FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8844469
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG131378714.854334386835433No Hit
CAACGGAATCCCAAAAGCAGCTG5189805.867848030220921No Hit
CCCCCACTGCTAAATTTGACTG4500755.088773560063357No Hit
TAGCAGCACGTAAATATTGGCG3162763.5759749963508267No Hit
AACATTCAACGCTGTCGGTGAGT3138873.548963764811658No Hit
TTCAAGTAATCCAGGATAGGCT2714353.068980172806304No Hit
AAGCTGCCAGTTGAAGAACTGT2620452.9628121258608062No Hit
AACATTCAACGCTGTCGGTGAG1887712.1343395516452146No Hit
TATTGCACTTGTCCCGGCCTGTAA1060571.1991336054205177No Hit
TAGCTTATCAGACTGATGTTGAC1050061.187250472583487No Hit
TGAGGTAGTAGGTTGTATAGTT994131.1240132109683463No Hit
TAGCTTATCAGACTGATGTTGACT989521.1188009138818849No Hit
TATTGCACTTGTCCCGGCCTGTAT978811.1066916510194111No Hit
TAGCTTATCAGACTGATGTTGA959781.085175379098508No Hit
TATTGCACTTGTCCCGGCCTGT829470.9378403610211081No Hit
TTCTCACTACTGCACTTGACTAG781330.8834108638969733No Hit
TTCAAGTAATTCAGGATAGGTT727780.8228645495846048No Hit
TGAGGTAGTAGATTGTATAGTT695320.7861636464552026No Hit
TGTAAACATCCTTGACTGGAAGCT680210.7690795230329825No Hit
CATAAAGTAGAAAGCACTACT603510.6823586582755844No Hit
CCCCCCACTGCTAAATTTGACTGG538630.6090020780218688No Hit
TATTGCACTTGTCCCGGCCTGTA523270.5916352920678449No Hit
AGCAGCATTGTACAGGGCTATGA470990.5325249034170395No Hit
CTTCTCACTACTGCACTTGACTAG458220.5180865012925027No Hit
TGTAAACATCCCCGACTGGAAGCT439460.49687550490594745No Hit
GACCCGCGGGCGCTCTCCAGTCCT313010.3539047963195982No Hit
ACTGGACTTGGAGTCAGAAGGC300490.3397490567268651No Hit
TGAGGTAGTAGTTTGTACAGTT291370.3294375275666634No Hit
CGCCTGTCTGAGCGTCGCT284600.3217830262054172No Hit
CATTGCACTTGTCTCGGTCTGA284050.3211611686354489No Hit
TGATGTAGTAGGTTGTATAGTT274830.31073657446252567No Hit
CAACGGAATCCCAAAAGCAGCTGT271350.3068019120198171No Hit
CTCCCACTGCTTCACTTGACTAG230510.26062616082435247No Hit
CCCATAAAGTAGAAAGCACT210390.23787748026478467No Hit
CCCATAAAGTAGAAAGCACTAC204020.2306752389544245No Hit
AACATTCAACGCTGTCGGTGAGTT199100.2251124403285262No Hit
CCCCCACTGCTAAATTTGACTGG198370.2242870657356592No Hit
TGATGTAGTAGATTGTATAGTT193820.2191426076568305No Hit
TAGCAGCACGTAAATATTGGC182520.2063662612192999No Hit
CAACACCAGTCGATGGGCTGT178620.201956725723161No Hit
TGTAAACATCCTACACTCTCAGCT172080.19456227389117425No Hit
TGAGGTAGTAGTTTGTGCTGTT171720.1941552398453768No Hit
ACCCCCCACTGCTAAATTTGACTG167510.18939520280980124No Hit
AGCTCGGTCTGAGGCCCCTCAGT165080.18664772300066856No Hit
TGTAAACATCCCCGACTGGAAGC157670.17826960555800467No Hit
CAAAGAATTCTCCTTTTGGGCT155130.17539775423487833No Hit
TTCAAGTAATCCAGGATAGGCTT145930.1649957730644994No Hit
TATTGCACTTGTCCCGGCCTGTT140890.15929729642333532No Hit
CAAAGAATTCTCCTTTTGGGCTT140360.1586980518559113No Hit
CCCCCACTGCTAAATTTGACTGGCTT138060.1560975565633166No Hit
TAGCTTATCAGACTGATGTTGACA136670.1545259528864876No Hit
ACCGGGTGCTGTAGGCTT130720.14779858462955775No Hit
TATTGCACTTGTCCCGGCCTGTAG129450.14636265896799458No Hit
CCCCCCACTGCTAAATTTGACTGGCT125120.1414669439171532No Hit
TGTCAGTTTGTCAAATACCCCA120960.1367634393879384No Hit
AACGGAATCCCAAAAGCAGCTG117660.13303229396812855No Hit
TGAGAACTGAATTCCATAGGCTGT116370.13157375530402107No Hit
AACATTCAACGCTGTCGGTGA111120.1256378421361418No Hit
CAACGGAATCCCAAAAGCAGCT109440.12373834992242044No Hit
TTCAAGTAATCCAGGATAGGC108920.1231504118562686No Hit
CCCCCCACTGCTAAATTTGACTGT108200.12233634376467371No Hit
TTCACAGTGGCTAAGTTCCG107790.12187277721251552No Hit
CGCGACCTCAGATCAGACGTGGCG107570.1216240341845282No Hit
CACTAGATTGTGAGCTCCTGGA107380.12140921066035734No Hit
TTATAAAGCAATGAGACTGATT105300.11905745839574994No Hit
TGAGGTAGTAGGTTGTGTGGTT104190.11780243675454118No Hit
CCCCCACAACCGCGCTTGACTAG103310.1168074646425919No Hit
CTACGCCTGTCTGAGCGTCGCT102310.11567681451537677No Hit
CAAAGTGCTGTTCGTGCAGGT101620.11489666592759835No Hit
CACCCGGCTGTGTGCACATGTGC101480.11473837490978825No Hit
ACCGGGTGCTGTAGGCTTT99280.11225094462991503No Hit
TGTCAGTTTGTCAAATACCCCAA99210.11217179912100998No Hit
TTCACAGTGGCTAAGTTCTGC94510.10685774352309901No Hit
GGAATACCGGGTGCTGTAGGCTTT94210.10651854848493449No Hit
AATGACACGATCACTCCCGTTG93690.10593061041878263No Hit
TGAGGTAGTAGGTTGTATAGT91770.10375976217452965No Hit
ACCGGGTGCTGTAGGCTTA91760.1037484556732575No Hit
AGCAGCATTGTACAGGGCTATG90810.10267433805240314No Hit
GGAATACCGGGTGCTGTAGGCTT90450.1022673040066057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)