTAGCCT107860.13097212744100425No Hit
TTCACAGTGGCTAAGTTCCG104890.1273657189624229No Hit
AGCAGCATTGTACAGGGCTATGT104410.12678286506689465No Hit
TGAGGTAGTAGGTTGTATAGT103130.1252285880121525No Hit
CCCATAAAGTAGAAAGCACTA102080.12395359511568435No Hit
TGTCAGTTTGTCAAATACCCC101640.12341931237811676No Hit
ACCGGGTGCTGTAGGCTTA100920.12254503153482431No Hit
TGAGGTAGTAAGTTGTATTGTT100560.12210789111317809No Hit
ATGCACCTGGGCAAGGATTCTGA99770.12114861074345443No Hit
ATCACATTGCCAGGGATTTCCA99540.12086932658518046No Hit
TATTGCACTTGTCCCGGCCTGTAAA99240.12050504290047528No Hit
ACCGGGTGCTGTAGGCTTT98050.11906005095114469No Hit
CAAAGTGCTGTTCGTGCAGGTAG98000.11899933700369385No Hit
TAGCAGCACATCATGGTTTACA96940.11771220131773551No Hit
TCGTTTCCCGGGCGGCGCACCA92270.1120415186258248No Hit
CTACGCCTGTCTGAGCGTCGCT92030.11175009167806065No Hit
TATTGCACTTGTCCCGGCCTGTTT91050.11056009830802373No Hit
AGAGGTAGTAGGTTGCATAGTT89630.10883582220041917No Hit
TTCACAGTGGCTAAGTTCTG89060.10814368319947933No Hit
TGAGGTAGTAGGTTGTGTGGTT88540.10751225814599032No Hit
GGAATACCGGGTGCTGTAGGCTTT87710.10650440661830599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)