FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5306792
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG3785027.132406923052572No Hit
GCCTGTCTGAGCGTCGCT2635154.965617646216396No Hit
AAGCTGCCAGTTGAAGAACTGT2234264.210189508086995No Hit
CCCCCCACTGCTAAATTTGACTG2021453.809175109934589No Hit
TTCAAGTAATCCAGGATAGGCT1685233.175609671530371No Hit
TAGCAGCACGTAAATATTGGCG1581202.979577869266404No Hit
AACATTCAACGCTGTCGGTGAGT1241382.3392286714836383No Hit
CCCCCACTGCTAAATTTGACTG943521.7779479580130522No Hit
TAGCTTATCAGACTGATGTTGAC780061.4699275946748998No Hit
AACATTCAACGCTGTCGGTGAG689071.2984680763821155No Hit
TGAGGTAGTAGGTTGTATAGTT580251.0934101053894707No Hit
TATTGCACTTGTCCCGGCCTGT576941.0871728155164173No Hit
TAGCTTATCAGACTGATGTTGACT494090.931052130929571No Hit
CGCCTGTCTGAGCGTCGCT492280.9276414074642457No Hit
TGTAAACATCCTTGACTGGAAGCT490810.9248713723846722No Hit
TATTGCACTTGTCCCGGCCTGTAA445700.8398670986162639No Hit
AACCGGGCGGAAACACCA436950.8233787945711835No Hit
TAGCTTATCAGACTGATGTTGA425970.8026883284666142No Hit
CATAAAGTAGAAAGCACTACT416220.7843156468163817No Hit
TATTGCACTTGTCCCGGCCTGTAT411400.7752329467595489No Hit
AGCAGCATTGTACAGGGCTATGA405700.7644919944101822No Hit
TCGTACGACTCTTAGCGG348530.6567621267236402No Hit
TGAGGTAGTAGATTGTATAGTT335390.6320014049919425No Hit
ACCGGGTGCTGTAGGCTT328050.6181700733701265No Hit
ACTGGACTTGGAGTCAGAAGGC289640.5457911295562367No Hit
TATTGCACTTGTCCCGGCCTGTA275390.5189387486828201No Hit
TTCAAGTAATTCAGGATAGGTT269680.5081789525574019No Hit
TCCTGTCTGAGCGTCGCT260070.4900700837718908No Hit
TGTAAACATCCCCGACTGGAAGCT251850.4745804998575411No Hit
CATTGCACTTGTCTCGGTCTGA236640.44591911648317856No Hit
CAACGGAATCCCAAAAGCAGCTGT216390.40776046997884974No Hit
ACCGGGTGCTGTAGGCTTT202180.3809834642096393No Hit
TTAATGCTAATCGTGATAGGGGTT188730.3556385854203443No Hit
CCCATAAAGTAGAAAGCACTAC183410.3456136965609355No Hit
ACCGGGTGCTGTAGGCTTA174170.3282020474893307No Hit
CCCATAAAGTAGAAAGCACT170980.32219088292889564No Hit
TTCTCACTACTGCACTTGACTAG168740.31796987709335506No Hit
TGTAAACATCCTACACTCTCAGCT165570.3119964000850231No Hit
TACGCCTGTCTGAGCGTCGCT152670.28768792897856177No Hit
GCGTTCGCGCTTTCCCCTG151560.28559626983684305No Hit
CTACGCCTGTCTGAGCGTCGCT147360.2776818838952045No Hit
CTCCCACTGCTTCACTTGACTAG134470.2533922565647947No Hit
TGAGGTAGTAGTTTGTACAGTT132160.2490393442968935No Hit
CACCCGGCTGTGTGCACATGTGC129990.2449502448937136No Hit
ACGCCTGTCTGAGCGTCGCT122620.231062381943743No Hit
CAACACCAGTCGATGGGCTGT118480.22326105865841359No Hit
TGAGGTAGTAGTTTGTGCTGTT116720.219944554073346No Hit
ATCCCGGACGAGCCCCCA108030.2035693126845748No Hit
CTTCTCACTACTGCACTTGACTAG106860.2013645908865469No Hit
AACGGAATCCCAAAAGCAGCTG103520.1950707696853391No Hit
AACATTCAACGCTGTCGGTGAGTT101290.19086860762585006No Hit
CAACGGAATCCCAAAAGCAGCT99950.18834354163494632No Hit
TGTAAACATCCTACACTCAGCT99870.18819279142653414No Hit
TGTCAGTTTGTCAAATACCCCAA98060.18478206796120894No Hit
CCCCCACTGCTAAATTTGACTGGCTT97700.18410369202335422No Hit
TGTCAGTTTGTCAAATACCCCA96400.18165400113665656No Hit
GGAATACCGGGTGCTGTAGGCTTT94970.17895934116128914No Hit
TTCACAGTGGCTAAGTTCCG93030.1753036486072942No Hit
TTCAAGTAATCCAGGATAGGCTT91970.17330620834583305No Hit
ACCACAGGGTAGAACCACGGAC89530.16870832698926205No Hit
AGCTCGGTCTGAGGCCCCTCAGT88170.1661455734462553No Hit
GGAATACCGGGTGCTGTAGGCTT86270.1625652559964664No Hit
TAGCAGCACGTAAATATTGGC85630.1613592543291691No Hit
TAGCTTATCAGACTGATGTTGACA81540.15365214992409726No Hit
AGCAGCATTGTACAGGGCTATGT81280.15316221174675773No Hit
TGTAAACATCCCCGACTGGAAGC80240.15120245903739962No Hit
TTCAAGTAATCCAGGATAGGC79770.15031680156297816No Hit
TATTGCACTTGTCCCGGCCTGTT78870.14862086171834132No Hit
TTATAAAGCAATGAGACTGATT77030.14515360692486157No Hit
CCCCCCACTGCTAAATTTGACTGGCT76650.1444375434349038No Hit
AGCAGCATTGTACAGGGCTATG75840.14291119757473067No Hit
GGGCTACGCCTGTCTGAGCGTCGCT74010.1394627865573024No Hit
TAGTGGGTTATCAGAACT73550.13859597285893246No Hit
TGAGGTAGTAGGTTGTGTGGTT72880.13733343986348062No Hit
AATGACACGATCACTCCCGTTG69860.13164261949592143No Hit
TTCACAGTGGCTAAGTTCTGC69660.13126574397489105No Hit
CGCGACCTCAGATCAGACGTGG68940.12990899209918158No Hit
CCCCCCACTGCTAAATTTGACTGG68760.1295698041302542No Hit
TTCACAGTGGCTAAGTTCCGC68020.1281753647024417No Hit
CAAAGAATTCTCCTTTTGGGCT66970.12619676821703205No Hit
CAAAGTGCTGTTCGTGCAGGTAG65640.12369054600217984No Hit
ATCCCACCGCTGCCACCA63360.1193941650624332No Hit
CAAAGTGCTGTTCGTGCAGGT63010.11873463290062998No Hit
TAGCAGCACGTAAATATTGGCGT61300.11551234719582No Hit
TGAGGTAGTAGGTTGTATAGT60460.11392947000749229No Hit
CACTAGATTGTGAGCTCCTGGA59710.11251618680362825No Hit
AGAGGTAGTAGGTTGCATAGTT59310.11176243576156746No Hit
CGCGACCTCAGATCAGACGTGGCG57200.10778639901469665No Hit
CAAAGAATTCTCCTTTTGGGCTT56520.10650502224319326No Hit
GGGAATACCGGGTGCTGTAGGCTT53250.10034310747434609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)