FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8957862
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6657157.4316282166436585No Hit
CCCCCCACTGCTAAATTTGACTG5064905.654139347089741No Hit
AAGCTGCCAGTTGAAGAACTGT4107334.585167755430928No Hit
TTCAAGTAATCCAGGATAGGCT3608584.02839427533043No Hit
TAGCAGCACGTAAATATTGGCG3306603.6912825850632665No Hit
AACATTCAACGCTGTCGGTGAGT2340842.6131681867838554No Hit
CCCCCACTGCTAAATTTGACTG2039432.2766928090653775No Hit
GCCTGTCTGAGCGTCGCT1605821.7926375735638704No Hit
TAGCTTATCAGACTGATGTTGAC1541901.72128126108663No Hit
AACATTCAACGCTGTCGGTGAG1341531.4976006551563308No Hit
TGAGGTAGTAGGTTGTATAGTT1266731.4140985873638152No Hit
TATTGCACTTGTCCCGGCCTGT1062771.1864103287145973No Hit
TAGCTTATCAGACTGATGTTGACT976991.0906508718263355No Hit
TGTAAACATCCTTGACTGGAAGCT971261.0842542562053312No Hit
TATTGCACTTGTCCCGGCCTGTAA932501.040985002894664No Hit
CATAAAGTAGAAAGCACTACT881590.9841522452567366No Hit
AACCGGGCGGAAACACCA839490.9371544236783287No Hit
TATTGCACTTGTCCCGGCCTGTAT818680.9139234339622557No Hit
TAGCTTATCAGACTGATGTTGA775620.8658539280913236No Hit
AGCAGCATTGTACAGGGCTATGA764220.853127677117598No Hit
TGAGGTAGTAGATTGTATAGTT706440.7886256787612937No Hit
CGCCTGTCTGAGCGTCGCT652010.7278634120507773No Hit
TTAATGCTAATCGTGATAGGGGTT642920.7177159014059382No Hit
TATTGCACTTGTCCCGGCCTGTA577740.6449530032947594No Hit
ACTGGACTTGGAGTCAGAAGGC530190.5918711406806669No Hit
TTCAAGTAATTCAGGATAGGTT527170.5884998005104343No Hit
TCGTACGACTCTTAGCGG477320.5328503609455024No Hit
CATTGCACTTGTCTCGGTCTGA450360.502753893730446No Hit
ACCGGGTGCTGTAGGCTT435170.48579672247685884No Hit
TGTAAACATCCCCGACTGGAAGCT403310.45023020001870984No Hit
CAACGGAATCCCAAAAGCAGCTGT393070.438798900898451No Hit
TGTAAACATCCTACACTCTCAGCT374740.4183364289380658No Hit
CCCATAAAGTAGAAAGCACT365250.40774238317134154No Hit
ACCGGGTGCTGTAGGCTTT319080.35620106672775265No Hit
CCCATAAAGTAGAAAGCACTAC311700.34796249372897237No Hit
ACCGGGTGCTGTAGGCTTA292250.3262497234273089No Hit
TGAGGTAGTAGTTTGTACAGTT286100.3193842459283253No Hit
CAACACCAGTCGATGGGCTGT263250.29387592709063837No Hit
GCGTTCGCGCTTTCCCCTG260850.2911967163593277No Hit
TTCACAGTGGCTAAGTTCCG257020.2869211425672778No Hit
TGAGGTAGTAGTTTGTGCTGTT234860.26218309681484264No Hit
CAACGGAATCCCAAAAGCAGCT229140.25579764457188553No Hit
TTCTCACTACTGCACTTGACTAG220900.24659902106105228No Hit
CTCCCACTGCTTCACTTGACTAG220710.24638691687815686No Hit
TACGCCTGTCTGAGCGTCGCT220500.24615248593916716No Hit
CACCCGGCTGTGTGCACATGTGC213160.2379585664525754No Hit
AACATTCAACGCTGTCGGTGAGTT204150.2279003628321133No Hit
CTACGCCTGTCTGAGCGTCGCT197660.22065533047952737No Hit
