FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5560918
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5489879.872236922033377No Hit
CCCCCCACTGCTAAATTTGACTG3592606.460444120916726No Hit
TAGCAGCACGTAAATATTGGCG2404084.323171102325191No Hit
TTCAAGTAATCCAGGATAGGCT2263134.069705757214906No Hit
AAGCTGCCAGTTGAAGAACTGT1483652.6679947447525754No Hit
AACATTCAACGCTGTCGGTGAGT1360702.446898156023879No Hit
CCCCCACTGCTAAATTTGACTG1269902.283615762721191No Hit
AACATTCAACGCTGTCGGTGAG1050601.8892564141388166No Hit
TAGCTTATCAGACTGATGTTGAC962121.7301459938808665No Hit
TATTGCACTTGTCCCGGCCTGTAA933531.6787336191614406No Hit
TAGCTTATCAGACTGATGTTGACT738401.3278383173425683No Hit
TATTGCACTTGTCCCGGCCTGTAT731601.315610120487301No Hit
TGTAAACATCCTTGACTGGAAGCT711361.279213252200446No Hit
TGAGGTAGTAGGTTGTATAGTT682471.2272613982079936No Hit
GCCTGTCTGAGCGTCGCT545510.9809711274289605No Hit
TAGCTTATCAGACTGATGTTGA476810.8574303739058912No Hit
CATAAAGTAGAAAGCACTACT456990.8217887766012735No Hit
TATTGCACTTGTCCCGGCCTGT446590.8030868284696879No Hit
TGAGGTAGTAGATTGTATAGTT441970.7947788476650798No Hit
CCCCCACTGCTAAATTTGACTGGCTT433690.7798892197295483No Hit
TTCAAGTAATTCAGGATAGGTT427650.7690277036992813No Hit
CAACGGAATCCCAAAAGCAGCT425180.7645859910180298No Hit
AGCAGCATTGTACAGGGCTATGA396760.7134793212199856No Hit
TGTAAACATCCCCGACTGGAAGCT358610.6448755403334485No Hit
TATTGCACTTGTCCCGGCCTGTA351050.6312806626531806No Hit
CAACGGAATCCCAAAAGCAGCTGT341040.6132800375765296No Hit
AACCGGGCGGAAACACCA266330.4789317159504959No Hit
ACTGGACTTGGAGTCAGAAGGC262970.47288954809259914No Hit
TTCTCACTACTGCACTTGACTAG244640.43992736451067976No Hit
CAAAGAATTCTCCTTTTGGGCT225900.4062278925889574No Hit
TGTAAACATCCTACACTCTCAGCT221810.3988729918333628No Hit
CATTGCACTTGTCTCGGTCTGA217410.3909606291623074No Hit
CCCCCCACTGCTAAATTTGACTGGCT206210.3708200696359846No Hit
CCCATAAAGTAGAAAGCACTAC199260.3583221331442039No Hit
TGAGGTAGTAGTTTGTACAGTT193020.3471009642652526No Hit
CTTCTCACTACTGCACTTGACTAG183090.32924420032807533No Hit
TAGCAGCACGTAAATATTGGC181690.32672663038728494No Hit
TCGTTTCCCGGCCAATGCACCA170670.3069097584247781No Hit
CACCCGGCTGTGTGCACATGTGC168920.30376279599879014No Hit
CCCATAAAGTAGAAAGCACT161500.29041967531260127No Hit
CTCCCACTGCTTCACTTGACTAG157660.283514340617862No Hit
TAGCTTATCAGACTGATGTTGACA148930.26781549377279074No Hit
CAAAGAATTCTCCTTTTGGGCTT142610.2564504637543657No Hit
GGAATACCGGGTGCTGTAGGCTTT139220.2503543479691662No Hit
AACGGAATCCCAAAAGCAGCTG139090.25012057361752144No Hit
GTCAGGATGGCCGAGCGGTCTAAGG130720.23506910190008198No Hit
TGTCAGTTTGTCAAATACCCCA122500.22028736981915575No Hit
TGTCAGTTTGTCAAATACCCCAA121930.2192623592004054No Hit
TCGTACGACTCTTAGCGG120470.21663689340500975No Hit
CAACGGAATCCCAAAAGCAGCTGA117490.21127806595961315No Hit
