FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7068526
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6368379.009473828065428No Hit
CCCCCCACTGCTAAATTTGACTG3614715.1138101493861665No Hit
GCCTGTCTGAGCGTCGCT2729953.862120617509223No Hit
TTCAAGTAATCCAGGATAGGCT2405723.4034252685779185No Hit
TAGCAGCACGTAAATATTGGCG2293933.2452734841747772No Hit
AACATTCAACGCTGTCGGTGAGT2188033.0954544129851116No Hit
AAGCTGCCAGTTGAAGAACTGT1896702.6833034213922393No Hit
CCCCCACTGCTAAATTTGACTG1788162.529749483838639No Hit
AACATTCAACGCTGTCGGTGAG1558512.204858551839521No Hit
TATTGCACTTGTCCCGGCCTGTAA916981.2972718781822405No Hit
TAGCTTATCAGACTGATGTTGAC854851.2093751936400885No Hit
TCCTGTCTGAGCGTCGCT798281.1293443640159206No Hit
TGAGGTAGTAGGTTGTATAGTT792631.1213511841082568No Hit
TATTGCACTTGTCCCGGCCTGTAT772271.0925474419985157No Hit
TAGCTTATCAGACTGATGTTGACT690840.9773466207806267No Hit
TATTGCACTTGTCCCGGCCTGT651020.9210123864579405No Hit
CATAAAGTAGAAAGCACTACT629600.8907090389141951No Hit
TGTAAACATCCTTGACTGGAAGCT585560.8284046772976431No Hit
TAGCTTATCAGACTGATGTTGA581450.8225901694356078No Hit
TGAGGTAGTAGATTGTATAGTT498820.7056916816886576No Hit
TATTGCACTTGTCCCGGCCTGTA436270.617200813861334No Hit
CAACGGAATCCCAAAAGCAGCT427660.6050200565153188No Hit
TGTAAACATCCCCGACTGGAAGCT424560.6006344179819103No Hit
TTCAAGTAATTCAGGATAGGTT409820.5797814141166066No Hit
AGCAGCATTGTACAGGGCTATGA397920.562946221036748No Hit
CAACGGAATCCCAAAAGCAGCTGT352930.4992978734180224No Hit
TTCTCACTACTGCACTTGACTAG295020.4173713161697361No Hit
ACTGGACTTGGAGTCAGAAGGC288390.40799170859667205No Hit
CATTGCACTTGTCTCGGTCTGA277070.39197705433919316No Hit
CACCCGGCTGTGTGCACATGTGC265760.37597654730278984No Hit
CCCATAAAGTAGAAAGCACTAC254790.3604570457829539No Hit
CCCATAAAGTAGAAAGCACT252080.3566231488714903No Hit
TGTCAGTTTGTCAAATACCCCAA235840.3336480618448599No Hit
CCCCCACTGCTAAATTTGACTGGCTT215680.305127264156629No Hit
CGCCTGTCTGAGCGTCGCT212190.30018988400127555No Hit
TGAGGTAGTAGTTTGTACAGTT205570.290824423649287No Hit
ACCGGGTGCTGTAGGCTT205520.29075368754390946No Hit
TCGTACGACTCTTAGCGG203010.2872027350539561No Hit
AACCGGGCGGAAACACCA201280.2847552658078926No Hit
CTCCCACTGCTTCACTTGACTAG177730.2514385601750634No Hit
CTTCTCACTACTGCACTTGACTAG177590.2512404990800062No Hit
CAACACCAGTCGATGGGCTGT173410.24532696067044246No Hit
AACGGAATCCCAAAAGCAGCTG166050.23491460595886612No Hit
TGTAAACATCCTACACTCTCAGCT160950.2276995232103553No Hit
TAGCAGCACGTAAATATTGGC158160.22375244853028764No Hit