AACGGAATCCCAAAAGCAGCTG197450.22042089954053767No Hit
TGTAAACATCCTACACTCAGCT193310.2157992610290268No Hit
TTCAAGTAATCCAGGATAGGCTT182150.2033409311284322No Hit
CCCCCACTGCTAAATTTGACTGGCTT178310.19905419395833515No Hit
AGCTCGGTCTGAGGCCCCTCAGT177880.19857416870230865No Hit
CAAAGAATTCTCCTTTTGGGCT176060.1965424338977314No Hit
TTCAAGTAATCCAGGATAGGC174080.19433208504440008No Hit
TAGCAGCACGTAAATATTGGC173340.19350599506891264No Hit
CGCGACCTCAGATCAGACGTGG169750.1894983423499938No Hit
CTTCTCACTACTGCACTTGACTAG168890.1885382918379408No Hit
ACCACAGGGTAGAACCACGGAC165220.18444133209464492No Hit
TGTCAGTTTGTCAAATACCCCA164320.1834366280704034No Hit
TTATAAAGCAATGAGACTGATT160460.1791275641442121No Hit
TGTCAGTTTGTCAAATACCCCAA159050.17755352783956707No Hit
TCCTGTCTGAGCGTCGCT159000.17749771094933145No Hit
TATTGCACTTGTCCCGGCCTGTT157710.17605763518125195No Hit
ACGCCTGTCTGAGCGTCGCT154680.17267513163297224No Hit
TGAGAACTGAATTCCATAGGCTGT153120.17093364465762032No Hit
TTCACAGTGGCTAAGTTCTG145860.16282903219540557No Hit
AGCAGCATTGTACAGGGCTATG145840.1628067054393113No Hit
TTCACAGTGGCTAAGTTCTGC140580.1569347685865221No Hit
TAGCTTATCAGACTGATGTTGACA140350.15667801089143818No Hit
AGCAGCATTGTACAGGGCTATGT136110.15194473859945598No Hit
CCCCCCACTGCTAAATTTGACTGG135380.15112981200201567No Hit
ATCCCGGACGAGCCCCCA134910.15060513323380065No Hit
CAAAGAATTCTCCTTTTGGGCTT134020.14961159258760628No Hit
CCCCCCACTGCTAAATTTGACTGGCT131110.1463630495758921No Hit
CAAAGTGCTGTTCGTGCAGGTAG131090.14634072281979785No Hit
CAAAGTGCTGTTCGTGCAGGT130810.14602814823447827No Hit
TGAGGTAGTAGGTTGTGTGGTT128790.14377314586895845No Hit
TGAGGTAGTAGGTTGTATAGT128220.1431368333202722No Hit
AATGACACGATCACTCCCGTTG125420.14001108746707638No Hit
AGAGGTAGTAGGTTGCATAGTT120730.13477546316297348No Hit
TAGCAGCACGTAAATATTGGCGT116790.13037709221240515No Hit
CACTAGATTGTGAGCTCCTGGA115920.12940587832230502No Hit
TGTAAACATCCCCGACTGGAAGC114200.12748577729819907No Hit
ACTGGACTTGGAGTCAGAAGG112220.12527542844486775No Hit
CGCGACCTCAGATCAGACGTGGCG107730.12026307170170739No Hit
GGGCTACGCCTGTCTGAGCGTCGCT106820.11924720429941876No Hit
ACCCCCCACTGCTAAATTTGACTG105590.11787410879962207No Hit
TATTGCACTTGTCCCGGCCTGTAG102970.11494930375127459No Hit
TGATGTAGTAGGTTGTATAGTT102260.1141567039099285No Hit
TGAGAACTGAATTCCATGGGTT101140.1129064055686502No Hit
AGCTACATTGTCTGCTGGGTTT100600.1123035831541053No Hit
TCGTTTCCCGGCCAATGCACCA100170.1118235578980788No Hit
TTCACAGTGGCTAAGTTCCGC97530.10887642609363707No Hit
CCCATAAAGTAGAAAGCACTA97340.10866432191074164No Hit
ACTGGACTTGGAGTCAGAAGGCA90340.10084995727775221No Hit
ATCACATTGCCAGGGATTTCC89610.10003503068031189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)