TTCACAGTGGCTAAGTTCTGC116810.21005524627408642No Hit
TGAGGTAGTAGTTTGTGCTGTT114620.20611704758099295No Hit
TGTAAACATCCCCGACTGGAAGC113910.2048402799681635No Hit
AGCTCGGTCTGAGGCCCCTCAGT113770.20458852297408453No Hit
ACCCCCCACTGCTAAATTTGACTG112280.20190910925138617No Hit
CCCCCCACTGCTAAATTTGACTGG105610.1899146867477636No Hit
AACATTCAACGCTGTCGGTGAGTT104640.18817037043164456No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC102470.18426813702341951No Hit
CTCCCACTGCTTCACTTGACTAGCCT101590.18268566448920845No Hit
GGAATACCGGGTGCTGTAGGCTT98950.1779382468865752No Hit
TTCAAGTAATCCAGGATAGGCTT98020.17626586114019305No Hit
TTCAAGTAATCCAGGATAGGC96180.17295705493229716No Hit
TATTGCACTTGTCCCGGCCTGTAG93710.1685153422510456No Hit
GGGAATACCGGGTGCTGTAGGCTTT93530.16819165468722969No Hit
ATCACATTGCCAGGGATTTCC92240.16587189381321574No Hit
TTCACAGTGGCTAAGTTCCG92110.16563811946157092No Hit
TTCACAGTGGCTAAGTTCCGC88770.1596319168885425No Hit
CACTAGATTGTGAGCTCCTGGAA88580.15929024668229239No Hit
AATGCACCCGGGCAAGGATTCT87740.15777970471781816No Hit
CTACGCCTGTCTGAGCGTCGCT86440.15544196120137No Hit
ATGCACCTGGGCAAGGATTCTGA86160.15493844721321193No Hit
TATTGCACTTGTCCCGGCCTGTT85110.15305026975761915No Hit
CACTAGATTGTGAGCTCCTGGA82930.1491300537069599No Hit
CAACACCAGTCGATGGGCTGT81910.14729582417866977No Hit
TATTGCACTTGTCCCGGCCTGTAAA81350.14628879620235363No Hit
CCCCCACTGCTAAATTTGACTGGCT78590.1413255868905098No Hit
GGGAATACCGGGTGCTGTAGGCTT76730.13798081539774548No Hit
TATTGCACTTGTCCCGGCCTGTAAT75240.13530140167504717No Hit
TAGCAGCACGTAAATATTGGCGT72330.13006845272669007No Hit
ACTGGACTTGGAGTCAGAAGGCA71710.12895352889576864No Hit
ATCACATTGCCAGGGATTTCCA71680.12889958096846602No Hit
ACTGGACTTGGAGTCAGAAGGCT69370.12474559056616193No Hit
TGAGAACTGAATTCCATAGGCTGT68600.12336092709872722No Hit
AGCAGCATTGTACAGGGCTATGT67460.12131090586122652No Hit
CGCCTGTCTGAGCGTCGCT66410.11942272840563374No Hit
TAGCAGCACATCATGGTTTACA66300.11922491933885737No Hit
TTATAAAGCAATGAGACTGATT63750.11463934551813207No Hit
TGTAAACATCCTACACTCAGCT63250.11374021339642124No Hit
CGCGACCTCAGATCAGACGTGGCG62930.1131647688385263No Hit
TGATGTAGTAGGTTGTATAGTT62790.11291301184444727No Hit
CCCATAAAGTAGAAAGCACTA62620.11260730692306557No Hit
ACCGGGTGCTGTAGGCTT61310.11025158076418318No Hit
TGAGGTAGTAGGTTGTGTGGTT60400.10861516030266946No Hit
CCCCCACTGCTAAATTTGACTGGCTA60130.10812962895694558No Hit
TTCACAGTGGCTAAGTTCTG59900.1077160281809586No Hit
TATTGCACTTGTCCCGGCCTGTTT59520.10703268776845838No Hit
AGAGGTAGTAGGTTGCATAGTT59350.10672698284707668No Hit
AACACCCTGATTGCTCCTATCTGATT58960.10602565979214224No Hit
TGTAAACATCCTTGACTGGAAGC57380.10318440228753599No Hit
CAAAGAATTCTCCTTTTGGGC56540.10167386032306176No Hit
TTAATGCTAATCGTGATAGGGGTT56450.10151201654115383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)