AACATTCAACGCTGTCGGTGAGTT150660.21314203272365415No Hit
TCGTTTCCCGGCCAATGCACCA145520.20587036109084128No Hit
TGTCAGTTTGTCAAATACCCCA145230.20546009167965149No Hit
ACCGGGTGCTGTAGGCTTT142580.20171107809464095No Hit
TGTAAACATCCCCGACTGGAAGC140640.19896651720599173No Hit
CAAAGAATTCTCCTTTTGGGCT138740.1962785452016446No Hit
ACCGGGTGCTGTAGGCTTA136850.19360472041837293No Hit
TGAGGTAGTAGTTTGTGCTGTT135040.19104407340370538No Hit
CCCCCCACTGCTAAATTTGACTGGCT123210.17430791087137543No Hit
TTCACAGTGGCTAAGTTCTGC122560.1733883415014672No Hit
TGATGTAGTAGGTTGTATAGTT120070.16986568345366487No Hit
CTACGCCTGTCTGAGCGTCGCT116040.1641643533602338No Hit
TAGCTTATCAGACTGATGTTGACA113930.1611792897133009No Hit
TTCACAGTGGCTAAGTTCCG112620.1593260037524089No Hit
CAACGGAATCCCAAAAGCAGCTGA112450.15908550099412522No Hit
TATTGCACTTGTCCCGGCCTGTT112200.15873182046723744No Hit
CCCCCCACTGCTAAATTTGACTGG107560.15216750988820016No Hit
GGAATACCGGGTGCTGTAGGCTTT107520.15211092100389814No Hit
TTCAAGTAATCCAGGATAGGC107030.15141770717119807No Hit
CACTAGATTGTGAGCTCCTGGA103390.14626811869971193No Hit
TTCACAGTGGCTAAGTTCCGC102390.1448533965921608No Hit
TTCAAGTAATCCAGGATAGGCTT102230.1446270410549526No Hit
CAAAGAATTCTCCTTTTGGGCTT102020.14432994941236688No Hit
TATTGCACTTGTCCCGGCCTGTAG99600.14090632191209312No Hit
AGCTCGGTCTGAGGCCCCTCAGT97840.13841641100280314No Hit
GGAATACCGGGTGCTGTAGGCTT96170.13605382508319275No Hit
CACTAGATTGTGAGCTCCTGGAA89530.12666007028905318No Hit
CGCGACCTCAGATCAGACGTGGCG87840.12426918992729179No Hit
TTCACAGTGGCTAAGTTCTG87470.12374574274749786No Hit
TTATAAAGCAATGAGACTGATT84590.11967134307775057No Hit
TGAGGTAGTAGGTTGTGTGGTT84510.11955816530914648No Hit
ACTGGACTTGGAGTCAGAAGGCT84050.11890739313967297No Hit
ACCCCCCACTGCTAAATTTGACTG83830.11859615427601171No Hit
AATGCACCCGGGCAAGGATTCT83820.11858200705493621No Hit
CCCATAAAGTAGAAAGCACTA81130.11477640458562365No Hit
TAGCAGCACGTAAATATTGGCGT77910.11022099939930899No Hit
ACTGGACTTGGAGTCAGAAGGCA76920.10882042451283336No Hit
ACCACAGGGTAGAACCACGGAC75820.10726423019452713No Hit
ATCACATTGCCAGGGATTTCC75700.10709446354162097No Hit
TGTAAACATCCTACACTCAGCT74910.10597683307665559No Hit
AGCAGCATTGTACAGGGCTATGT73370.10379816103102683No Hit
ACTGGACTTGGAGTCAGAAGG72930.10317568330370434No Hit
TGATGTAGTAGATTGTATAGTT72910.10314738886155331No Hit
TGAGGTAGTAGGTTGTATAGT72390.10241173336562673No Hit
ATCCCGGACGAGCCCCCA72100.10200146395443689No Hit
ATCCCACCGCTGCCACCA70790.10014817799354492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)