FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8714037
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG102617511.776114790423772No Hit
GCCTGTCTGAGCGTCGCT4374135.019636707991944No Hit
AAGCTGCCAGTTGAAGAACTGT3227233.7034843896118415No Hit
TTCAAGTAATCCAGGATAGGCT3223223.6988826189285176No Hit
TAGCAGCACGTAAATATTGGCG3210473.6842510537882727No Hit
CCCCCCACTGCTAAATTTGACTG3209433.6830575771023235No Hit
AACATTCAACGCTGTCGGTGAGT2205532.5310083030402555No Hit
CCCCCACTGCTAAATTTGACTG1849482.1224146741630774No Hit
AACATTCAACGCTGTCGGTGAG1697891.948453971448595No Hit
TGAGGTAGTAGGTTGTATAGTT1273911.4619056586516674No Hit
CATAAAGTAGAAAGCACTACT1140381.3086701376181902No Hit
TAGCTTATCAGACTGATGTTGAC987081.13274708381431No Hit
TAGCTTATCAGACTGATGTTGACT866600.9944874000420242No Hit
TATTGCACTTGTCCCGGCCTGTAA844070.9686325637589098No Hit
TATTGCACTTGTCCCGGCCTGT798750.916624522021194No Hit
TAGCTTATCAGACTGATGTTGA724930.8319106287935202No Hit
TATTGCACTTGTCCCGGCCTGTAT690970.792939024702328No Hit
TGTAAACATCCTTGACTGGAAGCT682470.783184647942165No Hit
TGAGGTAGTAGATTGTATAGTT615970.7068709944655961No Hit
CAACGGAATCCCAAAAGCAGCTGT574420.6591893057144467No Hit
TCGTACGACTCTTAGCGG547440.628227766303953No Hit
AGCAGCATTGTACAGGGCTATGA479340.5500779948490006No Hit
TTCAAGTAATTCAGGATAGGTT468710.5378792860301144No Hit
TATTGCACTTGTCCCGGCCTGTA418660.480443220518802No Hit
CCCATAAAGTAGAAAGCACTAC414570.4757496439365589No Hit
TGTAAACATCCCCGACTGGAAGCT403730.46330994463300995No Hit
CATTGCACTTGTCTCGGTCTGA396030.4544736268620388No Hit
CCCATAAAGTAGAAAGCACT375690.43113197706183715No Hit
TTAATGCTAATCGTGATAGGGGTT351640.40353282869925844No Hit
ACTGGACTTGGAGTCAGAAGGC314940.3614168725700843No Hit
CAACGGAATCCCAAAAGCAGCT302360.3469803949650432No Hit
TCCTGTCTGAGCGTCGCT285100.32717327227322995No Hit
CAACACCAGTCGATGGGCTGT283450.3252797756080219No Hit
CACCCGGCTGTGTGCACATGTGC273800.3142056890508957No Hit
ACCGGGTGCTGTAGGCTT266400.3057136434008715No Hit
TGAGGTAGTAGTTTGTACAGTT247180.2836572761855383No Hit
TAGCAGCACGTAAATATTGGC234320.26889947793428004No Hit
AACGGAATCCCAAAAGCAGCTG230800.26486001838183615No Hit
CCCCCACTGCTAAATTTGACTGGCTT229200.26302390040345247No Hit
TGATGTAGTAGGTTGTATAGTT227790.2614058214350019No Hit
TTCTCACTACTGCACTTGACTAG227610.2611992581624338No Hit
TGTAAACATCCTACACTCTCAGCT219080.25141045419017616No Hit
AACCGGGCGGAAACACCA206150.2365723257773636No Hit
TTCACAGTGGCTAAGTTCCGC199700.22917047517700462No Hit
TGAGAACTGAATTCCATGGGTT192960.22143582819306368No Hit
CGCCTGTCTGAGCGTCGCT190880.21904887482116497No Hit
TGAGGTAGTAGTTTGTGCTGTT172790.19828926592806526No Hit
CAAAGAATTCTCCTTTTGGGCT164680.18898244292513333No Hit
ACCGGGTGCTGTAGGCTTT163630.18777749050181908No Hit
TTCAAGTAATCCAGGATAGGC143930.16517028789297084No Hit
TTCACAGTGGCTAAGTTCTGC141590.16248496534958481No Hit
AACATTCAACGCTGTCGGTGAGTT141050.16186527553188035No Hit
TGAGAACTGAATTCCATAGGCTGT138520.15896191397856127No Hit
CTCCCACTGCTTCACTTGACTAG137720.15804385498936946No Hit
CTTCTCACTACTGCACTTGACTAG135010.1549339301634822No Hit
TTCAAGTAATCCAGGATAGGCTT129590.14871408051170773No Hit
TATTGCACTTGTCCCGGCCTGTT127230.14600580649359188No Hit
ACCGGGTGCTGTAGGCTTA126260.14489265996919684No Hit
TAGCTTATCAGACTGATGTTGACA124920.14335491116230054No Hit
TGTAAACATCCCCGACTGGAAGC123560.14179421088067448No Hit
ATCACATTGCCAGGGATTTCC120160.1378924601766093No Hit
CAAAGAATTCTCCTTTTGGGCTT119120.13669898349065995No Hit
TGTCAGTTTGTCAAATACCCCA119060.13663012906647057No Hit
TGAGGTAGTAGGTTGTGTGGTT116500.13369234030105678No Hit
CACTAGATTGTGAGCTCCTGGA115990.13310707769544702No Hit
TGTAAACATCCTACACTCAGCT115190.1321890187062552No Hit
CCCCCCACTGCTAAATTTGACTGGCT113620.13038732793996627No Hit
CCCATAAAGTAGAAAGCACTA112100.12864301586050186No Hit
AGCTCGGTCTGAGGCCCCTCAGT111180.12758724802293128No Hit
CTACGCCTGTCTGAGCGTCGCT110880.12724297590198436No Hit
TTCACAGTGGCTAAGTTCCG110190.1264511500238064No Hit
TGATGTAGTAGATTGTATAGTT109050.1251429159642081No Hit
CGCGACCTCAGATCAGACGTGG108750.12479864384326116No Hit
ATCCCGGACGAGCCCCCA107780.1236854973188661No Hit
TGAGGTAGTAGGTTGTATAGT107310.12314613766271593No Hit
TCGTTTCCCGGCCAATGCACCA105820.12143625279534619No Hit
TTATAAAGCAATGAGACTGATT104450.11986407677635522No Hit
ACCACAGGGTAGAACCACGGAC102470.11759188077810549No Hit
AGAGGTAGTAGGTTGCATAGTT100420.11523935461830148No Hit
TGTCAGTTTGTCAAATACCCCAA100370.115181975931477No Hit
CCCCCCACTGCTAAATTTGACTGG95240.10929492266328455No Hit
TAGCAGCACGTAAATATTGGCGT95130.10916868955227067No Hit
CACTAGATTGTGAGCTCCTGGAA91890.10545055064604386No Hit
TATTGCACTTGTCCCGGCCTGTAG91840.10539317195921936No Hit
AGCAGCATTGTACAGGGCTATGT91430.10492266672725856No Hit
TGTAAACATCCCCGACTGGA91150.10460134608104144No Hit
ACCCCCCACTGCTAAATTTGACTG90860.10426854969745941No Hit
CAAAGTGCTGTTCGTGCAGGT90630.10400460773806676No Hit
TTCACAGTGGCTAAGTTCTG87680.10061926521542197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)