FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7069306
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG79258711.211666321984081No Hit
CCCCCCACTGCTAAATTTGACTG4274316.046293653153506No Hit
AAGCTGCCAGTTGAAGAACTGT2651923.7513159000331857No Hit
TAGCAGCACGTAAATATTGGCG2600133.6780555262426047No Hit
TTCAAGTAATCCAGGATAGGCT2409143.407887563503405No Hit
CCCCCACTGCTAAATTTGACTG1905652.6956677218386074No Hit
GCCTGTCTGAGCGTCGCT1845092.610001604117858No Hit
AACATTCAACGCTGTCGGTGAGT1475562.087277025495855No Hit
TAGCTTATCAGACTGATGTTGAC1385731.9602065605874182No Hit
TAGCTTATCAGACTGATGTTGACT1018271.4404101336114181No Hit
AACATTCAACGCTGTCGGTGAG1015291.4361947268939836No Hit
TGAGGTAGTAGGTTGTATAGTT942771.3336103996629938No Hit
AACCGGGCGGAAACACCA766631.0844487422103386No Hit
TCCTGTCTGAGCGTCGCT748791.059212884546234No Hit
TGTAAACATCCTTGACTGGAAGCT732911.036749576266751No Hit
TATTGCACTTGTCCCGGCCTGTAA710611.0052047541866203No Hit
TAGCTTATCAGACTGATGTTGA635500.8989567009830951No Hit
TATTGCACTTGTCCCGGCCTGTAT601870.851384845980638No Hit
CATAAAGTAGAAAGCACTACT554970.7850416999914842No Hit
TGAGGTAGTAGATTGTATAGTT532240.7528886145259521No Hit
TCGTACGACTCTTAGCGG493850.6985834253037002No Hit
AGCAGCATTGTACAGGGCTATGA487470.6895584941435553No Hit
TATTGCACTTGTCCCGGCCTGT483380.6837729191521771No Hit
CAACGGAATCCCAAAAGCAGCTGT470100.6649874825053548No Hit
TTCAAGTAATTCAGGATAGGTT386250.5463761223520386No Hit
ACTGGACTTGGAGTCAGAAGGC362580.5128933448346981No Hit
TGTAAACATCCCCGACTGGAAGCT359910.5091164535811578No Hit
TATTGCACTTGTCCCGGCCTGTA326160.4613748506571932No Hit
CAACGGAATCCCAAAAGCAGCT320590.4534957179672234No Hit
CCCATAAAGTAGAAAGCACTAC299170.42319571397814726No Hit
CATTGCACTTGTCTCGGTCTGA278770.3943385673218842No Hit
CCCCCACTGCTAAATTTGACTGGCTT227600.3219552244590912No Hit
TTCTCACTACTGCACTTGACTAG214190.30298589423063593No Hit
CCCATAAAGTAGAAAGCACT209730.2966769298146098No Hit
AACGGAATCCCAAAAGCAGCTG201830.2855018583153707No Hit
TGTAAACATCCTACACTCTCAGCT196850.2784573195728124No Hit
TGAGGTAGTAGTTTGTACAGTT194950.2757696441489447No Hit
TAGCAGCACGTAAATATTGGC188570.26674471298879976No Hit
CACCCGGCTGTGTGCACATGTGC176860.25018014498169977No Hit
TAGCTTATCAGACTGATGTTGACA169250.2394152976261036No Hit
CTTCTCACTACTGCACTTGACTAG168900.23892019952170696No Hit
CAAAGAATTCTCCTTTTGGGCT165300.23382776187648407No Hit
CAACACCAGTCGATGGGCTGT153710.2174329417908915No Hit
CGCCTGTCTGAGCGTCGCT147190.20820997138898784No Hit
TGAGGTAGTAGTTTGTGCTGTT139370.19714806517075367No Hit
CCCCCCACTGCTAAATTTGACTGGCT136950.19372481542035386No Hit
CAAAGAATTCTCCTTTTGGGCTT132030.18676515063854926No Hit
TGTCAGTTTGTCAAATACCCCAA130850.1850959627437262No Hit
CTCCCACTGCTTCACTTGACTAG129590.1833136095678982No Hit
ACCGGGTGCTGTAGGCTT126820.1793952617131017No Hit
TTCAAGTAATCCAGGATAGGCTT126450.17887187228845378No Hit
TTCACAGTGGCTAAGTTCTGC121860.17237901429079458No Hit
CTACGCCTGTCTGAGCGTCGCT121060.17124736148074507No Hit
TGTCAGTTTGTCAAATACCCCA116930.16540520384886437No Hit
TTCACAGTGGCTAAGTTCCGC110820.1567622055121111No Hit
CCCCCCACTGCTAAATTTGACTGG108980.15415940404899717No Hit
AACATTCAACGCTGTCGGTGAGTT108800.15390478216673603No Hit
GGAATACCGGGTGCTGTAGGCTTT107760.15243363351367165No Hit
CACTAGATTGTGAGCTCCTGGA106140.15014203657332134No Hit
TGTAAACATCCCCGACTGGAAGC104500.1478221483127198No Hit
TAGCAGCACGTAAATATTGGCGT100930.14277214764787377No Hit
AATGCACCCGGGCAAGGATTCT100560.14224875822322589No Hit
ACCGGGTGCTGTAGGCTTT100550.14223461256310024No Hit
TTCAAGTAATCCAGGATAGGC100340.14193755370046224No Hit
ACCCCCCACTGCTAAATTTGACTG99180.14029665712589043No Hit
TTATAAAGCAATGAGACTGATT96890.13705730095712365No Hit
CAACGGAATCCCAAAAGCAGCTGA94850.13417158629149734No Hit
CACTAGATTGTGAGCTCCTGGAA90740.12835771997986792No Hit
AGCAGCATTGTACAGGGCTATGT89580.12671682340529608No Hit
AGCTCGGTCTGAGGCCCCTCAGT86980.1230389517726351No Hit
ACTGGACTTGGAGTCAGAAGGCA86750.12271360158974587No Hit
TGTAAACATCCTACACTCAGCT86230.12197802726321366No Hit
CTCCCACTGCTTCACTTGACTAGCCT85190.12050687861014928No Hit
ACCGGGTGCTGTAGGCTTA84810.11996934352537576No Hit
CGCGACCTCAGATCAGACGTGGCG84730.11985617824437081No Hit
TATTGCACTTGTCCCGGCCTGTT84040.11888012769570308No Hit
ACTGGACTTGGAGTCAGAAGGCT82960.11735239640213622No Hit
ACCACAGGGTAGAACCACGGAC82520.11672998735660899No Hit
ATCACATTGCCAGGGATTTCC81980.11596612170982554No Hit
TTCACAGTGGCTAAGTTCCG81560.11537200398454954No Hit
TGAGGTAGTAGGTTGTGTGGTT80380.11370281608972649No Hit
GGAATACCGGGTGCTGTAGGCTT79550.11252872629930011No Hit
TACGCCTGTCTGAGCGTCGCT78950.11167998669176296No Hit
CGCGACCTCAGATCAGACGTGG78460.11098684934560761No Hit
TATTGCACTTGTCCCGGCCTGTAG75120.10626219886365083No Hit
TGAGGTAGTAGGTTGTATAGT73600.10411205852455672No Hit
CAAAGTGCTGTTCGTGCAGGTAG72350.10234385100885435No Hit
ACTGGACTTGGAGTCAGAAGG72170.1020892291265932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)