FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7981988
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGTCTGAGCGTCGCT6169407.72915218614711No Hit
CAACGGAATCCCAAAAGCAGCTG5932117.431870356106775No Hit
TTCAAGTAATCCAGGATAGGCT2965583.7153400882086016No Hit
CCCCCCACTGCTAAATTTGACTG2669833.3448183585342397No Hit
AAGCTGCCAGTTGAAGAACTGT2653413.324247042215548No Hit
TAGCAGCACGTAAATATTGGCG2586223.2400700176447272No Hit
CCCCCACTGCTAAATTTGACTG1590881.9930874363629711No Hit
AACATTCAACGCTGTCGGTGAGT1585911.9868609173554257No Hit
CATAAAGTAGAAAGCACTACT1332411.66927086334883No Hit
AACATTCAACGCTGTCGGTGAG1273271.5951790456212163No Hit
TAGCTTATCAGACTGATGTTGAC954621.1959677213245623No Hit
TGAGGTAGTAGGTTGTATAGTT931111.166513906059493No Hit
TATTGCACTTGTCCCGGCCTGT803361.0064660583303309No Hit
TAGCTTATCAGACTGATGTTGA770320.9650728615477747No Hit
TTCAAGTAATTCAGGATAGGTT722380.905012635949841No Hit
TAGCTTATCAGACTGATGTTGACT649860.8141580769101632No Hit
ACCGGGTGCTGTAGGCTT625770.7839776256240926No Hit
TGAGGTAGTAGATTGTATAGTT563820.7063653816568003No Hit
TGTAAACATCCTTGACTGGAAGCT546490.6846539984775722No Hit
TATTGCACTTGTCCCGGCCTGTAA545650.6836016290678463No Hit
TCCTGTCTGAGCGTCGCT542740.6799559207555812No Hit
AACCGGGCGGAAACACCA493330.618054048690627No Hit
TATTGCACTTGTCCCGGCCTGTAT484790.607354959691746No Hit
TCGTACGACTCTTAGCGG477450.598159255563902No Hit
CAACGGAATCCCAAAAGCAGCT440180.5514666271109403No Hit
CATTGCACTTGTCTCGGTCTGA411990.5161496108488262No Hit
ACCGGGTGCTGTAGGCTTT395270.4952024483123753No Hit
TATTGCACTTGTCCCGGCCTGTA387280.4851924107127197No Hit
TTAATGCTAATCGTGATAGGGGTT382280.4789283070833983No Hit
AGCAGCATTGTACAGGGCTATGA354710.44438803967131996No Hit
CGCCTGTCTGAGCGTCGCT349000.43723443332663486No Hit
CCCATAAAGTAGAAAGCACT346470.4340647968901983No Hit
ACCGGGTGCTGTAGGCTTA316610.39665557001589075No Hit
CAACGGAATCCCAAAAGCAGCTGT315800.3956407852279407No Hit
CAACACCAGTCGATGGGCTGT294800.36933154998479073No Hit
CCCATAAAGTAGAAAGCACTAC269020.3370338316720095No Hit
TTCTCACTACTGCACTTGACTAG267540.3351796569977304No Hit
TGTAAACATCCCCGACTGGAAGCT248210.3109626323667738No Hit
TGAGGTAGTAGTTTGTACAGTT242770.3041472876180721No Hit
ACTGGACTTGGAGTCAGAAGGC240090.30078972807275584No Hit
TGAGAACTGAATTCCATGGGTT222740.2790532884790105No Hit
TTCACAGTGGCTAAGTTCCG218150.27330284134729343No Hit
CACCCGGCTGTGTGCACATGTGC211580.2650718091783651No Hit
TGTAAACATCCTACACTCTCAGCT190930.23920106118926762No Hit
AACGGAATCCCAAAAGCAGCTG186150.23321257811963636No Hit
TAGCAGCACGTAAATATTGGC177390.22223786856106525No Hit
TGAGGTAGTAGTTTGTGCTGTT175740.2201707143633892No Hit
TTCAAGTAATCCAGGATAGGC157030.19673043858246841No Hit
ATCCCGGACGAGCCCCCA154750.19387400732749785No Hit
CTCCCACTGCTTCACTTGACTAG152120.1905790888184748No Hit
TTCACAGTGGCTAAGTTCTGC146330.1833252568157206No Hit
CTACGCCTGTCTGAGCGTCGCT136170.17059659824093948No Hit
TGTCAGTTTGTCAAATACCCCA134700.16875495177391897No Hit
TCGTTTCCCGGCCAATGCACCA133090.16673791040527747No Hit
TTCACAGTGGCTAAGTTCTG132780.16634953598025956No Hit
TTATAAAGCAATGAGACTGATT126640.15865721672345287No Hit
TTCACAGTGGCTAAGTTCCGC122350.15328261580949507No Hit
TGTCAGTTTGTCAAATACCCCAA122220.15311974911513274No Hit
TGTAAACATCCTACACTCAGCT118690.14869729195283182No Hit
TATTGCACTTGTCCCGGCCTGTT117200.146830589071294No Hit
AATGACACGATCACTCCCGTTG116770.14629187615917238No Hit
CTTCTCACTACTGCACTTGACTAG112120.14046625978390348No Hit
TTCAAGTAATCCAGGATAGGCTT111500.1396895109338676No Hit
TGAGGTAGTAGGTTGTGTGGTT105240.13184685318995717No Hit
CCCCCACTGCTAAATTTGACTGGCTT104540.1309698786818522No Hit
AACATTCAACGCTGTCGGTGAGTT104470.1308821812310417No Hit
AGCTCGGTCTGAGGCCCCTCAGT103970.13025577086810955No Hit
CAAAGTGCTGTTCGTGCAGGT103940.1302181862463336No Hit
CGCGACCTCAGATCAGACGTGG101550.127223944711518No Hit
CCCATAAAGTAGAAAGCACTA100000.12528207258642834No Hit
CAAAGAATTCTCCTTTTGGGCT99730.12494381099044499No Hit
GGAATACCGGGTGCTGTAGGCTTT97170.12173658993223242No Hit
TACGCCTGTCTGAGCGTCGCT96770.1212354616418867No Hit
GGAATACCGGGTGCTGTAGGCTT96290.12063410769347184No Hit
ATCCCACCGCTGCCACCA96060.12034595892652306No Hit
TGAGGTAGTAGGTTGTATAGT95980.12024573326845393No Hit
CCCTGTCTGAGCGTCGCT92800.11626176336020551No Hit
CACTAGATTGTGAGCTCCTGGA91120.1141570245407535No Hit
CAACGGAATCCCAAAAGCAGCTGA90660.11358072700685592No Hit
AGAGGTAGTAGGTTGCATAGTT89510.112139983172112No Hit
AATGCACCCGGGCAAGGATTCT89390.1119896446850083No Hit
TAGCTTATCAGACTGATGTTGACA88840.11130059328578294No Hit
ACCACAGGGTAGAACCACGGAC87310.10938377757521059No Hit
TGTCAGTTTGTCAAATACC86560.10844416203081236No Hit
TGAGAACTGAATTCCATAGGCTGT85980.10771752600981109No Hit
AGCAGCATTGTACAGGGCTATG83390.1044727203298226No Hit
ACGCCTGTCTGAGCGTCGCT83260.10430985363546023No Hit
TCAGTGCACTACAGAACTTTGT81200.10172904294017981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)